Resultados de investigación

Additional file 2 Figure S1. Percentage of G-Actin in filaments. Percentage of G-Actin in filaments of different size for the three characteristic regimes., Ministerio de Ciencia, Innovación y Universidades Ministerio de Ciencia, Innovación y Universidades, Peer reviewed

Additional file 3 Figure S2. Dependence of speed of treadmiling with F-actin length. Plot of the dependence of the instantaneous speed on the filament size. Short filaments move faster than average. Long filaments move at the same speed in average., Ministerio de Ciencia, Innovación y Universidades, Peer reviewed

Additional file 4: Movie S2. Time-lapse movie of the system with F-actin and ACs. G-actin is labeled in green, ACs labeled in blue., Ministerio de Ciencia, Innovación y Universidades, Peer reviewed

Additional file 5 Figure S3. G-Actin, F-actin after incorporation of ACs. Number of G-Actin in the grid (green), G-actin as part of networks (orange) and ACs (blue) after incorporation of ACs into the system at t=5E6 iterations. The formation of networks is initial very fast, followed by a regime where incorporation of molecules into networks is gradually slowing down until equilibrium is reached., Ministerio de Ciencia, Innovación y Universidades, Peer reviewed

Additional file 6: Movie S3. Time-lapse movie of the system showing oscillations. Time-lapse movie of the system showing periodic assembly and disassembly of the actomyosin cortex (G-actin labeled in green, ACs labeled in red, Myosin labeled in red)., Ministerio de Ciencia, Innovación y Universidades, Peer reviewed

Additional file 7 Figure S4. Oscillations for different levels of Myosin. Oscillations in the number of G-actin in the cortex for conditions of (A) low and (B) high concentration of Myosin in the system. The corresponding Fourier transform for each oscillation is also shown., Ministerio de Ciencia, Innovación y Universidades, Peer reviewed

Additional file 8: Movie S4. Oscillations in D. Melanogaster basal follicle cells. Control conditions. Cells are stained with Lifeact-GFP., Ministerio de Ciencia, Innovación y Universidades, Peer reviewed

Additional file 9: Movie S5. Oscillations in D. Melanogaster basal follicle cells. Experimental conditions. Time-lapse movie of the D. Melanogaster basal follicle cells stained with Lifeact-GFP in conditions of over-expression of the two subunits of the integrin molecule., Ministerio de Ciencia, Innovación y Universidades, Peer reviewed

Additional file 10 Figure S5. Oscillations at lower resolution for different energies. Oscillations of the model at lower resolution, at different energies E=1 (left) and E=5 (right). When resolution is lowered, the lower part of the oscillation is filtered out and the increase in energy results in a net increase in the average levels of the actin in cortex, more similar to the effect observed experimentally (Fig. 6 F)., Ministerio de Ciencia, Innovación y Universidades (España), Peer reviewed

Additional file 11 Figure S6. Scheme of the framework. (A) Molecules diffuse freely in a three-dimensional space (cytoplasm) adjacent to a two-dimensional grid (inner plasma membrane) where molecules can attach. G-Actin (green) molecules in the grid interact and polymerize directionally to form F-Actin. ACs (blue) and Myosin (red) also interact with F-Actin to form networks of F-actin. (B) F-actin filament is formed by assembly at the barbed end (regulated by μ1) and disassembly at the pointed end (regulated by E1). (C) Linker formation of ACs and Myosin to F-Actin are regulated by μ2 and μ3, respectively. Release of ACs and Myosin is regulated by E2 and E3, respectively. (D-F) Shape of the potential function μi at a given time point for different values of (D) the reference potential μi,0, (E) the total G-actin molecules in the system Ni,0, and (F) the shape parameter γi., Ministerio de Ciencia, Innovación y Universidades (España), Peer reviewed