BUSCADOR RECOLECTA

Digital.CSIC. Repositorio Institucional del CSIC

oai:digital.csic.es:10261/310824

(A) Representative pictures under white light of infected and non-treated N. benthamiana plants at 11 days post-inoculation. White bar = 4 cm. (B) Representative pictures under white light of upper non-inoculated leaves, at 40 days post-inoculation (dpi), of cassava plants inoculated with the indicated viruses. White arrows indicate the presence of leaf chlorosis induced by UCBSV in cassava. White bar = 4 cm. (C) Representative pictures under white light of stems, at 90 dpi, of cassava plants inoculated with the indicated viruses. Black arrows indicate the presence of brown streaks induced by UCBSV in cassava. (D) Chromatograms of Sanger sequencing results of the DNA fragment of interest amplified by RT-PCR. RNA samples deriving from upper non-inoculated leaves of the indicated cassava plant inoculated with UCBSV-HAM1N35A were used as template. Leaves for RNA preparation were harvested at both 90 and 120 days post-infection (dpi). Coloured circles surround both the original and the naturally introduced mutation. (E) Cropped polyacrylamide gel stained with coomasie blue that shows that equivalent amounts of the indicated proteins were used for the experiment in Fig 4E. 1:20 dilutions (10, 20 and 40 nanograms, correspondingly) were used for the measurement of ITPase activity., Peer reviewed