Resultados de investigación

20 files, Mussels (Mytilus galloprovincialis) were exposed during 24 hours to a waterborne infection with 10E8 CFU/ml Vibrio splendidus (reference strain LGP32) in the tank water. Five biological replicates were used for each infected and control conditions, APDAPD3401230109.mgf.-- APDAPD3401230109.raw.-- APDAPD3401230110.mgf.-- APDAPD3401230110.raw.-- APDAPD3401230111.mgf.-- APDAPD3401230111.raw.-- APDAPD3401230112.mgf.-- APDAPD3401230112.raw.-- APDAPD3401230113.mgf.-- APDAPD3401230113.raw.-- APDAPD3401230114.mgf.-- APDAPD3401230114.raw.-- APDAPD3401230115.mgf.-- APDAPD3401230115.raw.-- APDAPD3401230116.mgf.-- APDAPD3401230116.raw.-- README.txt.-- checksum.txt.-- peptides.pep.xml, Peer reviewed

Two datasets are provided: general data on breeders (onset and end of the pre-breeding period) and newborn general information and biochemical data, Seahorses (Hippocampus spp.) are exceptional marine species considering their reproductive patterns and other features. Due to the iconic characteristics of these fishes, aquarium trade and research efforts have increased in the last years. Consequently, novel rearing techniques have been developed; however, there is a need for improvements on a series of issues, namely reproduction success enhancement. The tropical species Hippocampus reidi is the most traded seahorse but many aspects of breeding and its impact on the quality of neonates are still poorly understood. In the present study, we assessed the effects of two pre-breeding diets on newborn quality and viability considering biochemical characteristics, energetic status and ultrastructural aspects of muscular tissue. During the whole pre-breeding season (5 months), the breeders were fed on one of the following diets: M0 (adult non-enriched Artemia) and M5 (adult non-enriched Artemia + mysidaceans). From the onset of the reproduction period, all breeders were fed for 6 months on diet M5. Breeding success and energetic status (ATP, total adenylic nucleotides, AEC and NAD) of newborns resulted considerably enhanced in treatment M5. However, initial differences in neonates quality did not affect further newborn performance (survival and growth until day 7 after male’s pouch release) while gaining access to high-quality preys (copepods). Besides, morphological alterations in muscle tissue were not observed. The reproduction in the species followed a capital–income continuum pattern characterized by an initial mixed capital-income period (until 70-100 days since the onset of the breeding season) followed by an income breeding period with progressive exhaustion of body reserves, especially in M0-newborns. Interestingly, the effects of pre-breeding diets were also noticed in the second half of the breeding period. Our results seemed to indicate that the requirements in essential fatty acids in H. reidi are lower than in other seahorse species (e.g., H. guttulatus). Globally, the results achieved revealed that high-quality pre-breeding diets enhanced reproduction success and would likely result advantageous to improve newborn endurance in conditions of moderate starvation or sub-optimal feeding, Peer reviewed

6 figures, 3 tables, Supplementary material for the article https://doi.org/10.1016/j.marenvres.2021.105315, Figure S1. Results of Vtg mRNA expression in females after normalization process with a different number of reference genes.-- Figure S2. Results of Vtg mRNA expression in males after normalization process with a different number of reference genes.-- Figure S3. Individual observation of RT-qPCR data for female and male different Vtg domains normalized with different number of reference genes.-- Figure S4. Bioanalizer profiles of three samples of RNA selected to assess RNA quality.-- Figure S5. Melt curve analysis of reference genes and vitellogenin primer pairs.-- Figure S6. Results of 1% agarose gel electrophoresis of PCR product using all primer pairs tested.-- Table S1. Equations of standard curves for primers pair efficiency.-- Table S2. Power analysis showing the effect size that could be confidently detected (% change in comparison with control values) in our RT-qPCR analyses results using a sample size of 3, and the averaged observed standard deviation (SD) in our samples.-- Table S3. Results of Two-Way ANOVA performed in females and males respectively to evaluate the effect of factor "time” (t4 and t24), factor “chemical” (C, SC and EE2) and the interaction of the two factors on Vtg mNRA normalized expression levels with different number of reference genes.-- Zip mmc2. Sequences.-- Zip mmc3. Alignments, Peer reviewed

5 files, Supplementary data for the article https://doi.org/10.1098/rstb.2020.0165, MGR_conserved_miRNAs.fas.-- PFU_conserved_miRNAs.fas.-- RPH_conserved_miRNAs.fas.-- SBR_conserved_miRNAs.fas.-- TGR_conserved_miRNAs.fas, Peer reviewed

1 file, Supplementary information for the article https://doi.org/10.1016/j.scitotenv.2021.146570, Figure S1. Distribution of samples in the Argentine Basin by month.-- Figure S2. Number of samples by year and layer.-- Figure S3. Vertical profile of Cant (red) and xc[CO3 2-] (black) for all the samples available in the Argentine Basin over the time period 1972-2019.-- Figure S4. Mean water mass natural fraction of dissolved inorganic carbon (DICnat, μmol kg-1) versus atmospheric CO2 concentration (ppm) in the Argentine Basin.-- Figure S5. Mean water mass property versus atmospheric CO2 concentration (ppm) in the Argentine Basin.-- Table S1. List of selected cruises in the Argentine Basin (western South Atlantic).-- Table S2: Observed trends in Argentine Basin water masses.-- Table S3: Observed trends in Argentine Basin water masses versus time (years), Peer reviewed

18 pages, Supporting information for the article https://doi.org/10.1016/j.scitotenv.2021.145020, Peer reviewed

40 pages, Supplementary information for the article https://doi.org/10.1038/s41598-021-02328-6, Approach.-- Standardization of indicators,-- Calculation of index values.-- Socioeconomic indicators and factors.-- Institutional Indicators and Factors.-- Resilience factors summary.-- Index performance.-- Additional analyses.-- Bibliography, Peer reviewed

1 .zip file containing aerial photographs (.pdf files)., Scans of paper contact copies of aerial photographs with the wax pencil marks by the soil surveyors in 1975. The survey area is the land irrigated by the Canal of Flumen at the left side of the River Flumen in the province of Huesca, Spain., Peer reviewed

We have organized the data in three files: • File "Augerings 1975 Monegros.docx", a list of the 519 soil samples taken by hand-auger in 1975 at 183 sites. The list shows the refernce of the sampling site, the depth of each sample, the electrical conductivity at 1:5 dilution, and the pH at 1:1 dilution; • File "Coordinates 1975 Monegros.docx", the UTM coordinates of the sampling sites; and • File "Marked contacts CETFA Monegros1972 and diagram.zip”, the scannings in pdf format of the 176 contacts aerial photographs from a flight of 1972 that have marks of the surveyors for the localization of the sampling sites. This zip file also contains the flight diagram in pdf format., Salinity is a time-variable characteristic trait of the soils, as is well known in the irrigated lands. Tracking the variation of soil salinity along time is a way to evaluate the effects of irrigation in agricultural lands or in their surroundings. Such evaluations can enable warning and even implementing corrective actions if salinity underwent undesirable evolution. The data presented here come from a heritage report commissioned by IRYDA, the organism in charge of new irrigation projets within the Spanish Ministry of Agriculture., The time elapsed and the changes in the irrigation techniques in the district, where now is becoming majoritarian the pressurized application of water, reinforce the interest of this dataset for multitemporal evaluations with agronomical or environmental purposes., Peer reviewed

A set of historical tide gauge sea-level records from two locations in Santander (Northern Spain) and Alicante (Spanish Mediterranean coast) have been recovered from logbooks stored in national archives. Sea-level measurements have been digitized, quality-controlled and merged into three consistent sea-level time series (two in Alicante and one in Santander) using high-precision levelling information. The historical sea-level record in Santander consists of a daily time series spanning the period 1876-1924 and it is further connected to the record from the modern tide gauge station nearby, ensuring datum continuity up to the present. The sea-level recording in Alicante started in 1870, with daily averaged values until the 1920s and hourly afterwards, and the tide gauges at the two Alicante sites are still operating, thereby providing one of the longest tide gauge sea-level time series in the Mediterranean Sea. The long-term consistency and reliability of the new records are discussed based on comparison with nearby tide gauge time series., The research presented in this paper was partially funded by the Spanish Ministry of Science, Innovation and Universities (MOCCA, project number RTI2018-093941-B-C31) (M.M., A.A.) and the National Science Foundation CAREER award number 1455350 (S.T.)., Peer reviewed