Dataset.

Additional file 2 of Overexpression of wild type RRAS2, without oncogenic mutations, drives chronic lymphocytic leukemia [Dataset]

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/329846
Digital.CSIC. Repositorio Institucional del CSIC
  • Hortal, Alejandro
  • Oeste, Clara L.
  • Cifuentes, Claudia
  • Alcoceba, Miguel
  • Fernández-Pisonero, Isabel
  • Clavaín, Laura
  • Tercero, Rut
  • Mendoza, Pilar
  • Domínguez, Verónica
  • García-Flores, Marta
  • Pintado, Belén
  • Abia, David
  • García-Macías, Carmen
  • Navarro-Bailón, Almudena
  • Bustelo, Xosé R.
  • González, Marcos
  • Alarcón, Balbino
Additional file 2: Figure S2. a, Flow cytometry analysis of GFP populations in 23 wk-old Rosa26-RRAS2fl/flxSox2-Cre mouse spleen. Representative two-color contour plots of GFPhigh and GFPlow populations in total B cells (CD19+), CD5+ leukemic and CD23+ follicular B cells. Bottom, representation of GFP populations in T lymphocytes (CD3+). b, Percentage of GFPhigh cells in the indicated populations determined by flow cytometry. Data show means ± SEM from n = 8 mice (23 wk-old mice). ****p < 0.0001 (one-way ANOVA test). c, Western blot analysis of R-RAS2 expression of sorted GFPlow and GFPhigh leukemic cells from the spleen of a 25 wk-old Rosa26-RRAS2fl/flxSox2-Cre mouse (β-actin as loading control). d, Dot plot representation of GFPlow CD5+ leukemic B cell evolution in mb1-Cre mice over time, showing each mouse individually (n = 14). Data points were adjusted to a linear fit. These data were retrieved from the same mice as in Fig. 2i. e, Percentage of CD5+ cells in the indicated populations comparing GFPhigh and GFPlow distribution. Data show means ± SEM from n = 4 30 wk-old mice. Two-way ANOVA test. f, Heatmap of RNAseq expression data showing the genes differentially regulated in wild-type, follicular B cells (n = 6, 12wk-old), leukemic CD19 + CD5+ B cells (n = 6, 54wk-old), CD19+ GFPhigh (n = 2, 54wk-old) and CD19+ GFPlow (n = 2, 54wk-old) populations. Only genes significantly different between GFPhigh GFPlow populations (p < 0.05) and with a difference of 2-fold or more were used. Gene expression is shown in normalized log2 fold change., Peer reviewed
 
DOI: http://hdl.handle.net/10261/329846
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/329846

HANDLE: http://hdl.handle.net/10261/329846
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/329846
 
Ver en: http://hdl.handle.net/10261/329846
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/329846

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/329846
Dataset. 2022

ADDITIONAL FILE 2 OF OVEREXPRESSION OF WILD TYPE RRAS2, WITHOUT ONCOGENIC MUTATIONS, DRIVES CHRONIC LYMPHOCYTIC LEUKEMIA [DATASET]

Digital.CSIC. Repositorio Institucional del CSIC
  • Hortal, Alejandro
  • Oeste, Clara L.
  • Cifuentes, Claudia
  • Alcoceba, Miguel
  • Fernández-Pisonero, Isabel
  • Clavaín, Laura
  • Tercero, Rut
  • Mendoza, Pilar
  • Domínguez, Verónica
  • García-Flores, Marta
  • Pintado, Belén
  • Abia, David
  • García-Macías, Carmen
  • Navarro-Bailón, Almudena
  • Bustelo, Xosé R.
  • González, Marcos
  • Alarcón, Balbino
Additional file 2: Figure S2. a, Flow cytometry analysis of GFP populations in 23 wk-old Rosa26-RRAS2fl/flxSox2-Cre mouse spleen. Representative two-color contour plots of GFPhigh and GFPlow populations in total B cells (CD19+), CD5+ leukemic and CD23+ follicular B cells. Bottom, representation of GFP populations in T lymphocytes (CD3+). b, Percentage of GFPhigh cells in the indicated populations determined by flow cytometry. Data show means ± SEM from n = 8 mice (23 wk-old mice). ****p < 0.0001 (one-way ANOVA test). c, Western blot analysis of R-RAS2 expression of sorted GFPlow and GFPhigh leukemic cells from the spleen of a 25 wk-old Rosa26-RRAS2fl/flxSox2-Cre mouse (β-actin as loading control). d, Dot plot representation of GFPlow CD5+ leukemic B cell evolution in mb1-Cre mice over time, showing each mouse individually (n = 14). Data points were adjusted to a linear fit. These data were retrieved from the same mice as in Fig. 2i. e, Percentage of CD5+ cells in the indicated populations comparing GFPhigh and GFPlow distribution. Data show means ± SEM from n = 4 30 wk-old mice. Two-way ANOVA test. f, Heatmap of RNAseq expression data showing the genes differentially regulated in wild-type, follicular B cells (n = 6, 12wk-old), leukemic CD19 + CD5+ B cells (n = 6, 54wk-old), CD19+ GFPhigh (n = 2, 54wk-old) and CD19+ GFPlow (n = 2, 54wk-old) populations. Only genes significantly different between GFPhigh GFPlow populations (p < 0.05) and with a difference of 2-fold or more were used. Gene expression is shown in normalized log2 fold change., Peer reviewed




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