BUSCADOR RECOLECTA

A. Proportion of samples for which each of the known miRNAs across the different groups were detected. B. Cumulative abundance of the known feline miRNAs. The dots indicate the log10 of the miRNA abundance for each miRNA. miRNAs are sorted in each group in a decreasing order by their miRNA abundance on the x-axis, independently for each group. C. Proportion of samples for which each of the putative novel miRNA candidates across the different groups were detected. D. Cumulative abundance of the putative novel miRNAs. The dots indicate the log10 of the miRNA abundance for each miRNA. miRNAs are sorted in each group in a decreasing order by their miRNA abundance on the x-axis, independently for each group. CKD: Chronic kidney disease; PN: Pyelonephritis; SB/C: Subclinical bacteriuria/Cystitis; UO: Ureteral obstruction, CPM: Counts per million., Peer reviewed

1 figure., All samples together (all groups), as well as each one of the contrasts considered (Controls vs. PN; Control vs. SB/C; Control vs. UO; Control vs. CKD; PN vs. SB/C; PN vs. UO; PN vs. CKD and PN vs. other Pathologies) are shown. CKD: Chronic kidney disease; PN: Pyelonephritis; SB/C: Subclinical bacteriuria/Cystitis; UO: Ureteral obstruction., Peer reviewed

1 figure., CKD: Chronic kidney disease; PN: Pyelonephritis; SB/C: Subclinical bacteriuria/Cystitis; UO: Ureteral obstruction, CPM: Counts per million, Rq: Relative quantities., Peer reviewed

The data presented is from selected miRNAs that were DA (|log2FC| ≥ 1.5 for qPCR and |log2FC| ≥ 2 for small RNAseq; q-value < 0.05) using both methodologies. CKD: Chronic kidney disease; PN: Pyelonephritis; SB/C: Subclinical bacteriuria/Cystitis; UO: Ureteral obstruction, CPM: Counts per million, Rq: Relative quantities., Peer reviewed

1 table., The table includes for each miRNA the arguments for its selection for further validation, the forward and reverse sequence, the miRBase sequence used as template for primer design, if successful miRNA amplification was obtained with qPCR and qPCR amplification efficiency., Peer reviewed

1 table, Total RNA was isolated from whole urine of 38 cats, and 6 μl of each RNA sample was applied in small RNAseq. Concentration and ratios were obtained using Nanodrop measurement., Peer reviewed

1 table., Reads mapped to the different gene biotypes in the genome are included. SD: standard deviation; rRNA: ribosomal RNA; tRNA: transfer RNA; snoRNA: small nucleolar RNA; snRNA: small nuclear RNA; RE: repeat elements; miRNA: microRNA., Peer reviewed

1 table., mirDeep2 output of the 996 miRNAs identified with information from their sequences, scores and probabilities of being a true positive miRNA, among others., Peer reviewed

1 table., Including the miRNA ID name based on sequence homology with other species (blastn), miRNA coordinates, strand, closest homology miRNA with their percentage of identity and probability (E-value) and consensus mature, star and precursor sequences., Peer reviewed

1 table., This includes information from their source (database/study/de novo) and their genomic coordinates. CPM: Counts Per Million., Peer reviewed