BUSCADOR RECOLECTA

Additional file 1: Supplementary Fig. 1. Study design and Flow-chart., Consejería de Transformación Económica, Industria, Conocimiento y Universidades Instituto de Salud Carlos III Consejería de Salud y Familias, Junta de Andalucía, Peer reviewed

Additional file 2: Supplementary Table 1. Demographical and anthropometrics characteristics of the study population., Consejería de Transformación Económica, Industria, Conocimiento y Universidades Instituto de Salud Carlos III Consejería de Salud y Familias, Junta de Andalucía, Peer reviewed

Additional file 3: Supplementary Table 2. Comorbidities and behavioral factors of residents from the Santa Caridad Nursing Home., Consejería de Transformación Económica, Industria, Conocimiento y Universidades Instituto de Salud Carlos III Consejería de Salud y Familias, Junta de Andalucía, Peer reviewed

Additional file 4: Supplementary Table 3.Thymic activity, Biochemical, Inflammatory and Immunological profiles of the study populations one month after the second dose (T1)., Consejería de Transformación Económica, Industria, Conocimiento y Universidades Instituto de Salud Carlos III Consejería de Salud y Familias, Junta de Andalucía, Peer reviewed

Additional file 5: Supplementary Fig. 2. Levels of Thymosin-α1 in higher vs. lower responders of the older group., Consejería de Transformación Económica, Industria, Conocimiento y Universidades Instituto de Salud Carlos III Consejería de Salud y Familias, Junta de Andalucía, Peer reviewed

Additional file 6: Supplementary Fig. 3. Correlations among Log of anti-S antibody titers at all different study points., Consejería de Transformación Económica, Industria, Conocimiento y Universidades Instituto de Salud Carlos III Consejería de Salud y Familias, Junta de Andalucía, Peer reviewed

Additional file 7: Supplementary Fig. 4. Factors associated with the magnitude of the initial response to the BNT162B2 vaccine at T1 by age-groups., Consejería de Transformación Económica, Industria, Conocimiento y Universidades Instituto de Salud Carlos III Consejería de Salud y Familias, Junta de Andalucía, Peer reviewed

Additional file 8. Detailed methodology., Consejería de Transformación Económica, Industria, Conocimiento y Universidades Instituto de Salud Carlos III Consejería de Salud y Familias, Junta de Andalucía, Peer reviewed

(A) Scheme representing the fluorescence microscopy experiments comparing the distribution of 3Dpol in the presence of 3AB1, wild type and mutant. The left panel shows 3Dpol interacting with the 3AB1 precursor bound to the membrane. The right panel mimics the scenario in the presence of the 3AB1 mutant 3AB1(P6S/R9A/R16A/L19S), unable to bind 3Dpol. (B) Fluorescence images of HeLa cells showing the different distribution of 3Dpol bound to 3AB1 wild type or the 3AB1(P6A/R9A/R16A/L19S) mutant. Upper panels show the control cells transfected with the polymerase only (green), which appears distributed throughout the cell. Middle panels show cells transfected with 3AB1 wild type (red) and 3Dpol (green), where 3Dpol mostly co-localizes with the 3AB1 protein in a continuous compartment in the cytoplasm. The lower panels show cells transfected with the 3Dpol and the 3AB1 mutant (P6A/R9A/R16A/L19S), where 3Dpol recovers its localization throughout the cell. The images shown are representative of the total number of images obtained. (C) Fluorescence Intensity plots comparing the relative distribution of 3Dpol (green) in presence of wild type and mutant 3AB1 proteins (red), left and right panels, respectively. The nucleus is labelled in blue (DAPI)., Peer reviewed

E.coli membranes containing, wild-type 3AB1 or 3AB1(P6S/R9A), 3AB1(R16A/L19S) or 3AB1(P6S/R9A/R16A/L19S) mutants, disrupting the 3B1-3Dpol binding site were used in the analysis. (A) Western blot experiment that shows the 3Dpol protein recruited to the membrane. (B) Bars diagram indicating the percentage of 3Dpol bound by the membranes. To calculate the fraction of 3Dpol bound to 3AB, the volume of the 3Dpol band from a control membrane was subtracted from each of the other 3Dpol bands, and these were then normalized to the 3Dpol band pulled down by wild-type 3AB. Assays were performed in triplicate, and the mean value and the standard deviation are reported., Peer reviewed