BUSCADOR RECOLECTA

The item is made of 2 files, of which 1 is the dataset and the other include a small description of the measured variables.-- Dataset contributed to the Project Carbochange.-- More information in http://hdl.handle.net/10261/115861, This dataset gathers discrete measurements of CO2 (pH and alkalinity) and hydrographic variables (salinity, temperature, dissolved oxygen, nitrate, phosphate and silicate) obtained during the cruise OVIDE 2010 carried out from 8 to 30 June 2010. pH was measured spectrophotometrically following the Clayton and Byrne (1993). This method consists on adding a dye solution to the seawater sample, so that the ratio between two absorbances at two different wavelengths is proportional to the sample pH. Alkalinity was measured using an automatic potentiometric titrator Titrando 809 Metrohm, with a Metrohm 6.0232.100 combination glass electrode and a Pt-1000 probe for temperature measurement following the methodology given by Pérez and Fraga (1987). Dissolved oxygen was analyzed following the widely applied Winkler method. Determinations of nitrate, phosphate and silicate were carried out following methods described by Hansen and Grassoff (1983) Salinity and Temperature were recorded with a CTD probe, French OVIDE Project supported by CNRS, and CARBOCHANGE, No

Open access. Please, contact corresponding author in case of doubts, Description: The Malaspina2010_chlorophyll.xlsx file contains the fluorimetric chlorophyll a data of the circunnavigation expedition Malaspina 2010, which took place between 14/12/2010 and 14/07/2011 on board the BIO Hespérides. The data presented here were obtained between 16/12/2010 and 11/07/2011. The date and position of the sampling stations are listed in the third sheet of the file. Methods: Water samples for fluorimetric chlorophyll a (Chl a) determination were collected from 3 m depth with a 30-liter Niskin bottle and from selected depths between 10 and 200 m with a Rosette of 24 10-liter Niskin bottles attached to a CTD probe. Chl a determination was carried out as described in Estrada et al. (2012). Briefly, between 200 and 500 cm3 of seawater were filtered through GF/F glass fiber filters that were subsequently frozen at -20°C and, after a minimum of 6 hours, introduced in vials with acetone 90% and left for 24 hours in the dark, at 4ºC. The Chl a concentration in the acetonic extracts was measured with a Turner Designs fluorimeter calibrated with a pure Chl a standard (Sigma-Aldrich); no phaeopigment correction was applied. Size-fractionated analyses of Chl a were performed for samples from surface, the 20% surface PAR and the deep chlorophyll maximum. The analyses were carried out by sequential filtration of a 500 cm3 of seawater through Poretics (polycarbonate) membrane filters of pore sizes 20 μm, 2 μm and 0.2 μm, which were subsequently treated as the GF/F ones (Estrada, 2012), This work was supported by Consolider-Ingenio 2010, CSD2008-00077 of the former Spanish Ministerio de Ciencia e Innovación (ES) (now www.ciencia.gob.es) and the Consejo Superior de Investigaciones Científicas (CSIC) (ES), Authors; Methods; Station positions; leg 1; leg 2; Legs 3-4; leg 5; leg 6; leg 7; References, Peer reviewed

Description: The Malaspina2010_chlorophyll.xlsx file contains the inorganic nutrient concentration data of the circunnavigation expedition Malaspina 2010, which took place between 14/12/2010 and 14/07/2011 on board the BIO Hespérides. The date and position of the sampling stations are listed in the “Data” sheet of the file. Methods: Water samples for measurement of the concentration of nitrate, nitrite (nitrate + nitrite in leg 1), phosphate and silicate were collected from 3 m depth with a 30-liter Niskin bottle and from selected depths between 10 and 200 m with a Rosette of 24 10-liter Niskin bottles attached to a CTD probe. Inorganic nutrient concentrations were determined by means of a Skalar autoanalyzer, following the standard spectrophotometric procedures described in Grasshoff et al. (1999) and Blasco et al. (2012); in leg 1, nitrite was not determined separately and phosphate was measured using a manual method (Vidal et al. 2012), This work was supported by Consolider-Ingenio 2010, CSD2008-00077 of the former Spanish Ministerio de Ciencia e Innovación (ES) (now www.ciencia.gob.es) and the Consejo Superior de Investigaciones Científicas (CSIC) (ES), Peer reviewed