Resultados de investigación

This PDF file includes: Supplementary Text: Fig. S1. Comparison of the phosphoproteomes of two AML cell lines. Fig. S2. Examples of substrate group enrichment. Fig. S3. Comparison of the different enrichment strategies. Fig. S4. Results of KSEA based on substrate groups that were defined by a common motif. Fig. S5. Characterization of the effects of PI3K inhibitors in P31/Fuj and Kasumi-1 cell lines. Fig. S6. Overview of phosphopeptide quantification in P31/Fuj and Kasumi-1 cell lines treated with PI3K and mTOR inhibitors. Fig. S7. PCA of phosphoproteomics data obtained from experiments in which P31/Fuj and Kasumi-1 cells were treated with AZ123, Ku-794, or PI-103. Fig. S8. KSEA of the phosphoproteomes of inhibitor-treated cells based on substrate groups containing given phosphorylation motifs. Fig. S9. DNA-PK is activated as a result of the initiation of apoptosis induced by PI3K and mTOR inhibitors. Fig. S10. Differentiating between on-target and off-target effects of PI3K and mTOR inhibitors. Fig. S11. Verification that the primary AML cells were metabolically active and had functional kinase signaling networks. Fig. S12. Unsupervised hierarchical classification of primary AML cells based on enrichment data obtained with KSEA. Fig. S13. Clusters of substrate groups found together in primary AML cells. Table S1. Characteristics of AML primary samples. Table S2. Results of linear regression model using the predefined substrate groups shown in Fig. 4. Table S3. Results of linear regression model with the newly defined phosphorylated peptide groups shown in Fig. 5. Table S4. Antibodies used for Western blotting experiments. Other Supplementary Material for this manuscript includes the following: (available at www.sciencesignaling.org/cgi/content/full/6/268/rs6/DC1) Data set 1 (Microsoft Excel format). Phosphorylated peptides in cell lines. Data set 2 (Microsoft Excel format). Phosphorylated peptides in primary cells. Data set 3 (Microsoft Excel format). Details of KSEA in primary cells., Peer reviewed

This dataset provides information on the diversity and abundance of fish collected during the SPURS-MIDAS survey in the Central North Atlantic. This was an oceanographic survey in which opportunistic plankton sampling was carried out twice a day. At night, surface waters were sampled with a Neuston net. During the day the epipelagic layer was sampled with a Bongo net, MIDAS‐6. Productos SMOS de salinidad oceánica y humedad del suelo. Mejoras y demostración de aplicaciones” (AYA2010‐22062‐C05‐01). SPURS experiment (Salinity Processes in the Upper ocean Regional Study), Peer reviewed

Peer Reviewed

13 files, This dataset gathers discrete measurements of CO2 (pH and alkalinity) and hydrographic variables (salinity, temperature, dissolved oxygen, nitrate, phosphate and silicate) in 10 cruises between 2005 and 2007 in the GIFT Time Series Stations located at the Strait of Gibraltar. pH was measured spectrophotometrically following the Clayton and Byrne (1993). This method consists on adding a dye solution to the seawater sample, so that the ratio between two absorbances at two different wavelengths is proportional to the sample pH. Alkalinity was measured using an automatic potentiometric titrator Titrando 794 Metrohm, with a Metrohm 6.0232.100 combination glass electrode and a Pt-1000 probe for temperature measurement following the methodology given by Pérez and Fraga (1987). Dissolved oxygen was analyzed following the widely applied Winkler method. Determinations of nitrate, phosphate and silicate were carried out following methods described by Hansen and Grassoff (1983) Salinity and Temperature were recorded with a CTD probe, Data are part of the EC-funded Integrated Project CARBOOCEAN - Marine carbon sources and sinks assessment Contract no. 511176 (GOCE), more details available at http://www.carboocean.org/, CARBOGIB2005.exc.xlsx.-- Metadata_CARBOGIB2005_Dec2005.xls.-- Metadata_CARBOGIB2005_Dec2006.xls.-- Metadata_CARBOGIB2005_July2007.xls.-- Metadata_CARBOGIB2005_March2006.xls.-- Metadata_CARBOGIB2005_May2005.xls.-- Metadata_CARBOGIB2005_May2006.xls.-- Metadata_CARBOGIB2005_May2007.xls.-- Metadata_CARBOGIB2005_Nov2006.xls.-- Metadata_CARBOGIB2005_Nov2007.xls.-- Metadata_CARBOGIB2005_Sep2005.xls.-- Discrete_measurement_methodology.docx.-- Readme_discrete_2007, Peer reviewed

The item is made of 2 files, of which 1 is the dataset and the other includes a small description of the measured variables.-- Dataset contributed to the Project Carbochange, This dataset gathers discrete measurements of CO2 (pH and alkalinity) and hydrographic variables (salinity, temperature, dissolved oxygen, nitrate, phosphate and silicate) obtained during the cruise P3A22008. carried out in the Strait of Gibraltar from 6 to 12 October 2008, pH was measured spectrophotometrically following the Clayton and Byrne (1993). This method consists on adding a dye solution to the seawater sample, so that the ratio between two absorbances at two different wavelengths is proportional to the sample pH. Alkalinity was measured using an automatic potentiometric titrator Titrando 794 Metrohm, with a Metrohm 6.0232.100 combination glass electrode and a Pt-1000 probe for temperature measurement following the methodology given by Pérez and Fraga (1987). Dissolved oxygen was analyzed following the widely applied Winkler method. Determinations of nitrate, phosphate and silicate were carried out following methods described by Hansen and Grassoff (1983) Salinity and Temperature were recorded with a CTD probe, Proyecto CARBOOCEAN (511176GOCE), y proyectos del Ministerio de Ciencia e Innovación (CTM2005/01091-MAR y CTM2008-05680-C02-01), Discrete_measurement_2008.csv.-- Metadata_discrete_measurements_P3A2.xlsx.-- Readme_Discret_measurement_2008.txt, Peer reviewed

The item is made of 2 files, of which 1 is the dataset and the other include a small description of the measured variables.-- Dataset contributed to the Project Carbochange, This dataset gathers discrete measurements of CO2 (pH and alkalinity) and hydrographic variables (salinity, temperature, dissolved oxygen, nitrate, phosphate and silicate) obtained during the cruise MOC2Austral carried out from 8 February to 10 March 2010. pH was measured spectrophotometrically following the Clayton and Byrne (1993). This method consists on adding a dye solution to the seawater sample, so that the ratio between two absorbances at two different wavelengths is proportional to the sample pH. Alkalinity was measured using an automatic potentiometric titrator Titrando 809 Metrohm, with a Metrohm 6.0232.100 combination glass electrode and a Pt-1000 probe for temperature measurement following the methodology given by Pérez and Fraga (1987). Dissolved oxygen was analyzed following the widely applied Winkler method. Determinations of nitrate, phosphate and silicate were carried out following methods described by Hansen and Grassoff (1983) Salinity and Temperature were recorded with a CTD probe, Spanish Projects GHG4MOC (CTM2009-07574-E) and MOC2 (CTM2008-06438-C02-02/MAR) supported by Ministerio de Ciencia e Innovación, and CARBOCHANGE (264879), Clivar_A21_2010.exc.csv.-- Metadata_MOC2Austral_2010.xls.-- Readme_Clivar_A21_2010.txt, Peer reviewed

Dataset contributed to the Project Carbochange.-- More information in http://hdl.handle.net/10261/93331, This dataset gathers discrete measurements of CO2 (pH and alkalinity) and hydrographic variables (salinity, temperature, dissolved oxygen, nitrate, phosphate and silicate) obtained during the cruise WOCE Section A05 carried out from 14 July to 15 August 1992. The pH was measured potentiometrically in the NBS scale with a combined glass electrode associated to a thermo-compensator and referred to 15ºC (Perez and Fraga, 1987a). The method had a shipboard precision of 0.005 units, based on 184 replicate analysis corresponding to two different oceanographic bottles fired at the same depth at each station (Ríos and Rosón, 1996). AT measurements were made by titration of seawater with potentiometric endpoint detection (Perez and Fraga, 1987b), reaching a precision of ±1 µmol kg-1. Dissolved oxygen was analyzed following the widely applied Winkler method. Determinations of nitrate, phosphate and silicate were carried out following methods described by Hansen and Grassoff (1983) Salinity and Temperature were recorded with a CTD probe, Instituto Español de Oceanografía y Comisión Interministerial de Ciencia y Tecnología (CICYT), Hesperides_1992.csv.-- Readme_Hesperides_1992.txt, Peer reviewed

The item is made of 2 files, of which 1 is the dataset and the other include a small description of the measured variables.-- Dataset contributed to the Project Carbochange, This dataset gathers discrete measurements of CO2 (pH and alkalinity) and hydrographic variables (salinity, temperature, dissolved oxygen, nitrate, phosphate and silicate) obtained during the cruise FICARAM-XV carried out along the A17 section from 20 March to 2 May 2013. pH was measured spectrophotometrically following the Clayton and Byrne (1993). This method consists on adding a dye solution to the seawater sample, so that the ratio between two absorbances at two different wavelengths is proportional to the sample pH. Alkalinity was measured using an automatic potentiometric titrator Titrando 809 Metrohm, with a Metrohm 6.0232.100 combination glass electrode and a Pt-1000 probe for temperature measurement following the methodology given by Pérez and Fraga (1987). Dissolved oxygen was analyzed following the widely applied Winkler method. Determinations of nitrate, phosphate and silicate were carried out following methods described by Hansen and Grassoff (1983) Salinity and Temperature were recorded with a CTD probe, Ministry of Economy and Competitiveness, CATARINA project (CTM2010-17141); European Commission, project Carbochange, Ficaram_XV_2013.exc.csv.-- Metadata_FICARAM-XV-2013.xls.-- Readme_Hesperides_2013.txt, No

The item is made of 2 files, of which 1 is the dataset and the other include a small description of the measured variables.-- Dataset contributed to the Project Carbochange.-- More information in http://hdl.handle.net/10261/115854, This dataset gathers discrete measurements of CO2 (pH and alkalinity) and hydrographic variables (salinity, temperature, dissolved oxygen, nitrate, phosphate and silicate) obtained during the cruise OVIDE 2006 carried out from 23 May to 26 June 2006. pH was measured spectrophotometrically following the Clayton and Byrne (1993). This method consists on adding a dye solution to the seawater sample, so that the ratio between two absorbances at two different wavelengths is proportional to the sample pH. Alkalinity was measured using an automatic potentiometric titrator Titrando 809 Metrohm, with a Metrohm 6.0232.100 combination glass electrode and a Pt-1000 probe for temperature measurement following the methodology given by Pérez and Fraga (1987). Dissolved oxygen was analyzed following the widely applied Winkler method. Determinations of nitrate, phosphate and silicate were carried out following methods described by Hansen and Grassoff (1983) Salinity and Temperature were recorded with a CTD probe, Spanish Project (CTM2007-28896-E), French OVIDE Project supported by CNRS, and CARBOOCEAN (511176GOCE), OVIDE_2006.exc.csv.-- Metadata__Ovide_2006.xls.-- Readme_Ovide_2006.txt, Peer reviewed

The item is made of 2 files, of which 1 is the dataset and the other include a small description of the measured variables.-- Dataset contributed to the Project Carbochange, This dataset gathers discrete measurements of CO2 (pH and alkalinity) and hydrographic variables (salinity, temperature, dissolved oxygen, nitrate, phosphate and silicate) obtained during the cruise OVIDE 2008 carried out from 6 June to 11 July 2008. pH was measured spectrophotometrically following the Clayton and Byrne (1993). This method consists on adding a dye solution to the seawater sample, so that the ratio between two absorbances at two different wavelengths is proportional to the sample pH. Alkalinity was measured using an automatic potentiometric titrator Titrando 809 Metrohm, with a Metrohm 6.0232.100 combination glass electrode and a Pt-1000 probe for temperature measurement following the methodology given by Pérez and Fraga (1987). Dissolved oxygen was analyzed following the widely applied Winkler method. Determinations of nitrate, phosphate and silicate were carried out following methods described by Hansen and Grassoff (1983) Salinity and Temperature were recorded with a CTD probe, French OVIDE Project supported by CNRS, and CARBOOCEAN (511176GOCE), Ovide_2008.exc.csv.-- Metadata_Ovide_2008.xls.-- Readme_Ovide_2008.txt, Peer reviewed