Dataset.

Additional file 1 of Overexpression of wild type RRAS2, without oncogenic mutations, drives chronic lymphocytic leukemia [Dataset]

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/329845
Digital.CSIC. Repositorio Institucional del CSIC
  • Hortal, Alejandro
  • Oeste, Clara L.
  • Cifuentes, Claudia
  • Alcoceba, Miguel
  • Fernández-Pisonero, Isabel
  • Clavaín, Laura
  • Tercero, Rut
  • Mendoza, Pilar
  • Domínguez, Verónica
  • García-Flores, Marta
  • Pintado, Belén
  • Abia, David
  • García-Macías, Carmen
  • Navarro-Bailón, Almudena
  • Bustelo, Xosé R.
  • González, Marcos
  • Alarcón, Balbino
Additional file 1: Figure S1. a, Relative mRNA expression of RRAS2 in different types of leukemia. Data comes from (Haferlach et al., 2010) and has been retrieved from www.oncomine.org . b, Schematic representation of the overexpression cassette inserted into the Rosa26 locus. c, Relative expression of RRAS2 measured by RT-qPCR in different organs of Rosa26-RRAS2fl/flxSox2-Cre (Sox2-Cre+) mice compared to that of WT C57BL/6 J Control mice using 18S as the reference gene. All expression numbers were normalized to those of liver from WT Control mice (mean = 1). Data show relative expression of RRAS2 in the indicated organs in n = 3–4 8 month-old independent mice. d, Quantification of spleen weight from control and 6 month-old Sox2-Cre + mice. Data shown correspond to four control mice and eleven Sox2-Cre mice. Two-tailed unpaired t-test with Welch’s correction. e, Two-parameter flow cytometry of the expression of CD5 and IgM in B cells in the spleen of 6 month-old control and Sox2-Cre + mice. f, Quantification of the number of CD5 + IgM+ B cells in the spleens and bone marrow of 6 month-old control and Sox2-Cre + mice. Data correspond to triplicate measurements of one control and three Sox2-Cre mice. Unpaired t-test with Welch’s correction. g, Quantification of the serum IgM concentration in the blood of 35–40 wk-old control (n = 3) and mb1-Cre (n = 8) mice by ELISA. Unpaired t-test with Welch’s correction. h, Representative images from Giemsa stainings of blood smears of 36 wk-old control and mb1-Cre mice. i, Two-parameter flow cytometry of the forward scatter and CD5 expression in CD19+ cells in the blood of 16 wk-old mb1-Cre mice. The gated population represents large cells. j, Two-parameter flow cytometry of CD5 expression and BrdU incorporation in CD19+ cells in the blood of 16 wk-old mb1-Cre mice. k, Quantification of the percentage of CD19+ cells that are CD5+ blasts and of the CD19+ CD5+ cells that have incorporated BrdU., Peer reviewed
 
DOI: http://hdl.handle.net/10261/329845
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/329845

HANDLE: http://hdl.handle.net/10261/329845
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/329845
 
Ver en: http://hdl.handle.net/10261/329845
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/329845

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/329845
Dataset. 2022

ADDITIONAL FILE 1 OF OVEREXPRESSION OF WILD TYPE RRAS2, WITHOUT ONCOGENIC MUTATIONS, DRIVES CHRONIC LYMPHOCYTIC LEUKEMIA [DATASET]

Digital.CSIC. Repositorio Institucional del CSIC
  • Hortal, Alejandro
  • Oeste, Clara L.
  • Cifuentes, Claudia
  • Alcoceba, Miguel
  • Fernández-Pisonero, Isabel
  • Clavaín, Laura
  • Tercero, Rut
  • Mendoza, Pilar
  • Domínguez, Verónica
  • García-Flores, Marta
  • Pintado, Belén
  • Abia, David
  • García-Macías, Carmen
  • Navarro-Bailón, Almudena
  • Bustelo, Xosé R.
  • González, Marcos
  • Alarcón, Balbino
Additional file 1: Figure S1. a, Relative mRNA expression of RRAS2 in different types of leukemia. Data comes from (Haferlach et al., 2010) and has been retrieved from www.oncomine.org . b, Schematic representation of the overexpression cassette inserted into the Rosa26 locus. c, Relative expression of RRAS2 measured by RT-qPCR in different organs of Rosa26-RRAS2fl/flxSox2-Cre (Sox2-Cre+) mice compared to that of WT C57BL/6 J Control mice using 18S as the reference gene. All expression numbers were normalized to those of liver from WT Control mice (mean = 1). Data show relative expression of RRAS2 in the indicated organs in n = 3–4 8 month-old independent mice. d, Quantification of spleen weight from control and 6 month-old Sox2-Cre + mice. Data shown correspond to four control mice and eleven Sox2-Cre mice. Two-tailed unpaired t-test with Welch’s correction. e, Two-parameter flow cytometry of the expression of CD5 and IgM in B cells in the spleen of 6 month-old control and Sox2-Cre + mice. f, Quantification of the number of CD5 + IgM+ B cells in the spleens and bone marrow of 6 month-old control and Sox2-Cre + mice. Data correspond to triplicate measurements of one control and three Sox2-Cre mice. Unpaired t-test with Welch’s correction. g, Quantification of the serum IgM concentration in the blood of 35–40 wk-old control (n = 3) and mb1-Cre (n = 8) mice by ELISA. Unpaired t-test with Welch’s correction. h, Representative images from Giemsa stainings of blood smears of 36 wk-old control and mb1-Cre mice. i, Two-parameter flow cytometry of the forward scatter and CD5 expression in CD19+ cells in the blood of 16 wk-old mb1-Cre mice. The gated population represents large cells. j, Two-parameter flow cytometry of CD5 expression and BrdU incorporation in CD19+ cells in the blood of 16 wk-old mb1-Cre mice. k, Quantification of the percentage of CD19+ cells that are CD5+ blasts and of the CD19+ CD5+ cells that have incorporated BrdU., Peer reviewed




1106