BUSCADOR RECOLECTA

Digital.CSIC. Repositorio Institucional del CSIC

oai:digital.csic.es:10261/331755

S3 Fig. EDEM depletion mitigates ER stress. (A) Representative confocal images of WT(EV) control or indicated RNAi depleted young adults carrying the Phsp-4::GFP transgene under non ER-stress conditions (upper panel), treatment with 5 μg/ml tunicamycin for 12h (middle panel), and heat shock, and 5 μM thapsigargin treatment (lower panel). TM-tunicamycin, HS-heat shock, TG-thapsigargin. The worms were outlined for better visualization. Images were obtained using the same confocal settings. Exposure adjustments were uniformly applied. Scale bar: 50 μm. (B) Quantitative RT-PCR measurements of Phsp-4::mRNA levels in indicated strains under non stress and treatment with 5 μg/ml tunicamycin (n = 3 independent experiments). (C) Quantitative RT-PCR measurements of Phsp-4::mRNA levels in indicated strains under non stress and heat stress treatment (n = 3 independent experiments). In (B) and (C) quantifications were normalized relative to WT non stress conditions. ****P<0.0001, ns, not significant. Quantifications were normalized relative to WT non stress conditions. (D) Representative confocal images of WT control and edem-2 mutants carrying the PC12C8.1::GFP transgene under non ER-stress conditions. The left panels show fluorescence images and the right panels DIC images., Peer reviewed