Resultados de investigación

We thank C. L. Thomas for the preparation of the KC construct and advice, K. J. Verhey for the pcDNA3-HA-KLC-HR and pcDNA3-HA-KLC-TPR plas- mids, D. Badford and J. Yang for the Pp5 construct, and V. Cavalli for the SyD/JIP-3 cDNA and antibody. We thank D. Worth for the cloning of the KLC1 constructs, A. Nicol for the Mathematica workbook used for tracking data analysis, and P. Fitzjohn for the structure prediction analysis of KIM and KLC. We are grateful to S. Tooze, A. Behrens, and members of the Molecular Neuropathobiology laboratory for critical reading of the manuscript. This work was supported by Cancer Research UK., Kinase D-interacting substrate of 220 kDa/ankyrin repeat-rich membrane spanning (Kidins220/ARMS) is a conserved membrane protein mainly expressed in brain and neuroendocrine cells, which is a downstream target of the signaling cascades initiated by neurotrophins and ephrins. We identified kinesin light chain 1 (KLC1) as a binding partner for Kidins220/ARMS by a yeast two-hybrid screen. The interaction between Kidins220/ARMS and the kinesin-1 motor complex was confirmed by glutathione S-transferase-pull-down and coimmunoprecipitation experiments. In addition, Kidins220/ARMS and kinesin-1 were shown to colocalize in nerve growth factor (NGF)-differentiated PC12 cells. Using Kidins220/ARMS and KLC1 mutants, we mapped the regions responsible for the binding to a short sequence of Kidins220/ARMS, termed KLC-interacting motif (KIM), which is sufficient for the interaction with KLC1. Optimal binding of KIM requires a region of KLC1 spanning both the tetratricopeptide repeats and the heptad repeats, previously not involved in cargo recognition. Overexpression of KIM in differentiating PC12 cells impairs the formation and transport of EGFP-Kidins220/ARMS carriers to the tips of growing neurites, leaving other kinesin-1 dependent processes unaffected. Furthermore, KIM overexpression interferes with the activation of the mitogen-activated protein kinase signaling and neurite outgrowth in NGF-treated PC12 cells. Our results suggest that Kidins220/ARMS-positive carriers undergo a kinesin-1-dependent transport linked to neurotrophin action.

Dades relatives al nombre de cerques al catàleg de fons modern del CRAI de la UB durant el curs 2006-2007. Inclou en format csv les dades mensuals desglossades per tipus de cerca i per la ubicació des d'on s'ha fet la consulta.

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[EN] Programa del Congreso Internacional “Western Europe in the First Millennium BC: Crossing the divide”, organizado por Tom Moore y Xosé-Lois Armada y celebrado en el Departamento de Arqueología de la Universidad de Durham, Reino Unido (23-25 de noviembre de 2007)., [ES] Programme of the International conference “Western Europe in the First Millennium BC: Crossing the divide”, organized by Tom Moore and Xosé-Lois Armada and held at the Department of Archaeology, Durham University, UK (November 23-25th, 2007).

The ALCUDIA transect is a 230 km long, vertical incidence deep seismic reflection profile acquired in 2007. The acquisition parameters, 35 m station spacing, 70 m Vibration Point spacing resulted in a 60-90 fold high resolution seismic reflection image. A 20 s long Vibroseis sweep with frequencies between 8-80 Hz was recorded by a 400 station recording cable and 14 km long split spread configuration., ALCUDIA Normal Incidence (ALCUDIA-NI) is a NE-SW oriented vertical incidence deep seismic reflection transect in the SW of Spain that aimed to image the Variscan Iberian Massif. This transect sampled the Central Iberian Zone and was designed to be the northward continuation of the IBERSEIS-NI seismic profile. The processed image shows a weakly reflective upper crust, a thick, highly reflective and laminated lower crust, and a flat Moho located at 10 s TWTT (30 km depth). The transect can be divided into three segments with different structural styles in the lower crust. In the central segment, the lower crust is imaged by regular, horizontal and parallel reflectors, whereas in the northern and southern segments it displays oblique reflectors interpreted as an important thrust (north) and tectonic wedging involving the mantle (south). The ALCUDIA-NI shows that in an intracontinental orogenic crust, far from the suture zones, the upper and lower crust may react differently to shortening in different sectors, which is taken as evidence for decoupling. The interpreted structures, as deduced from surface geology and the seismic image, show that deformation was distributed homogeneously in the upper crust, whereas it was concentrated in wedge/thrust structures at specific sectors in the lower crust., Spanish Ministry of Science and Innovation, CGL2004-04623/BTE, CSD2006-00041.-- Ministerio de economía y competitividad, CGL2007-63101/BTE.-- Ministerio de economía y competitividad; Fondos Europeos de desarrollo regional (FEDER), CGL2011-24101.-- Junta de Castilla-La Mancha.--, Peer reviewed

RODA-II Cruise (29HE20070201) carried out on the Research Vessel Hespérides in 2007, Oceanic eddies and atmospheric deposition: influence on particle flows and remineralization in the water column. Study of marine eddies and their relationship with cyclones and anticyclones. They have made diverse experiments, to analyze the lethal thresholds of complex mixtures of organic pollutants (PCBs, PAHs ) on natural communities of phytoplankton. Experiments related to ultraviolet radiation, PAHs and Net community production have also been carried out during the cruise