BES-2015-074411
BES-2015-074411
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Nombre agencia financiadora Ministerio de Economía y Competitividad
Acrónimo agencia financiadora MINECO
Programa Programa Estatal de Promoción del Talento y su Empleabilidad
Subprograma Subprograma Estatal de Formación
Convocatoria Contratos predoctorales para la formación de doctores (2015)
Año convocatoria 2015
Unidad de gestión Dirección General de Investigación Científica y Técnica
Centro beneficiario UNIVERSIDAD PÚBLICA DE NAVARRA (UPNA)
Centro realización DEPARTAMENTO CIENCIAS DEL MEDIO NATURAL
Identificador persistente http://dx.doi.org/10.13039/501100003329
Publicaciones
Resultados totales (Incluyendo duplicados): 1
Encontrada(s) 1 página(s)
Encontrada(s) 1 página(s)
Increased ascorbate biosynthesis does not improve nitrogen fixation nor alleviate the effect of drought stress in nodulated Medicago truncatula plants
Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
- Cobos Porras, Inmaculada Libertad
- Rubia Galiano, María Isabel
- Huertas, Raúl
- Kum, David
- Dalton, David A.
- Udvardi, Michael
- Arrese-Igor Sánchez, César
- Larrainzar Rodríguez, Estíbaliz
Legume plants are able to establish nitrogen-fixing symbiotic relations with Rhizobium
bacteria. This symbiosis is, however, affected by a number of abiotic constraints,
particularly drought. One of the consequences of drought stress is the overproduction
of reactive oxygen (ROS) and nitrogen species (RNS), leading to cellular damage and,
ultimately, cell death. Ascorbic acid (AsA), also known as vitamin C, is one of the
antioxidant compounds that plants synthesize to counteract this oxidative damage. One
promising strategy for the improvement of plant growth and symbiotic performance
under drought stress is the overproduction of AsA via the overexpression of enzymes
in the Smirnoff-Wheeler biosynthesis pathway. In the current work, we generated
Medicago truncatula plants with increased AsA biosynthesis by overexpressing MtVTC2,
a gene coding for GDP-L-galactose phosphorylase. We characterized the growth and
physiological responses of symbiotic plants both under well-watered conditions and
during a progressive water deficit. Results show that increased AsA availability did not
provide an advantage in terms of plant growth or symbiotic performance either under
well-watered conditions or in response to drought., This work had been funded by the Spanish Ministry of Science and Innovation-European Regional Development Fund (grant RTI2018-094623-B-C22) and the Government of Navarra (project PC112-113 LEGUSI). EL was a Ramón y Cajal fellow (RYC2018-023867-I) and LC-P and MR were Formación de Personal Investigador fellows from the Spanish Ministry of Economy and Competitiveness (BES-2015-074411 and BES-2012-059972, respectively).
bacteria. This symbiosis is, however, affected by a number of abiotic constraints,
particularly drought. One of the consequences of drought stress is the overproduction
of reactive oxygen (ROS) and nitrogen species (RNS), leading to cellular damage and,
ultimately, cell death. Ascorbic acid (AsA), also known as vitamin C, is one of the
antioxidant compounds that plants synthesize to counteract this oxidative damage. One
promising strategy for the improvement of plant growth and symbiotic performance
under drought stress is the overproduction of AsA via the overexpression of enzymes
in the Smirnoff-Wheeler biosynthesis pathway. In the current work, we generated
Medicago truncatula plants with increased AsA biosynthesis by overexpressing MtVTC2,
a gene coding for GDP-L-galactose phosphorylase. We characterized the growth and
physiological responses of symbiotic plants both under well-watered conditions and
during a progressive water deficit. Results show that increased AsA availability did not
provide an advantage in terms of plant growth or symbiotic performance either under
well-watered conditions or in response to drought., This work had been funded by the Spanish Ministry of Science and Innovation-European Regional Development Fund (grant RTI2018-094623-B-C22) and the Government of Navarra (project PC112-113 LEGUSI). EL was a Ramón y Cajal fellow (RYC2018-023867-I) and LC-P and MR were Formación de Personal Investigador fellows from the Spanish Ministry of Economy and Competitiveness (BES-2015-074411 and BES-2012-059972, respectively).