BUSCADOR RECOLECTA

Digital.CSIC. Repositorio Institucional del CSIC

oai:digital.csic.es:10261/331746

S1 Fig. Immunoblot analysis of Pedem::GFP expression in young animals. (A) Quantitative real-time PCR (qPCR) of edem-3 mRNA. WT and edem-3 mutant worms treated with empty vector or edem-3 RNAi; expression were normalized to that of cdc-42 and pmp-3. (B) Total protein lysates derived from WT Pedem::GFP transgenic animals subjected to the indicated treatment were separated by SDS-PAGE and immunoblotted with anti-GFP polyclonal antisera; tubulin was used as loading control. NS- non-treated, TM- tunicamycin, HS-heat stress, OS- osmotic stress. The histograms show the densitometry values of Pedem::GFP bands normalized to the value of of NS condition (n=3 ± SEM, t test), *P<0.05; **P<0.01; ***P<0.001; ns, not significant. (C) RNAi downregulation of edem triggered accumulation of CPL-1* in intestinal cells. To overrule a significant contribution of autofluorescent stress granules to the GFP fluorescence, images captured with Diode laser were included. Scale bar: 20 μm., Peer reviewed