Dataset.
Using Electrochemical Immunoassay in a Novel Microtiter Plate to Detect Surface Markers of Preeclampsia on Urinary Extracellular Vesicles [Dataset]
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/337665
Digital.CSIC. Repositorio Institucional del CSIC
- Lee, Seonhwa
- González-Suárez, Alan M.
- Huang, XuHai
- Calvo-Lozano, Olalla
- Suvakov, Sonja
- Lechuga, Laura M.
- Garovic, Vesna D.
- Stybayeva, Gulnaz
- Revzin, Alexander
8 pages. -- Figure S1. Schematic diagram describing integration of the MUA-functionalized electrode array with a microwell layer. -- Figure S2. Electrochemical characterization of a microtiter plate. -- Figure S3. Nanoparticle-enabled electrochemical immunoassay for detection of podocin and nephrin. -- Figure S4. Characterization of urinary EVs. -- Figure S5. Using SPR to characterize EV capture and immunoprobe binding. -- Figure S6. Representative SEM images of EVs captured on an electrode surface functionalized with anti-CD63 Abs. -- Figure S7. SPR analysis of clinical urine samples., Extracellular vesicles (EVs) are lipid bilayer nanovesicles secreted by cells. EVs contain biological information related to parental cells and provide biomarkers for disease diagnosis. We have previously shown that the levels of podocin and nephrin expression on urinary EVs may be used to diagnose renal injury associated with preeclampsia. This paper describes a nanoparticle-enabled immunoassay integrated with an electrochemical plate for quantifying podocin and nephrin expression in urinary EVs. The strategy entailed capturing EVs on an electrode surface and then labeling EVs with gold nanoparticles that are both functionalized with antibodies for target specificity and impregnated with redox-active metal ions for electrochemical detection. These immunoprobes produced an electrochemical redox signal proportional to the expression level of EV surface markers. Electrochemical immunoassays were carried out in a novel microtiter plate that contained 16 wells with working electrodes connected to onboard counter/reference electrodes via capillary valves. Upon validation with recombinant proteins, a microtiter plate was used for analysis of urinary EVs from healthy and preeclamptic pregnant women. This analysis revealed a higher podocin to nephrin ratio for preeclamptic women compared to healthy controls (4.31 vs 1.69) suggesting that this ratio may be used for disease diagnosis., Peer reviewed
DOI: http://hdl.handle.net/10261/337665
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/337665
HANDLE: http://hdl.handle.net/10261/337665
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/337665
Ver en: http://hdl.handle.net/10261/337665
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/337665
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1 Documentos relacionados
1 Documentos relacionados
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/337083
Artículo científico (article). 2023
USING ELECTROCHEMICAL IMMUNOASSAY IN A NOVEL MICROTITER PLATE TO DETECT SURFACE MARKERS OF PREECLAMPSIA ON URINARY EXTRACELLULAR VESICLES
Digital.CSIC. Repositorio Institucional del CSIC
- Lee, Seonhwa
- González-Suárez, Alan M.
- Huang, XuHai
- Calvo-Lozano, Olalla
- Suvakov, Sonja
- Lechuga, Laura M.
- Garovic, Vesna D.
- Stybayeva, Gulnaz
- Revzin, Alexander
Extracellular vesicles (EVs) are lipid bilayer nanovesicles secreted by cells. EVs contain biological information related to parental cells and provide biomarkers for disease diagnosis. We have previously shown that the levels of podocin and nephrin expression on urinary EVs may be used to diagnose renal injury associated with preeclampsia. This paper describes a nanoparticle-enabled immunoassay integrated with an electrochemical plate for quantifying podocin and nephrin expression in urinary EVs. The strategy entailed capturing EVs on an electrode surface and then labeling EVs with gold nanoparticles that are both functionalized with antibodies for target specificity and impregnated with redox-active metal ions for electrochemical detection. These immunoprobes produced an electrochemical redox signal proportional to the expression level of EV surface markers. Electrochemical immunoassays were carried out in a novel microtiter plate that contained 16 wells with working electrodes connected to onboard counter/reference electrodes via capillary valves. Upon validation with recombinant proteins, a microtiter plate was used for analysis of urinary EVs from healthy and preeclamptic pregnant women. This analysis revealed a higher podocin to nephrin ratio for preeclamptic women compared to healthy controls (4.31 vs 1.69) suggesting that this ratio may be used for disease diagnosis., This work was funded by grants from NIH (HD 96993 and P30DK084567)., Peer reviewed
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1 Versiones
1 Versiones
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/337665
Dataset. 2022
USING ELECTROCHEMICAL IMMUNOASSAY IN A NOVEL MICROTITER PLATE TO DETECT SURFACE MARKERS OF PREECLAMPSIA ON URINARY EXTRACELLULAR VESICLES [DATASET]
Digital.CSIC. Repositorio Institucional del CSIC
- Lee, Seonhwa
- González-Suárez, Alan M.
- Huang, XuHai
- Calvo-Lozano, Olalla
- Suvakov, Sonja
- Lechuga, Laura M.
- Garovic, Vesna D.
- Stybayeva, Gulnaz
- Revzin, Alexander
8 pages. -- Figure S1. Schematic diagram describing integration of the MUA-functionalized electrode array with a microwell layer. -- Figure S2. Electrochemical characterization of a microtiter plate. -- Figure S3. Nanoparticle-enabled electrochemical immunoassay for detection of podocin and nephrin. -- Figure S4. Characterization of urinary EVs. -- Figure S5. Using SPR to characterize EV capture and immunoprobe binding. -- Figure S6. Representative SEM images of EVs captured on an electrode surface functionalized with anti-CD63 Abs. -- Figure S7. SPR analysis of clinical urine samples., Extracellular vesicles (EVs) are lipid bilayer nanovesicles secreted by cells. EVs contain biological information related to parental cells and provide biomarkers for disease diagnosis. We have previously shown that the levels of podocin and nephrin expression on urinary EVs may be used to diagnose renal injury associated with preeclampsia. This paper describes a nanoparticle-enabled immunoassay integrated with an electrochemical plate for quantifying podocin and nephrin expression in urinary EVs. The strategy entailed capturing EVs on an electrode surface and then labeling EVs with gold nanoparticles that are both functionalized with antibodies for target specificity and impregnated with redox-active metal ions for electrochemical detection. These immunoprobes produced an electrochemical redox signal proportional to the expression level of EV surface markers. Electrochemical immunoassays were carried out in a novel microtiter plate that contained 16 wells with working electrodes connected to onboard counter/reference electrodes via capillary valves. Upon validation with recombinant proteins, a microtiter plate was used for analysis of urinary EVs from healthy and preeclamptic pregnant women. This analysis revealed a higher podocin to nephrin ratio for preeclamptic women compared to healthy controls (4.31 vs 1.69) suggesting that this ratio may be used for disease diagnosis., Peer reviewed
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