Dataset.
Interactions between the Parasite Philasterides dicentrarchi and the Immune System of the Turbot Scophthalmus maximus. a Transcriptomic Analysis - Supplementary Materials
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/360751
Digital.CSIC. Repositorio Institucional del CSIC
- Valle, Alejandra
- Leiro, José Manuel
- Pereiro, Patricia
- Figueras Huerta, Antonio
- Novoa, Beatriz
- Dirks, Ron P. H.
- Lamas, Jesús
4 files, Supplementary material for the article http://dx.doi.org/10.3390/biology9100337, Doc file: Figure S1: Number of turbot cells and ciliates in the peritoneal cavity; Figure S2: Micrographs of ciliates at different stages of infection; Figure S3: qPCR validation of RNA-seq findings; Table S1: Sample information; Table S2: Primer sequences used in the qPCR analysis.-- File S1: Excel file showing the contigs that were DE in P. dicentrarchi at 1, 2, 4 hpi, the fold change, p-value, and the gene names.-- File S2: Excel file showing the contigs that were DE in turbot peritoneal cells at 1, 2, 4 hpi, the fold change, p-value, and the gene names.-- File S3: Excel file showing the contigs that were DE in turbot peritoneal cells at 12 and 48 hpi, the fold change, p-value, and the gene names, Peer reviewed
DOI: http://hdl.handle.net/10261/360751
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/360751
HANDLE: http://hdl.handle.net/10261/360751
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/360751
Ver en: http://hdl.handle.net/10261/360751
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/360751
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1 Documentos relacionados
1 Documentos relacionados
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/307872
Artículo científico (article). 2020
INTERACTIONS BETWEEN THE PARASITE PHILASTERIDES DICENTRARCHI AND THE IMMUNE SYSTEM OF THE TURBOT SCOPHTHALMUS MAXIMUS. A TRANSCRIPTOMIC ANALYSIS
Digital.CSIC. Repositorio Institucional del CSIC
- Valle, Alejandra
- Leiro, José Manuel
- Pereiro, Patricia
- Figueras Huerta, Antonio
- Novoa, Beatriz
- Dirks, Ron P. H.
- Lamas, Jesús
26 pages, 9 tables, 2 figures.-- This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license, The present study analyses the interactions between Philasterides dicentrarchi (a ciliate parasite that causes high mortalities in cultured flatfish) and the peritoneal cells of the turbot Scophthalmus maximus during an experimental infection. The transcriptomic response was evaluated in the parasites and in the fish peritoneal cells, at 1, 2 and 4 h post-infection (hpi) in turbot injected intraperitoneally (ip) with 107 ciliates and at 12 and 48 hpi in turbot injected ip with 105 ciliates. Numerous genes were differentially expressed (DE) in P. dicentrarchi, relative to their expression in control ciliates (0 hpi): 407 (369 were up-regulated) at 1 hpi, 769 (415 were up-regulated) at 2 hpi and 507 (119 were up-regulated) at 4 hpi. Gene ontology (GO) analysis of the DE genes showed that the most representative categories of biological processes affected at 1, 2 and 4 hpi were biosynthetic processes, catabolic processes, biogenesis, proteolysis and transmembrane transport. Twelve genes of the ABC transporter family and eight genes of the leishmanolysin family were DE at 1, 2 and 4 hpi. Most of these genes were strongly up-regulated (UR), suggesting that they are involved in P. dicentrarchi infection. A third group of UR genes included several genes related to ribosome biogenesis, DNA transcription and RNA translation. However, expression of tubulins and tubulin associated proteins, such as kinesins or dyneins, which play key roles in ciliate division and movement, was down-regulated (DR). Similarly, genes that coded for lysosomal proteins or that participate in the cell cycle mitotic control, glycolysis, the Krebs cycle and/or in the electron transport chain were also DR. The transcriptomic analysis also revealed that in contrast to many parasites, which passively evade the host immune system, P. dicentrarchi strongly stimulated turbot peritoneal cells. Many genes related to inflammation were DE in peritoneal cells at 1, 2 and 4 hpi. However, the response was much lower at 12 hpi and almost disappeared completely at 48 hpi in fish that were able to kill P. dicentrarchi during the first few hpi. The genes that were DE at 1, 2 and 4 hpi were mainly related to the apoptotic process, the immune response, the Fc-epsilon receptor signalling pathway, the innate immune response, cell adhesion, cell surface receptors, the NF-kappaB signalling pathway and the MAPK cascade. Expression of toll-like receptors 2, 5 and 13 and of several components of NF-κB, MAPK and JAK/STAT signalling pathways was UR in the turbot peritoneal cells. Genes expressing chemokines and chemokine receptors, genes involved in prostaglandin and leukotriene synthesis, prostaglandins, leukotriene receptors, proinflammatory cytokines and genes involved in apoptosis were strongly UR during the first four hours of infection. However, expression of anti-inflammatory cytokines such as Il-10 and lipoxygenases with anti-inflammatory activity (i.e., arachidonate 15-lipoxygenase) were only UR at 12 and/or 48 hpi, indicating an anti-inflammatory state in these groups of fish. In conclusion, the present study shows the regulation of several genes in P. dicentrarchi during the early stages of infection, some of which probably play important roles in this process. The infection induced a potent acute inflammatory response, and many inflammatory genes were regulated in peritoneal cells, showing that the turbot uses all the protective mechanisms it has available to prevent the entry of the parasite., This study was financially supported by grant AGL2017-83577-R awarded by the Ministerio de Economía y Competitividad (Spain) and Fondo Europeo de Desarrollo Regional -FEDER- (European Union), by grant ED431C2017/31 from the Xunta de Galicia (Spain), and by PARAFISHCONTROL project, via funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement no. 634429., Peer reviewed
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1 Versiones
1 Versiones
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/360751
Dataset. 2020
INTERACTIONS BETWEEN THE PARASITE PHILASTERIDES DICENTRARCHI AND THE IMMUNE SYSTEM OF THE TURBOT SCOPHTHALMUS MAXIMUS. A TRANSCRIPTOMIC ANALYSIS - SUPPLEMENTARY MATERIALS
Digital.CSIC. Repositorio Institucional del CSIC
- Valle, Alejandra
- Leiro, José Manuel
- Pereiro, Patricia
- Figueras Huerta, Antonio
- Novoa, Beatriz
- Dirks, Ron P. H.
- Lamas, Jesús
4 files, Supplementary material for the article http://dx.doi.org/10.3390/biology9100337, Doc file: Figure S1: Number of turbot cells and ciliates in the peritoneal cavity; Figure S2: Micrographs of ciliates at different stages of infection; Figure S3: qPCR validation of RNA-seq findings; Table S1: Sample information; Table S2: Primer sequences used in the qPCR analysis.-- File S1: Excel file showing the contigs that were DE in P. dicentrarchi at 1, 2, 4 hpi, the fold change, p-value, and the gene names.-- File S2: Excel file showing the contigs that were DE in turbot peritoneal cells at 1, 2, 4 hpi, the fold change, p-value, and the gene names.-- File S3: Excel file showing the contigs that were DE in turbot peritoneal cells at 12 and 48 hpi, the fold change, p-value, and the gene names, Peer reviewed
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