Dataset.

Supplementary Materials - Exploring the anti-Inflammatory effect of Inulin by integrating transcriptomic and proteomic analyses in a murine macrophage cell model

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/334793
Digital.CSIC. Repositorio Institucional del CSIC
  • Farabegoli, Federica
  • Santaclara, Francisco J.
  • Costas Imbernón, Daniel
  • Alonso, Mercedes
  • Abril, Ana G.
  • Espiñeira, Montserrat
  • Ortea, Ignacio
  • Costas, Celina
Supplementary Table S1. Amplification system for the genes of interest and for the genes used for normalization in the qPCR validation. Supplementary Table S2. Normalized counts of all mapped genes from the RNA-Seq assay. Supplementary Table S3. Differentially expressed genes according to the differential expression analysis with DESeq2. Supplementary Table S4. diaPASEF-based protein quantification data for all the samples. A total of 6839 proteins were quantified by diaPASEF LC-MS/MS after processing of the runs as described in the Section 2 and Section 2.5.4. Supplementary Table S5. Differential abundance test for comparison of the LPS + I1 vs. LPS groups. Proteins with CV ≤ 20.0% and quantified in at least 50% of the samples of each group were considered. Fold changes, resulting p-values, and Benjamini–Hochberg corrected p-values for each of the 6065 considered proteins are shown. Supplementary Figure S1. Effect of inulin on the lysosome pathway (KEGG: 04142) from RAW 264.7 LPS-induced inflammation model cells. Pathway diagrams overlayed with the measured protein fold change showing coherent cascades. Differentially expressed genes are represented with positive values in red. Supplementary Figure S2. Effect of inulin on the NF-κB signaling pathway showing integrated transcriptomics data. Genes overexpressed (in red) or underexpressed (green) according to the transcriptomic analysis are highlighted. Supplementary Figure S3. Effect of Inulin on the IL-17 signaling pathway (KEGG: 04657) from RAW 264.7 LPS-induced inflammation model cells. Pathway diagram, overlayed with the measured protein perturbation showing coherent cascades. Differentially expressed genes are represented with negative fold-change values in blue and positive values in red., Peer reviewed
 
DOI: http://hdl.handle.net/10261/334793
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/334793

HANDLE: http://hdl.handle.net/10261/334793
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/334793
 
Ver en: http://hdl.handle.net/10261/334793
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/334793

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/334793
Dataset. 2023

SUPPLEMENTARY MATERIALS - EXPLORING THE ANTI-INFLAMMATORY EFFECT OF INULIN BY INTEGRATING TRANSCRIPTOMIC AND PROTEOMIC ANALYSES IN A MURINE MACROPHAGE CELL MODEL

Digital.CSIC. Repositorio Institucional del CSIC
  • Farabegoli, Federica
  • Santaclara, Francisco J.
  • Costas Imbernón, Daniel
  • Alonso, Mercedes
  • Abril, Ana G.
  • Espiñeira, Montserrat
  • Ortea, Ignacio
  • Costas, Celina
Supplementary Table S1. Amplification system for the genes of interest and for the genes used for normalization in the qPCR validation. Supplementary Table S2. Normalized counts of all mapped genes from the RNA-Seq assay. Supplementary Table S3. Differentially expressed genes according to the differential expression analysis with DESeq2. Supplementary Table S4. diaPASEF-based protein quantification data for all the samples. A total of 6839 proteins were quantified by diaPASEF LC-MS/MS after processing of the runs as described in the Section 2 and Section 2.5.4. Supplementary Table S5. Differential abundance test for comparison of the LPS + I1 vs. LPS groups. Proteins with CV ≤ 20.0% and quantified in at least 50% of the samples of each group were considered. Fold changes, resulting p-values, and Benjamini–Hochberg corrected p-values for each of the 6065 considered proteins are shown. Supplementary Figure S1. Effect of inulin on the lysosome pathway (KEGG: 04142) from RAW 264.7 LPS-induced inflammation model cells. Pathway diagrams overlayed with the measured protein fold change showing coherent cascades. Differentially expressed genes are represented with positive values in red. Supplementary Figure S2. Effect of inulin on the NF-κB signaling pathway showing integrated transcriptomics data. Genes overexpressed (in red) or underexpressed (green) according to the transcriptomic analysis are highlighted. Supplementary Figure S3. Effect of Inulin on the IL-17 signaling pathway (KEGG: 04657) from RAW 264.7 LPS-induced inflammation model cells. Pathway diagram, overlayed with the measured protein perturbation showing coherent cascades. Differentially expressed genes are represented with negative fold-change values in blue and positive values in red., Peer reviewed




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