BUSCADOR RECOLECTA

The development of three-dimensional environments to mimic the in vivo cellular response is a problem in the building of disease models. This study aimed to synthesize and validate three-dimensional support for culturing monoclonal plasma cells (mPCs) as a disease model for multiple myeloma. The three-dimensional environment is a biomimetic microgel formed by alginate microspheres and produced on a microfluidic device whose surface has been functionalized by a layer-by-layer process with components of the bone marrow’s extracellular matrix, which will interact with mPC. As a proof of concept, RPMI 8226 cell line cells were cultured in our 3D culture platform. We proved that hyaluronic acid significantly increased cell proliferation and corroborated its role in inducing resistance to dexamethasone. Despite collagen type I having no effect on proliferation, it generated significant resistance to dexamethasone. Additionally, it was evidenced that both biomolecules were unable to induce resistance to bortezomib. These results validate the functionalized microgels as a 3D culture system that emulates the interaction between tumoral cells and the bone marrow extracellular matrix. This 3D environment could be a valuable culture system to test antitumoral drugs efficiency in multiple myeloma.

Dataset for Case study A (UV data)

This folder contains an annotated corpora composed by six with six different debates in their orginal languages about a variety of cultural objects related with the following historical figures. More information about this research can be found in the following papers: (i) Budzynska, K., Koszowy, M. & Pereira-Fariña, M. Associating Ethos with Objects: Reasoning from Character of Public Figures to Actions in the World. Argumentation (2021). https://doi.org/10.1007/s10503-021-09552-4; (ii) Pereira-Fariña, M. Koszowy, M. & Budzynska, K. ‘It was Never Just About the Statue’ Ethos of Historical Figures in Public Debates on Contested Cultural Objects. Discourse & Society. Fortcoming.

Retrospective data from 147 selected patients that underwent strabismus surgery between 2013-2016. Includes information concerning the type and onset of the strabismus, relevant medical records, awake state eye deviation and the intraoperative strabismus inspection.