Dataset.
Evaluation of the effects of cold atmospheric plasma and plasma-treated liquids in cancer cell cultures [Dataset]
UPCommons. Portal del coneixement obert de la UPC
oai:upcommons.upc.edu:2117/330395
UPCommons. Portal del coneixement obert de la UPC
- Tornin Cavielles, Juan|||0000-0002-7676-3958
- Labay, Cédric Pierre|||0000-0001-5147-5100
- Tampieri, Francesco|||0000-0003-1474-867X
- Ginebra Molins, Maria Pau|||0000-0002-4700-5621
- Canal Barnils, Cristina|||0000-0002-3039-7462
This dataset is related to the protocol developed by the authors on the evaluation of the effects of cold atmospheric plasma and plasma-treated liquids in cancer cell cultures. More specifically, the data correspond to the anticipated results presented in the paper submitted to Nature Protocols, The present dataset corresponds to the anticipated results that can be obtained following the protocol described in the article. In particular, dataset corresponding to Figure 8 presents the absorbance values from the WST-1 cytotoxicity assay as a function of the well size where the experiment is performed. Data from Figure 9b show absorbance values from the WST-1 cytotoxicity assay as a function of the treatment time. Data from figure 9c-e show the absorbance or fluorescence values obtained in the experiments to quantify RONS, together with the values from the calibration curves. Data fromfigure 10 a provide the list and values of mRNA fold induction of the genes analyzed, and data from figure 10c show the pixel intensity obtained for the phospho-kinase antibody array.
DOI: http://hdl.handle.net/2117/360424, https://dx.doi.org/10.5821/data-2117-330395-1
UPCommons. Portal del coneixement obert de la UPC
oai:upcommons.upc.edu:2117/330395
HANDLE: http://hdl.handle.net/2117/360424, https://dx.doi.org/10.5821/data-2117-330395-1
UPCommons. Portal del coneixement obert de la UPC
oai:upcommons.upc.edu:2117/330395
Ver en: http://hdl.handle.net/2117/360424, https://dx.doi.org/10.5821/data-2117-330395-1
UPCommons. Portal del coneixement obert de la UPC
oai:upcommons.upc.edu:2117/330395
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1 Documentos relacionados
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r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
oai:fundanet.fsjd.org:p19598
Artículo científico (article). 2021
EVALUATION OF THE EFFECTS OF COLD ATMOSPHERIC PLASMA AND PLASMA-TREATED LIQUIDS IN CANCER CELL CULTURES.
r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
- Tornin J
- Labay C
- Tampieri F
- Ginebra MP
- Canal C
Cold atmospheric plasma (CAP) is a potential anticancer therapy. CAP has cytotoxic effects when applied either directly to cancer cell cultures or indirectly through plasma-conditioned liquids. This protocol describes how to treat adherent cultures of human cancer cell lines with CAP or plasma-conditioned medium and determine cell viability following treatment. The protocol also includes details on how to quantify the reactive oxygen and nitrogen species present in medium following CAP treatment, using chemical probes using UV-visible or fluorescence spectroscopy. CAP treatment takes ~30 min, and 3 h are required to complete quantification of reactive oxygen and nitrogen species. By providing a standardized protocol for evaluation of the effects of CAP and plasma-conditioned medium, we hope to facilitate the comparison and interpretation of results seen across different laboratories.
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1 Versiones
1 Versiones
UPCommons. Portal del coneixement obert de la UPC
oai:upcommons.upc.edu:2117/330395
Dataset. 2020
EVALUATION OF THE EFFECTS OF COLD ATMOSPHERIC PLASMA AND PLASMA-TREATED LIQUIDS IN CANCER CELL CULTURES [DATASET]
UPCommons. Portal del coneixement obert de la UPC
- Tornin Cavielles, Juan|||0000-0002-7676-3958
- Labay, Cédric Pierre|||0000-0001-5147-5100
- Tampieri, Francesco|||0000-0003-1474-867X
- Ginebra Molins, Maria Pau|||0000-0002-4700-5621
- Canal Barnils, Cristina|||0000-0002-3039-7462
This dataset is related to the protocol developed by the authors on the evaluation of the effects of cold atmospheric plasma and plasma-treated liquids in cancer cell cultures. More specifically, the data correspond to the anticipated results presented in the paper submitted to Nature Protocols, The present dataset corresponds to the anticipated results that can be obtained following the protocol described in the article. In particular, dataset corresponding to Figure 8 presents the absorbance values from the WST-1 cytotoxicity assay as a function of the well size where the experiment is performed. Data from Figure 9b show absorbance values from the WST-1 cytotoxicity assay as a function of the treatment time. Data from figure 9c-e show the absorbance or fluorescence values obtained in the experiments to quantify RONS, together with the values from the calibration curves. Data fromfigure 10 a provide the list and values of mRNA fold induction of the genes analyzed, and data from figure 10c show the pixel intensity obtained for the phospho-kinase antibody array.
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