Data associated with the study called "Selectively targeting tumorigenicity in osteosarcoma". Each file corresponds to the raw data of one of the figures of the paper, 1

Data associated with the study called "Selectively targeting tumorigenicity in osteosarcoma". Each file corresponds to the raw data of one of the figures of the paper

These data are associated to the paper with the same title of the authors, and are structured according to the figures in the paper: http://hdl.handle.net/2117/335727, The use of Cold Atmospheric Plasma (CAP) in oncology is being recently studied as a novel potential anti-cancer therapy. However, the beneficial effects of CAP for treating osteosarcoma have mostly been demonstrated in 2-dimensional cultures of cells, which do not mimic the complexity of the 3-dimensional (3D) bone microenvironment. In order to evaluate the effects of CAP in a relevant context of the human disease, we developed a 3D tissue-engineered model of osteosarcoma using a bone-like scaffold made of collagen type I and hydroxyapatite nanoparticles. Human osteosarcoma cells cultured within the scaffold showed a high capacity to infiltrate and proliferate and to exhibit osteomimicry in vitro. As expected, we observed significantly different functional behaviors between monolayer and 3D cultures when treated with Cold Plasma-Activated Ringer’s Solution (PAR). Our data reveal that the 3D environment not only protects cells from PAR-induced lethality by scavenging and diminishing the amount of reactive oxygen and nitrogen species generated by CAP, but also favours the stemness phenotype of osteosarcoma cells. This is the first study that demonstrates the negative effect of PAR on cancer stem-like cell subpopulations in a 3D biomimetic model of cancer. These findings will allow to suitably re-focus research on plasma-based therapies in future

This dataset is related to the protocol developed by the authors on the evaluation of the effects of cold atmospheric plasma and plasma-treated liquids in cancer cell cultures. More specifically, the data correspond to the anticipated results presented in the paper submitted to Nature Protocols, The present dataset corresponds to the anticipated results that can be obtained following the protocol described in the article. In particular, dataset corresponding to Figure 8 presents the absorbance values from the WST-1 cytotoxicity assay as a function of the well size where the experiment is performed. Data from Figure 9b show absorbance values from the WST-1 cytotoxicity assay as a function of the treatment time. Data from figure 9c-e show the absorbance or fluorescence values obtained in the experiments to quantify RONS, together with the values from the calibration curves. Data fromfigure 10 a provide the list and values of mRNA fold induction of the genes analyzed, and data from figure 10c show the pixel intensity obtained for the phospho-kinase antibody array.

This dataset is related to the protocol developed by the authors on the evaluation of the effects of cold atmospheric plasma and plasma-treated liquids in cancer cell cultures. More specifically, the data correspond to the anticipated results presented in the paper submitted to Nature Protocols, The present dataset corresponds to the anticipated results that can be obtained following the protocol described in the article. In particular, dataset corresponding to Figure 8 presents the absorbance values from the WST-1 cytotoxicity assay as a function of the well size where the experiment is performed. Data from Figure 9b show absorbance values from the WST-1 cytotoxicity assay as a function of the treatment time. Data from figure 9c-e show the absorbance or fluorescence values obtained in the experiments to quantify RONS, together with the values from the calibration curves. Data fromfigure 10 a provide the list and values of mRNA fold induction of the genes analyzed, and data from figure 10c show the pixel intensity obtained for the phospho-kinase antibody array., 1

These data are associated to the paper with the same title of the authors, and are structured according to the figures in the paper: http://hdl.handle.net/2117/335727, The use of Cold Atmospheric Plasma (CAP) in oncology is being recently studied as a novel potential anti-cancer therapy. However, the beneficial effects of CAP for treating osteosarcoma have mostly been demonstrated in 2-dimensional cultures of cells, which do not mimic the complexity of the 3-dimensional (3D) bone microenvironment. In order to evaluate the effects of CAP in a relevant context of the human disease, we developed a 3D tissue-engineered model of osteosarcoma using a bone-like scaffold made of collagen type I and hydroxyapatite nanoparticles. Human osteosarcoma cells cultured within the scaffold showed a high capacity to infiltrate and proliferate and to exhibit osteomimicry in vitro. As expected, we observed significantly different functional behaviors between monolayer and 3D cultures when treated with Cold Plasma-Activated Ringer’s Solution (PAR). Our data reveal that the 3D environment not only protects cells from PAR-induced lethality by scavenging and diminishing the amount of reactive oxygen and nitrogen species generated by CAP, but also favours the stemness phenotype of osteosarcoma cells. This is the first study that demonstrates the negative effect of PAR on cancer stem-like cell subpopulations in a 3D biomimetic model of cancer. These findings will allow to suitably re-focus research on plasma-based therapies in future, 1