Dataset.

Glial-secreted Netrins regulate Robo1/Rac1-Cdc42 signaling threshold levels during Drosophila asymmetric neural stem and progenitor cell division [Dataset]

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311668
Digital.CSIC. Repositorio Institucional del CSIC
  • Torres Jurado, Ana de
  • Manzanero-Ortiz, Sandra
  • Carmena, Ana
Figures, original raw data, of the above paper., Peer reviewed
 
DOI: http://hdl.handle.net/10261/311668
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311668

HANDLE: http://hdl.handle.net/10261/311668
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311668
 
Ver en: http://hdl.handle.net/10261/311668
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311668

r-ISABIAL. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica y Sanitaria de Alicante
oai:fundanet.isabial.san.gva.es:p9065
Artículo científico (article). 2022

GLIAL-SECRETED NETRINS REGULATE ROBO1/RAC1-CDC42 SIGNALING THRESHOLD LEVELS DURING DROSOPHILA ASYMMETRIC NEURAL STEM/PROGENITOR CELL DIVISION

r-ISABIAL. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica y Sanitaria de Alicante
  • de Torres-Jurado, A
  • Manzanero-Ortiz, S
  • Carmena, A
Asymmetric stem cell division (ASCD) is a key mechanism in development, cancer, and stem cell biology. Drosophila neural stem cells, called neuroblasts (NBs), divide asymmetrically through intrinsic mechanisms. Here, we show that the extrinsic axon guidance cues Netrins, secreted by a glial niche surrounding larval brain neural stem cell lineages, regulate NB ASCD. Netrin-Frazzled/DCC signaling modulates, through Abelson kinase, Robo1 signaling threshold levels in Drosophila larval brain neural stem and progenitor cells of NBII lineages. Unbalanced Robo1 signaling levels induce ectopic NBs and progenitor cells due to failures in the ASCD process. Mechanistically, Robo1 signaling directly impinges on the intrinsic ASCD machinery, such as aPKC, Canoe/Afadin, and Numb, through the small GTPases Rac1 and Cdc42, which are required for the localization in mitotic NBs of Par-6, a Cdc42 physical partner and a core component of the Par (Par-6-aPKC-Par3/Bazooka) apical complex.



Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/278373
Artículo científico (article). 2022

GLIAL-SECRETED NETRINS REGULATE ROBO1/RAC1-CDC42 SIGNALING THRESHOLD LEVELS DURING DROSOPHILA ASYMMETRIC NEURAL STEM/PROGENITOR CELL DIVISION

Digital.CSIC. Repositorio Institucional del CSIC
  • Torres Jurado, Ana de
  • Manzanero-Ortiz, Sandra
  • Carmena, Ana
Asymmetric stem cell division (ASCD) is a key mechanism in development, cancer, and stem cell biology. Drosophila neural stem cells, called neuroblasts (NBs), divide asymmetrically through intrinsic mechanisms. Here, we show that the extrinsic axon guidance cues Netrins, secreted by a glial niche surrounding larval brain neural stem cell lineages, regulate NB ASCD. Netrin-Frazzled/DCC signaling modulates, through Abelson kinase, Robo1 signaling threshold levels in Drosophila larval brain neural stem and progenitor cells of NBII lineages. Unbalanced Robo1 signaling levels induce ectopic NBs and progenitor cells due to failures in the ASCD process. Mechanistically, Robo1 signaling directly impinges on the intrinsic ASCD machinery, such as aPKC, Canoe/Afadin, and Numb, through the small GTPases Rac1 and Cdc42, which are required for the localization in mitotic NBs of Par-6, a Cdc42 physical partner and a core component of the Par (Par-6-aPKC-Par3/Bazooka) apical complex., Our lab was supported by the Spanish grants from the Ministry of Economy and Competitiveness (BFU2015-64251); the Ministry of Science, Innovation, and Universities (PGC2018-097093-B-100); and by FEDER (European Regional Development Fund). A.T.-J. was supported by a predoctoral FPI fellowship associated with the Severo Ochoa (SO) Project (SEV-2013-0317), and S.M. was supported by a predoctoral FPI fellowship associated with the BFU2015-64251 grant. The ‘‘Instituto de Neurociencias’’ in Alicante is a ‘‘Severo Ochoa Center of Excellence in R&D.’’, Peer reviewed




Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311668
Dataset. 2022

GLIAL-SECRETED NETRINS REGULATE ROBO1/RAC1-CDC42 SIGNALING THRESHOLD LEVELS DURING DROSOPHILA ASYMMETRIC NEURAL STEM AND PROGENITOR CELL DIVISION [DATASET]

Digital.CSIC. Repositorio Institucional del CSIC
  • Torres Jurado, Ana de
  • Manzanero-Ortiz, Sandra
  • Carmena, Ana
Figures, original raw data, of the above paper., Peer reviewed




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