BUSCADOR RECOLECTA

Experimental and computational methods; Mechanical properties of layered hybrid organic–inorganic metal-halide perovskites; Additional Raman spectroscopy characterization; Characterization of the SiO2 rings and flake/ring structures by SEM and AFM; z-depth penetration of the lasers; Supplementary photoluminescence and Raman spectroscopy data; Evolution of the temperature-dependent photoluminescence and Raman maps with the SiO2 ring dimensions; Power-dependence of the photoluminescence maps; and Additional finite element simulations., Peer reviewed

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Mononucleosomal DNA was isolated after MNase treatment and was then prepared for sequencing..-- Organism: Saccharomyces cerevisiae, Resources available on the publisher's site: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE192701, The stability of the genome is occasionally challenged by the formation of DNA-RNA hybrids and R-loops, which can be influenced by the chromatin context. This is mainly due to the fact that DNA-RNA hybrids hamper the progression of replication forks, leading to fork stalling and, ultimately, DNA breaks. Through a specific screening of chromatin modifiers performed in the yeast Saccharomyces cerevisiae, we have found that the Rtt109 histone acetyltransferase is involved in several steps of R-loop-metabolism and their associated genetic instability. On one hand, Rtt109 prevents DNA-RNA hybridization by the acetylation of histone H3 lysines 14 and 23, and on the other hand, it is involved in the repair of replication-born DNA breaks, such as those that can be caused by R-loops, by acetylating lysines 14 and 56. In addition, Rtt109 loss renders cells highly sensitive to replication stress in combination with R-loop-accumulating THO-complex mutants. Our data evidence that the chromatin context simultaneously influences the occurrence of DNA-RNA hybrid-associated DNA damage and its repair, adding complexity to the source of R-loop-associated genetic instability., Peer reviewed

Peer reviewed

The following is the Supplementary data to this article: Local Young's Modulus (E) of conductive PEDOT:PSS/PEO blends; 1D GIWAXS intensity profiles obtained at different incidence angles; histograms of nanomechanical properties calculated from Fig. 3; additional AFM height images for different regions complementing Fig. 5; Polarized Optical Microscope images; I–V curves for PEDOT:PSS/PEO blends., Peer reviewed

1 table., Peer reviewed

Computational details, crystal structure of the TMH monolayers, phonon dispersion curves, thermal stability, spin polarization band structures, magnetic configurations, detailed description of electrides, charge density difference, and structural information of the predicted TMH monolayers., Peer reviewed

Multimedia component 1., Peer reviewed

Analysis of the pH influence in the JC1 J-aggregates formation using absorbance and photoluminescence spectra and estimation of the mean lifetime of monomer and J-aggregates, results of the pH influence in the exciton coherence length applying the four explained methods to different experiments, and summary of reported J-aggregates’ exciton coherence length., Peer reviewed

1 table., Peer reviewed