Publicación Artículo científico (article).

A multifaceted small RNA modulates gene expression upon glucose limitation in Staphylococcus aureus

Digital.CSIC. Repositorio Institucional del CSIC
Digital.CSIC. Repositorio Institucional del CSIC
  • Bronesky, Delphine
  • Desgranges, Emma
  • Corvaglia, Anna
  • François, Patrice
  • Caballero Sánchez, Carlos José
  • Prado, Laura
  • Toledo-Arana, Alejandro
  • Lasa, Íñigo
  • Moreau, Karan
  • Vandenesch, François
  • Marzi, Stefano
  • Romby, Pascale
  • Caldelari, Isabelle
Pathogenic bacteria must rapidly adapt to ever‐changing environmental signals resulting in metabolism remodeling. The carbon catabolite repression, mediated by the catabolite control protein A (CcpA), is used to express genes involved in utilization and metabolism of the preferred carbon source. Here, we have identified RsaI as a CcpA‐repressed small non‐coding RNA that is inhibited by high glucose concentrations. When glucose is consumed, RsaI represses translation initiation of mRNAs encoding a permease of glucose uptake and the FN3K enzyme that protects proteins against damage caused by high glucose concentrations. RsaI also binds to the 3′ untranslated region of icaR mRNA encoding the transcriptional repressor of exopolysaccharide production and to sRNAs induced by the uptake of glucose‐6 phosphate or nitric oxide. Furthermore, RsaI expression is accompanied by a decreased transcription of genes involved in carbon catabolism pathway and an activation of genes involved in energy production, fermentation, and nitric oxide detoxification. This multifaceted RNA can be considered as a metabolic signature when glucose becomes scarce and growth is arrested., This work was supported by the Centre National de la Recherche Scientifique (CNRS) to [P.R.] and by the Agence Nationale de la Recherche (ANR, grant ANR‐16‐CE11‐0007‐01, RIBOSTAPH, to [P.R.]). It has also been published under the framework of the LABEX: ANR‐10‐LABX‐0036 NETRNA to [P.R.], a funding from the state managed by the French National Research Agency as part of the investments for the future program. The work is financed by a “Projet international de coopération scientifique” (PICS) No. PICS07507 between France and Spain to [I.C.]. D. Bronesky was supported by Fondation pour la Recherche Médicale (FDT20160435025). A. T‐A is financed by the Spanish Ministry of Economy and Competitiveness (BFU2014‐56698‐P) and the European Research Council Consolidator Grant (646869‐ReguloBac‐3UTR)., Peer reviewed

Digital.CSIC. Repositorio Institucional del CSIC

Digital.CSIC. Repositorio Institucional del CSIC
Ver en:
Digital.CSIC. Repositorio Institucional del CSIC