BUSCADOR RECOLECTA

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Open access. Please, contact corresponding author in case of doubts, Description: The Malaspina2010_chlorophyll.xlsx file contains the fluorimetric chlorophyll a data of the circunnavigation expedition Malaspina 2010, which took place between 14/12/2010 and 14/07/2011 on board the BIO Hespérides. The data presented here were obtained between 16/12/2010 and 11/07/2011. The date and position of the sampling stations are listed in the third sheet of the file. Methods: Water samples for fluorimetric chlorophyll a (Chl a) determination were collected from 3 m depth with a 30-liter Niskin bottle and from selected depths between 10 and 200 m with a Rosette of 24 10-liter Niskin bottles attached to a CTD probe. Chl a determination was carried out as described in Estrada et al. (2012). Briefly, between 200 and 500 cm3 of seawater were filtered through GF/F glass fiber filters that were subsequently frozen at -20°C and, after a minimum of 6 hours, introduced in vials with acetone 90% and left for 24 hours in the dark, at 4ºC. The Chl a concentration in the acetonic extracts was measured with a Turner Designs fluorimeter calibrated with a pure Chl a standard (Sigma-Aldrich); no phaeopigment correction was applied. Size-fractionated analyses of Chl a were performed for samples from surface, the 20% surface PAR and the deep chlorophyll maximum. The analyses were carried out by sequential filtration of a 500 cm3 of seawater through Poretics (polycarbonate) membrane filters of pore sizes 20 μm, 2 μm and 0.2 μm, which were subsequently treated as the GF/F ones (Estrada, 2012), This work was supported by Consolider-Ingenio 2010, CSD2008-00077 of the former Spanish Ministerio de Ciencia e Innovación (ES) (now www.ciencia.gob.es) and the Consejo Superior de Investigaciones Científicas (CSIC) (ES), Authors; Methods; Station positions; leg 1; leg 2; Legs 3-4; leg 5; leg 6; leg 7; References, Peer reviewed