BUSCADOR RECOLECTA

Digital.CSIC. Repositorio Institucional del CSIC

oai:digital.csic.es:10261/335980

32 pages. -- Figures and tables. -- Figure S1. Analysis of chalcone synthase structure for identification of substrate binding sites (PDB ID: 1CGK). -- Figure S2. Sequence alignment (Sievers et al., 2011) between Chalcone synthase (CHS) from Medicago sativa and Petunia hybrida. -- Figure S3. Measurement of intra- and extracellular concentrations of chalcone and naringenin in cells. -- Figure S4. Dependence of naringenin concentration and fluorescence intensity on the expression level of chs. -- Figure S5. Evaluation of naringenin production of mutants of each cluster with higher fluorescence than the parental strain in multi-well plates. -- Figure S6. Evaluation of beneficial mutants of four clusters in 3-mL test tubes which produced higher naringenin concentrations than the parental strain in multi-well plates. -- Figure S7. Evaluation of mutant libraries with beneficial mutations of each cluster in multi-well plates. -- Figure S8. Evaluation of beneficial mutants in 3-mL test tubes which produced higher naringenin concentrations than the parental strain in multi-well plates. -- Figure S9. Evaluation of enriched mutants through FACS-based screening. -- Figure S10. Accumulation of acetic acid in the parental strain and the CHSopt. Acetic acid production of the parental strain. -- Figure S11. Docking interactions of naringenin in CHS enzyme. -- Figure S12. Analysis of variants with the mutations of cluster 4. -- Figure S13. Effects of the repression of fabHDG expression on cell growth and naringenin production. -- Figure S14. Validation of the naringenin-production capacity of Parental-FRU3, CHSopt-FRU1, 2, 4, and 5 in flask cultures. -- Table S1. Previous studies on naringenin biosynthesis in similar cultivation conditions. -- Table S2. Strains and plasmids used in this study. -- Table S3. Oligonucleotides used in this study. -- Table S4. Information of T7 mutant promoters used in Fig S4. -- Table S5. Rational design of mutant libraries for four clusters in chs gene based on conservation of amino acids in chs and information of chalcone synthase homologs. -- Table S6. Sequence information of screened mutants from each cluster in chs through plate-based screening in Fig. S4. -- Table S7. Sequence information and naringenin production of screened variants from mutant libraries with combinations of beneficial mutations of each cluster through plate-based screening in Fig. S8. -- Table S8. Analysis of sequence information of screened variants through FACS-based screening in Fig S9. -- Table S9. Kinetic parameters of T132G and T132S/S133C in the CHS mutants. -- Table S10. Docking simulations summary. -- Table S11. 5'-UTR sequences and predicted expression levels of UTR variants used in this study., Peer reviewed