Methionine supplementation during the suckling period of ewe lambs might program feed efficiency during the whole life time due to stable changes of epigenetic marks caused by methylation of DNA. The results of the present study revealed that methionine supplementation during the suckling period has a limited effect on epigenetic marks of DNA obtained from blood., This work was funded by Ministerio de Ciencia e Innovación (PID2021-126489OB-I00, MCIN/AEI/10.13039/501100011033, “FEDER, Una manera de hacer Europa”); ACRONYM: NUPROVI
Alba Martín gratefully acknowledges receipt of a pre-doctoral grant (PRE2019-089288) from Ministerio de Ciencia e Innovación (MCIN/AEI/10.13039/501100011033, “El FSE invierte en tu futuro”); CSIC is acknowledged for supporting Open Access publication., 3_****_L001_R1_001.fastq 3_****_L001_R2_001.fastq 4_****_L001_R1_001.fastq 4_****_L001_R2_001.fastq 16_****_L001_R1_001.fastq 16_****_L001_R2_001.fastq 17_****_L001_R1_001.fastq 17_****_L001_R2_001.fastq 20_****_L001_R1_001.fastq 20_****_L001_R2_001.fastq 21_****_L001_R1_001.fastq 21_****_L001_R2_001.fastq 29_****_L001_R1_001.fastq 29_****_L001_R2_001.fastq 30_****_L001_R1_001.fastq 30_****_L001_R2_001.fastq 31_****_L001_R1_001.fastq 31_****_L001_R2_001.fastq 32_****_L001_R1_001.fastq 32_****_L001_R2_001.fastq, No

Methionine supplementation during the suckling period of ewe lambs (F0) may modify DNA methylation in the germline (F0) during this critical window period for the neonate, hence promoting inter-generational transmission of epigenetic marks to the offspring (F1). This factor may alter the expression of specific genes and physiological traits throughout life of the offspring (F1). The results of the present study revealed that methionine supplementation during the suckling period of ewe lambs (F0) caused the transmission of epigenetic marks to the offspring (DNA obtained from the blood of 1-month old Assaf ewe lambs, F1), so genes involved in Developmental process and Anatomical structure development were differentially methylated in F1-MET when compared to F1-CTRL ewe lambs. Therefore, although the effects on feed efficiency during adulthood were limited, F1-MET dairy sheep showed a reduced live body weight during the whole life., This work was funded by Ministerio de Ciencia e Innovación (PID2021-126489OB-I00, MCIN/AEI/10.13039/501100011033, “FEDER, Una manera de hacer Europa”); ACRONYM: NUPROVI
Alba Martín gratefully acknowledges receipt of a pre-doctoral grant (PRE2019-089288) from Ministerio de Ciencia e Innovación (MCIN/AEI/10.13039/501100011033, “El FSE invierte en tu futuro”); CSIC is acknowledged for supporting Open Access publication., Relationship between files, if important: S_1018_1.fastq (control group, F1-CTRL) S_1018_2.fastq (control group, F1-CTRL) S_1020_1.fastq (control group, F1-CTRL) S_1020_2.fastq (control group, F1-CTRL) S_1022_1.fastq (control group, F1-CTRL) S_1022_2.fastq (control group, F1-CTRL) S_1025_1.fastq (control group, F1-CTRL) S_1025_2.fastq (control group, F1-CTRL) S_1026_1.fastq (control group, F1-CTRL) S_1026_2.fastq (control group, F1-CTRL) S_1033_1.fastq (methionine group, F1-MET) S_1033_2.fastq (methionine group, F1-MET) S_1038_1.fastq (methionine group, F1-MET) S_1038_2.fastq (methionine group, F1-MET) S_1042_1.fastq (methionine group, F1-MET) S_1042_2.fastq (methionine group, F1-MET) S_1043_1.fastq (methionine group, F1-MET) S_1043_2.fastq (methionine group, F1-MET) S_1045_1.fastq (methionine group, F1-MET) S_1045_2.fastq (methionine group, F1-MET), Peer reviewed

Methionine supplementation during the suckling period of ewe lambs (F0) might program persistent changes on DNA methylation, thus modifying miRNA profile of colostrum exosomes during the lactation period when these animals are adults. Identifying these changes is important because may drive some effects on the offspring (F1) under natural rearing conditions. The results of the present study revealed changes in miRNA profile of colostrum exosomes as a consequence of methionine supplementation during the suckling period of ewe lambs (F0)., This work was funded by Ministerio de Ciencia e Innovación (PID2021-126489OB-I00, MCIN/AEI/10.13039/501100011033, “FEDER, Una manera de hacer Europa”); ACRONYM: NUPROVI
Alba Martín gratefully acknowledges receipt of a pre-doctoral grant (PRE2019-089288) from Ministerio de Ciencia e Innovación (MCIN/AEI/10.13039/501100011033, “El FSE invierte en tu futuro”); CSIC is acknowledged for supporting Open Access publication., 1M_L001_R1_001.fastq.gz (F0-MET) 1M_L001_R2_001.fastq.gz (F0-MET) 8C_L001_R1_001.fastq.gz (F0-CTRL) 8C_L001_R2_001.fastq.gz (F0-CTRL) 19C_L001_R1_001.fastq.gz (F0-CTRL) 19C_L001_R2_001.fastq.gz (F0-CTRL) 28M_L001_R1_001.fastq.gz (F0-MET) 28M_L001_R2_001.fastq.gz (F0-MET) 31M_L001_R1_001.fastq.gz (F0-MET) 31M_L001_R2_001.fastq.gz (F0-MET) 35C_L001_R1_001.fastq.gz (F0-CTRL) 35C_L001_R2_001.fastq.gz (F0-CTRL), Peer reviewed

This work was funded by Ministerio de Ciencia e Innovación (PID2021-126489OB-I00, MCIN/AEI/10.13039/501100011033, “FEDER, Una manera de hacer Europa”); ACRONYM: NUPROVI
Alba Martín gratefully acknowledges receipt of a pre-doctoral grant (PRE2019-089288) from Ministerio de Ciencia e Innovación (MCIN/AEI/10.13039/501100011033, “El FSE invierte en tu futuro”); CSIC is acknowledged for supporting Open Access publication., File list:H1_1001_R1.fastq; H1_1001_R2.fastq; H2_1001_R1.fastq; H2_1001_R2.fastq; H3_1001_R1.fastq; H3_1001_R2.fastq; H4_1001_R1.fastq; H4_1001_R2.fastq; H5_1001_R1.fastq; H5_1001_R2.fastq; ORG1_1001_R1.fastq; ORG1_1001_R2.fastq; ORG2_1001_R1.fastq; ORG2_1001_R2.fastq; ORG3_1001_R1.fastq; ORG3_1001_R2.fastq; ORG4_1001_R1.fastq; ORG4_1001_R2.fastq; ORG5_1001_R1.fastq; ORG5_1001_R2.fastq;, Peer reviewed

Twenty-eight pregnant Merino ewes were used to study the effect of including 4 g/kg of betaine in a complete pelleted ration on feed intake and animal performance of both, dams (F0) and female offspring (F1), including the first lactation period. To this end, fourteen ewes (F0-CTL group) received a complete pelleted ration (control diet) ad libitum during the last two months of gestation. The remaining animals (F0-BET group; n = 14) received the same diet, but with the addition of 4 g/kg of betaine. The results of the present study revealed that betaine supplementation during the gestation period of Merino ewes caused differentially methylated regions (DMRs) in the female offspring that were assessed at 53,649 and 2164 sites using MethylKit (q value < 0.05) and CGmap Tools (FDR < 0.05), respectively., This work was funded by Ministerio de Ciencia e Innovación (PID2021-126489OB-I00, MCIN/AEI/10.13039/501100011033, “FEDER, Una manera de hacer Europa”); ACRONYM: NUPROVI
CSIC is acknowledged for supporting Open Access publication., File list: S_1006_1.fastq; S_1006_2.fastq; S_1010_1.fastq; S_1010_2.fastq; S_1015_1.fastq; S_1015_2.fastq; S_1020_1.fastq; S_1020_2.fastq; S_1023_1.fastq; S_1023_2.fastq; S_1055_1.fastq; S_1055_2.fastq; S_1064_1.fastq; S_1064_2.fastq; S_1065_1.fastq; S_1065_2.fastq; S_1066_1.fastq; S_1066_2.fastq; S_1074_1.fastq; S_1074_2.fastq;, Peer reviewed