Publicación Artículo científico (article).

Differential evolution in 3′UTRs leads to specific gene expression in Staphylococcus

Digital.CSIC. Repositorio Institucional del CSIC
Digital.CSIC. Repositorio Institucional del CSIC
  • Menéndez Gil, Pilar
  • Caballero Sánchez, Carlos José
  • Catalán Moreno, Arancha
  • Irurzun, Naiara
  • Barrio-Hernandez, Iñigo
  • Caldelari, Isabelle
  • Toledo-Arana, Alejandro
The evolution of gene expression regulation has contributed to species differentiation. The 3′ untranslated regions (3′UTRs) of mRNAs include regulatory elements that modulate gene expression; however, our knowledge of their implications in the divergence of bacterial species is currently limited. In this study, we performed genome-wide comparative analyses of mRNAs encoding orthologous proteins from the genus Staphylococcus and found that mRNA conservation was lost mostly downstream of the coding sequence (CDS), indicating the presence of high sequence diversity in the 3′UTRs of orthologous genes. Transcriptomic mapping of different staphylococcal species confirmed that 3′UTRs were also variable in length. We constructed chimeric mRNAs carrying the 3′UTR of orthologous genes and demonstrated that 3′UTR sequence variations affect protein production. This suggested that species-specific functional 3′UTRs might be specifically selected during evolution. 3′UTR variations may occur through different processes, including gene rearrangements, local nucleotide changes, and the transposition of insertion sequences. By extending the conservation analyses to specific 3′UTRs, as well as the entire set of Escherichia coli and Bacillus subtilis mRNAs, we showed that 3′UTR variability is widespread in bacteria. In summary, our work unveils an evolutionary bias within 3′UTRs that results in species-specific non-coding sequences that may contribute to bacterial diversity., A.T.-A. was supported by the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program [ERC-CoG-2014-646869] and the Spanish Ministry of Economy and Competitiveness [BFU2014-56698-P] grants; C.J.C. was supported by a predoctoral contract from the Public University of Navarre; I.C. was supported by recurrent funding from the Centre National de la Recherche Scientifique (CNRS) and by a PICS program (‘Projet International de Coopération Scientifique’, No. PICS07507, CNRS) between France and Spain. Funding for open access charge: European Research Council (ERC), European Union's Horizon 2020 research and innovation program [ERC-CoG-2014-646869].

Digital.CSIC. Repositorio Institucional del CSIC

Digital.CSIC. Repositorio Institucional del CSIC
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Digital.CSIC. Repositorio Institucional del CSIC