CARACTERIZACION MOLECULAR DE LA FUNCION DEL SISTEMA SENSORIAL DE DOS-COMPONENTES EN EL COMPORTAMIENTO MULTICELULAR Y LA VIRULENCIA DE STAPHYLOCOCCUS AUREUS

BIO2008-05284-C02-01

Nombre agencia financiadora Ministerio de Ciencia e Innovación
Acrónimo agencia financiadora MICINN
Programa Programa Nacional de Investigación Fundamental
Subprograma Investigación fundamental no-orientada
Convocatoria Investigación fundamental no-orientada
Año convocatoria 2008
Unidad de gestión Subdirección General de Proyectos de Investigación
Centro beneficiario UNIVERSIDAD PÚBLICA DE NAVARRA (UPNA)
Centro realización INSTITUTO DE AGROBIOTECNOLOGÍA Y RECURSOS NATURALES (IARN)
Identificador persistente http://dx.doi.org/10.13039/501100004837

Publicaciones

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Wavelet-based detection of transcriptional activity on a novel Staphylococcus aureus tiling microarray

Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
  • Segura, Víctor
  • 0000-0001-8148-6281
  • Uzqueda, Maite
  • 0000-0002-6625-9221
  • Muñoz Barrutia, Arrate
UPNa. Instituto de Agrobiotecnología. Laboratorio de Biofilms Microbianos, Incluye 7 ficheros de datos, Background: High-density oligonucleotide microarray is an appropriate technology for genomic analysis, and is particulary useful in the generation of transcriptional maps, ChIP-on-chip studies and re-sequencing of the genome. Transcriptome analysis of tiling microarray data facilitates the discovery of novel transcripts and the assessment of differential expression in diverse experimental conditions. Although new technologies such as next-generation sequencing have appeared, microarrays might still be useful for the study of small genomes or for the analysis of genomic regions with custom microarrays due to their lower price and good accuracy in expression quantification. Results: Here, we propose a novel wavelet-based method, named ZCL (zero-crossing lines), for the combined denoising and segmentation of tiling signals. The denoising is performed with the classical SUREshrink method and the detection of transcriptionally active regions is based on the computation of the Continuous Wavelet Transform (CWT). In particular, the detection of the transitions is implemented as the thresholding of the zero-crossing lines. The algorithm described has been applied to the public Saccharomyces cerevisiae dataset and it has been compared with two well-known algorithms: pseudo-median sliding window (PMSW) and the structural change model (SCM). As a proof-of-principle, we applied the ZCL algorithm to the analysis of the custom tiling microarray hybridization results of a S. aureus mutant deficient in the sigma B transcription factor. The challenge was to identify those transcripts whose expression decreases in the absence of sigma B. Conclusions: The proposed method archives the best performance in terms of positive predictive value (PPV) while its sensitivity is similar to the other algorithms used for the comparison. The computation time needed to process the transcriptional signals is low as compared with model-based methods and in the same range to those based on the use of filters. Automatic parameter selection has been incorporated and moreover, it can be easily adapted to a parallel implementation. We can conclude that the proposed method is well suited for the analysis of tiling signals, in which transcriptional activity is often hidden in the noise. Finally, the quantification and differential expression analysis of S. aureus dataset have demonstrated the valuable utility of this novel device to the biological analysis of the S. aureus transcriptome., This work was supported by the Spanish Torres-Quevedo fellowship [PTQ-08-03-07769] to VS. ATA and AMB were supported by Spanish Ministry of Science and Innovation ‘Ramón y Cajal’ contracts. This work was supported by the Spanish Ministry of Science and Innovation Grants BIO2008-05284-C02-01, BFU2011-23222, ERA-NET Pathogenomics PIM2010EPA-00606 and the agreement between ‘Fundación para la Investigación médica aplicada’ (FIMA) and the ’UTE project CIMA’.