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Digital.CSIC. Repositorio Institucional del CSIC

oai:digital.csic.es:10261/331042

Figure S1. Presence/absence of genes coding for potential PAMPs and CWDE across well-annotated genomes of Xylella species. The circles from in to out represent elf18, csp20, chiA, pglA, prtA, lesA and wzy. GBK and fasta files of both the amino acid and nucleotide sequences of the represented genomes were downloaded from the NCBI database. Blast searches were performed using the sequence of X. fastidiosa subsp. fastidiosa Temecula as a template. Blasts using Koala were performed to check for presence absence of the LPS, EPS, and PGN biosynthesis pathway, as well as secretion systems. Table S1. Gene expression to microbial stimuli in xylem tissues. For creating this list, two published datasets were crossed: Wendrich et al., 2020 (https://bioit3.irc.ugent.be/plant-sc-atlas/) and Fröscher et al., 2020. Table S2. Potential PRRs and NLRs in genomes of plants susceptible to X. fastidiosa based on annotation searches. GBK files from each of the host plants' genomes were downloaded from the NCBI database. Then, for retrieving the number of potential PRR and NLR genes we have searched for known protein keywords related to these two families of genes using python custom scripts to parse the GBK files using the Biopython library and specifically SeqIO function. Based on gene name or TAIR locustag (in the case of A. thaliana) we dropped the duplicate genes (splicing products)., Peer reviewed