BUSCADOR RECOLECTA

Peer reviewed

File List: Fig 1 F1b: Size histogram of Ba40Dy60 NPs F1c: EDX spectrum of Ba40Dy60 NPs F1e: Size histogram of Ba51Dy49 NPs F1f: F1c: EDX spectrum of Ba51Dy49 NPs Fig 2 F2: Experimental XRD patterns of Ba40Dy60 and Ba51Dy49 samples Fig 3 F3b: XRD patterns of the precipitates obtained in the conditions used for the synthesis of Ba51Dy49 NPs but using different aging times F3c: Unit cell parameters and Ba/(Ba+Dy) ratio of the precipitates versus aging time Fig 4 F4a: DLS intensity curves of the Ba51Dy49 NPs suspended in water after different periods of time at rest F4b: FTIR spectrum of the Ba51Dy49 NPs Fig 5 F5g: Total number of cells per well after exposure to increasing concentration of NPs F5h: Percentage of dead cells after exposure to increasing concentration of Ba51Dy49 NPs. F5i: MTT assay of cells exposed to increasing concentration of Ba51Dy49 NPs Fig 6 F6a: 1/T2 values obtained in aqueous suspensions of Ba51Dy49 NPs at 9.4 T and 1.44 T versus Dy concentration of the suspensions Fig 7 F7b: X-ray attenuation at 80 kVp, in Hounsfield Units (HU), of aqueous suspensions containing Ba51Dy49 NPs and Iohexol, versus suspension concentration. F7c: X-ray attenuation, in HU, of aqueous suspensions containing 20.0 mg·mL-1 of Ba51Dy49 and DyF3 NPs, versus working voltage, Bimodal medical imaging based on Magnetic Resonance Imaging (MRI) and Computed Tomography (CT) is a well-known strategy to increase diagnostic accuracy. The most recent advances in MRI and CT instrumentation are related to the use of ultrahigh magnetic fields (UHF-MRI) and different working voltages (spectral CT), respectively. Such advances require the parallel development of bimodal contrast agents (CAs) that are efficient under the new instrumental conditions. In this work, we have synthesized, through a precipitation reaction from a glycerol solution of the precursors, uniform barium dysprosium fluoride nanospheres with cubic fluorite structure, whose size was found to depend on the Ba/(Ba+Dy) ratio of the starting solution. Moreover, irrespective of the starting Ba/(Ba+Dy) ratio, the experimental Ba/(Ba+Dy) values were always lower than those used in the starting solutions. This result was assigned to a lower precipitation kinetics of barium fluoride compared to dysprosium fluoride, as inferred from the detailed analysis of the effect of reaction time on the chemical composition of the precipitates. A sample composed of 34 nm nanospheres with a Ba0.51Dy0.49F2.49 stoichiometry, showed a transversal relaxivity (r2) value of 147.11 mM-1·s-1 at 9.4 T and gave a high negative contrast in the phantom image. Likewise, it produced high X-ray attenuation in a large range of working voltages (from 80 KVp to 140 KVp), which can be attributed to the presence of different K-edge values, high Z elements (Ba and Dy) in the nanospheres. Finally, these nanospheres showed negligible cytotoxicity for different biocompatibility tests. Taken together, these results show that the reported nanoparticles (NPs) are excellent candidates as UHF MRI/Spectral CT bimodal imaging CAs., This publication is part of the I + D + I Grants PID2021-122328OB-I00 and PID2020-118448RB-C21 funded by MCIN/AEI/10.13039/501100011033 and by “ERDF A way of making Europe”. This work was supported as well by Junta de Andalucía under grant no. P20_00182, co-financed by EU FEDER funds. High-field relaxivity measurements were performed at the ICTS “NANBIOSIS”, specifically in Unit 28 at the “Instituto de Investigación Biomédica de Málaga y Plataforma en Nanomedicina (IBIMA Plataforma BIONAND)”., Peer reviewed

The N-terminal tails of eukaryotic histones are frequently posttranslationally modified. The role of these modifications in transcriptional regulation is well-documented. However, the extent to which the enzymatic processes of histone posttranslational modification might affect metabolic regulation is less clear. Here, we investigated how histone methylation might affect metabolism using metabolomics, proteomics, and RNA-seq data from cancer cell lines, primary tumour samples and healthy tissue samples. In cancer, the expression of histone methyltransferases (HMTs) was inversely correlated to the activity of NNMT, an enzyme previously characterised as a methyl sink that disposes of excess methyl groups carried by the universal methyl donor S-adenosyl methionine (SAM or AdoMet). In healthy tissues, histone methylation was inversely correlated to the levels of an alternative methyl sink, PEMT. These associations affected the levels of multiple histone marks on chromatin genome-wide but had no detectable impact on transcriptional regulation. We show that HMTs with a variety of different associations to transcription are co-regulated by the Retinoblastoma (Rb) tumour suppressor in human cells. Rb-mutant cancers show increased total HMT activity and down-regulation of NNMT. Together, our results suggest that the total activity of HMTs affects SAM metabolism, independent of transcriptional regulation., Peer reviewed

The excel file includes different spreadsheet including, first, every stalagmite (age model, d18O and d13C, Mg/Ca and other trace elements when available) and, second, every composite record produced by ISCAM. The word document is the U-Th table with all the dates used in constructing the age models. This dataset corresponds to our manuscript published as a preprint in Climate of the Past: https://cp.copernicus.org/preprints/cp-2023-54/, Peer reviewed

1 file, The Cape Verde Frontal Zone (CVFZ) is a highly dynamic region located in the southern boundary of the Canary Current Eastern Boundary Upwelling Ecosystem. Due to the interaction of the Cape Verde Front with the Mauritanian coastal upwelling, the area features large vertical and horizontal export fluxes of organic matter. Full-depth profiles were recorded during FLUXES I cruise, with four consecutive transects defining a box embracing the giant filament of Cape Blanc and the Cape Verde front. Fifteen levels were sampled in medium and long stations (down to 4000 dbar) and 10 levels in short stations (down to 2000 dbar) where inorganic nutrients (NO3, NO2, Si(OH)4 and PO4), dissolved organic carbon/total dissolved nitrogen (DOC/TDN), and suspended particulate organic carbon and nitrogen (POC and PON) were sampled. Micromolar concentrations of nutrient salts were determined simultaneously by segmented flow analysis in an Alliance Futura autoanalyser. The determination of suspended POC and PON was carried out by high temperature catalytic oxidation at 900 °C in a Perkin Elmer 2400 elemental analyser. DOC and TDN were analysed by high temperature catalytic oxidation at 680 °C with a Shimadzu TOC-V analyser connected in line with a TNM1 measuring unit. Alongside with water samples conductivity, temperature and depth (CTD; SeaBird SBE911 plus), and dissolved oxygen (SeaBird SBE43), fluorescence of chlorophyll (SeaPoint SCF), and turbidity (SeaPoint STM) were measured. CTD conductivity was calibrated with water samples taken from the rosette and analysed on board with a Guildline 8410-A Portasal salinometer. Samples for dissolved oxygen (O2) determination were analysed on board by the Winkler potentiometric method. The chlorophyll (Chl) fluorescence sensor was calibrated with water samples taken at 4 depths in the photic layer which were estimated fluorometrically by means of a Turner Designs bench fluorometer 10-AU, Horizon 2020 (H2020), grant/award no. 817806: Sustainable management of mesopelagic resources; Ministerio de Ciencia e Innovación (MICINN), grant/award no. CTM2015-69392-C3: Constraining organic carbon fluxes in an eastern boundary upwelling ecosystem (NW Africa): the role of non-sinking carbon in the context of the biological pump; Ministerio de Ciencia e Innovación (MICINN), grant/award no. PID2019-109084RB-C21: Biogeochemical impact of mesoscale and sub-mesoscale processes along the life history of cyclonic and anticyclonic eddies: plankton variability and productivity, Peer reviewed

GTEx PEMT-HMT correlation all tissues full labelled plots., Peer reviewed

GTEx NNMT-HMT correlation all tissues full labelled plots., Peer reviewed

TCGA NNMT-HMT correlation all cancers full labelled plots., Peer reviewed

CCLE NNMT-HMT correlation all cancers full labelled plots., Peer reviewed

Contents: S1 Geographical description of the German Part of the Elbe River basin – S1.-- S2 Drought indices – S9.-- S3 Aspects of the socio-economic drought impacts – S13.-- References, Peer reviewed