BUSCADOR RECOLECTA

Dataset of bone stable isotope values (δ13C and δ15N) and palate breadth for twelve marine mammal species (Otaria flavescens, Arctocephalus australis, Pontoporia blainvillei, Phocoena spinipinnis, Delphinus delphis, Lagenodelphis hosei, Pseudorca crassidens, Tursiops truncatus, Monachus monachus, Phocoena phocoena, Stenella frontalis and Sousa teuszii) from Uruguay and Mauritania. δ13Ccor: isotope values corrected for Suess effect shifts.

Palate breadth of South American sea lions (Otaria flavescens), South American fur seals (Arctocephalus australis) and Franciscana dolphins (Pontoporia blainvillei) from Río de la Plata estuary. Stable isotope values (δ13C and δ15N) and standard length of the Franciscana dolphin (Pontoporia blainvillei) and potential prey species off Río de la Plata estuary. δ13C and δ15N: values not corrected for isotopic baseline shifts; δ13Ccor and δ15Ncor: values corrected for isotopic baseline shifts.

The bone stable oxygen isotope values and the distance of the stranding site from the innermost point of the estuary for thirteen marine mammal species (Otaria flavescens, Arctocephalus australis, Pontoporia blainvillei, Ziphius cavirostris, Phocoena dioptrica, Phocoena spinipinnis, Delphinus delphis, Globicephala melas, Grampus griseus, Lagenodelphis hosei, Orcinus orca, Pseudorca crassidens and Tursiops truncatus) from the Río de la Plata estuary and the adjoining western South Atlantic Ocean. Stable oxygen isotope values (δ18O) are reported relative to the Vienna Standard Mean Oceanic Water (V-SMOW).

Tabla de datos, Peer reviewed

1 table., S2 Table. NAAIRS mutants of SAUR63 and root tortuosity index., Peer reviewed

1 table., S3 Table. Primers used., Peer reviewed

1 table., S4 Table. Clones for transformation., Peer reviewed

1 table., S5 Table. Antibodies., Peer reviewed

A) Genomic map showing positions of genes and locations of mutations from CRISPR/Cas9 mutagenesis in the 9x-saur mutant based on the TAIR10 Arabidopsis genome annotation. The first sgRNA directed cuts in both SAUR61 and SAUR64 (green arrows), creating a deletion between them (green bar) and leaving behind a hybrid gene with a frameshift at the junction site (symbolized by a green X). The second sgRNA directed cuts in the remaining genes (blue arrows, with lighter blue indicating slight mismatches between the sgRNA and the genome), leading to deletions (blue bars) and/or frameshift mutations (blue X’s). SAUR gene names are abbreviated as S61 etc. SAUR61-SAUR68 are on chromosome 1 and SAUR75 is on chromosome 5. B,C) 5-day-old seedlings grown on 1x MS/1% Suc medium in long days. Scale bar, 1 mm. D) Hypocotyl lengths of seedlings grown for 4d in short days on 0.5x MS medium. n, 27 (wild type), 22 (9x-saur). E) Cotyledon area of seedlings grown on vertically oriented plates for 6d on MS/1% Suc medium. n, 16 (wild type), 22 (9x-saur). Graphs show means ± s.d. No statistical differences were detected between wild-type and 9x-saur mutant measurements by t-test. F) Sequences of guide RNAs used for CRISPR/Cas9-mediated mutagenesis, wild-type genes, and mutant alleles present in the 9x-saur mutant. Underlines indicate PAM motif adjacent to guide RNA target site, and any mismatches to the guide RNA sequence. Uppercase bold letters indicate insertion mutations. All alleles create frameshift mutations except for saur75-1, which has an in-frame deletion of 13 amino acids in the SAUR domain., Peer reviewed

A-F) Each point represents the length (A, C, E) or width (B, D, F) of a single epidermal cell against distance of that cell from the base of the hypocotyl (x-axis). Seedlings were grown in short days for 2 (A, B), 3 (C, D) or 4 (E, F) days. Cells were measured from confocal z-stack projections using ML1:RFP PM-localized fluorescence (examples in S3G, S3H and S3I Fig). Data are from 3 cell files per seedling, for two seedlings per time point, except that wild type at 4 days and ost2-2 at 3 days data are from just one seedling each. In a replicate experiment with 2-day-old seedlings only, P35S:SAUR63:YFP:HA hypocotyls also had a greater proportion of longer cells than did wild-type hypocotyls., Peer reviewed