BUSCADOR RECOLECTA

This is the LMF version of the Apertium bilingual dictionary for English and Catalan languages. Bilingual LMF dictionaries were generated from Apertium bilingual dix files. For each Apertium bilingual correspondence, the corresponding source and target monolingual entries (LexicalEntry) were generated in addition to the bilingual correspondence (SenseAxis) element. Apertium is a free/open-source machine translation platform, initially aimed at related-language pairs but recently expanded to deal with more divergent language pairs (such as English-Catalan). The platform provides: a language-independent machine translation engine; tools to manage the linguistic data necessary to build a machine translation system for a given language pair and linguistic data for a growing number of language pairs.

This is the LMF version of the Apertium bilingual dictionary for English and Galician languages. Apertium is a free/open-source machine translation platform, initially aimed at related-language pairs but recently expanded to deal with more divergent language pairs (such as English-Catalan). The platform provides: a language-independent machine translation engine; tools to manage the linguistic data necessary to build a machine translation system for a given language pair and linguistic data for a growing number of language pairs.

This is the LMF version of the Apertium bilingual dictionary for English and Spanish languages. Bilingual LMF dictionaries were generated from Apertium bilingual dix files. For each Apertium bilingual correspondence, the corresponding source and target monolingual entries (LexicalEntry) were generated in addition to the bilingual correspondence (SenseAxis) element. Apertium is a free/open-source machine translation platform, initially aimed at related-language pairs but recently expanded to deal with more divergent language pairs (such as English-Catalan). The platform provides: a language-independent machine translation engine; tools to manage the linguistic data necessary to build a machine translation system for a given language pair and linguistic data for a growing number of language pairs.

This is the LMF version of the Apertium bilingual dictionary for Esperanto and English languages. Bilingual LMF dictionaries were generated from Apertium bilingual dix files. For each Apertium bilingual correspondence, the corresponding source and target monolingual entries (LexicalEntry) were generated in addition to the bilingual correspondence (SenseAxis) element. Apertium is a free/open-source machine translation platform, initially aimed at related-language pairs but recently expanded to deal with more divergent language pairs (such as Esperanto-English). The platform provides: a language-independent machine translation engine; tools to manage the linguistic data necessary to build a machine translation system for a given language pair and linguistic data for a growing number of language pairs.

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(A, B) Parental (Par), and Ndufs4−/− RAW 264.7 cells were left untreated or treated with LPS (100 ng/ml). Supernatants were collected at 8 hours for measurement of cytokine concentrations by ELISA (A). Cells were collected at 4 hours for quantification of cytokine transcripts using real-time PCR (expressed as fold increases versus untreated parental cells) (B). (C) Representative flow cytometry plots (left) showing phagocytosis of FITC labeled heat killed E. coli (HKEC) and bar graph representing % of fluorescent cells and the MFI values (right). Ctrl, control. Ns, not significant; *, P <0.05; **, P <0.01; ***, P<0.005; ****, P<0.001. Each point represents a biological replicate. In all experiments, 4 to 5 different Ndufs4−/− clones were used. Data are shown as the mean ± SEM., Peer reviewed

Native gels were incubated with NADH (as a substrate), and nitro blue tetrazolium (NBT, as the electron acceptor). CI activity is shown in purple., Peer reviewed

1D-BNE showing OXPHOS complexes I to IV (CI-CIV) and SCs. Western blot analysis was performed using antibodies against CI (NDUFA9), CIII (Core 2), CIV (COX5A), and CV (ATP5A). Asterisks (*) indicate lower molecular weight SC. Loading control, CII subunit SDHA., Peer reviewed

(A) Activities of MRC complex (I–IV) were assayed spectrophotometrically, and the results were normalized to citrate synthase (CS) activity in mitochondria isolated from parental (Par) and Ndufs4−/− RAW 264.7 cells. (B) Left panel, a representative experiment showing OCR in RAW 264.7 sublines before and after the sequential addition of oligomycin (2.6 μM), FCCP (1 μM), and a combination of rotenone (Rot) and antimycin A (AA) (1 μM). Right panel, basal respiration, maximal respiration, and ATP production. Ns, not significant; *, P <0.05; **, P <0.01; ***, P<0.005; ****, P<0.001. Each point represents a biological replicate. Data are shown as the mean ± SEM., Peer reviewed

(A) Structural model for ovine (Ovis aries) mitochondrial complex I (CI, Ref: 5LNK) [17] displaying the location of the NDUFS4 subunit (colored in black). UCSF ChimeraX (v1.5) [18] was used for visualization. (B) General Cas9 HITI strategy to ablate Ndufs4. Cas9 produces a double stranded DNA break at specific target sequences within the first exon of Ndufs4. Cas9 also excises the HITI donor by cleaving the same target sequence flanking the blasticidin cassette to be inserted. The excised HITI donor is ligated into the genomic site through the NHEJ pathway. The forward integration depicted in the scheme is “locked” and cannot be processed further. A reverse integration could be corrected by continuous excision/repair cycles in virtue of flanking target sites reconstitution. Grey pentagons represent Cas9/gRNA target sequences. Black lines within pentagons indicate Cas9 cleavage sites. (C) Whole cell lysates from each resultant knockout cell line were analyzed by Western blotting using antibodies against Ndufs4 or β-actin (loading control). Correctly targeted clones were named Ndufs4−/− followed by a serial number; Par, parental cell line., Peer reviewed