Resultados de investigación

Base de datos con información mensual y anual de pescado comercializado en las diferentes lonjas de Catalunya, Comunitat Valenciana, Murcia, Baleares y Andalucía. Datos mensuales de cantidad, precio medio y valor total por especie ., Esta base de datos se ha realizado a través del proyecto Digitalización y valorización de la pesca en el Mediterráneo español (DIGIPESCA,) financiado por el Ministerio de Agricultura, Pesca y Alimentación a través de los fondos NextGeneration UE.

Base de datos con información mensual y anual de pescado comercializado en las diferentes lonjas de Catalunya, Comunitat Valenciana, Murcia, Baleares y Andalucía. Datos mensuales de cantidad, precio medio y valor total por especie ., Esta base de datos se ha realizado a través del proyecto Digitalización y valorización de la pesca en el Mediterráneo español (DIGIPESCA,) financiado por el Ministerio de Agricultura, Pesca y Alimentación a través de los fondos NextGeneration UE.

Correlaciones de Pearson entre variables por países. Ligas europeas de fútbol. Temporada 2019-2020., Departamento de comunicación y publicidad, Correlaciones de Pearson entre variables por países. Ligas europeas de fútbol. Temporada 2019-2020.

Correlaciones, árbitros y componentes del juego. Ligas europeas de fútbol. 2019-2020, Departamento de comunicación y publicidad, Correlaciones, árbitros y componentes del juego. Ligas europeas de fútbol. 2019-2020

The relationship between scatter-hoarding rodents and oak species has been considered on a scale from antagonism to mutualism. Depending on the costs and benefits, the outcome of the relationship can be found at one extreme or the other. Costs have included destruction of acorns that occurs during predation, but not all acorns attacked lose their embryos. As representatives of the mutualistic end, we present two species with this behavior (Mus spretus and Apodemus sylvaticus). Representing the antagonistic extreme, we present a predatory species that destroys the embryo (Microtus arvalis). The objective of this study is to test the preferences of both rodent groups for acorns. The results showed that there is one acorn species (Quercus ilex) that is preferred by the three rodent species. This acorn species has high concentrations of nutrients, low concentrations of tannins and thin shell. These characteristics attract the attention of rodents that could carry these acorns. There are two other acorn species (Quercus suber and Quercus rubra) that are consumed with little intensity for having low concentrations of nutrients, high concentration of tannins and thick shell. These characteristics escaping predators although transportation is not guaranteed. These acorns would germinate and emerge under the trees that have produced them and increase intraspecific competition. Coincidence preferences shown by the three rodent species poses a risk for the oak species, since the recent arrival of the predator (antagonist) species in the study area could paralyze the dispersal process carried out by the other two mutualistic species through predation., Departamento de Ciencias Agroforestales

Sample preparation for the analysis of organic micropollutants in wastewater samples is commonly carried out by solid-phase extraction (SPE) procedures, which involve different manual laboratory operations. This conventional approach requires several hours of counter labour and entail the use of a lot of disposable material, and the subsequent contaminated non-recyclable plastic-residue production. In contrast, by coupling and automatizing the pre-treatment to the instrumental analysis most of that burden erases, sample size gets miniaturized and, thus, storage becomes freed-up. Even lab counters get cleared off from sample pre-treatment apparatus. However, method performance could get alter as a trade-off. This paper presents the results from a study in which methodology, including SPE online-coupled to UHPLC-MS/MS chromatography, was developed for multi-residue (58) determination of veterinary and pharmaceutical drugs in urban and piggery wastewater (influent and effluent to wastewater treatment plants (WWTPs)). Similarly, the direct injection (DI) of large volumes (hundreds of µL) of same matrix samples into the chromatographic system was optimized too. The performance of both automated methods was statistically compared with the classical off-line SPE. As dealing with trace analysis, suitable injection volumes for the alternative approaches were selected on the premise of low limits of quantification (MLQs). Under the selected conditions, validation parameters such as linearity range, method quantification limits, peak shape and carry over were determined. Usually more than 50 % of the analytes showed MLQs below 50 ng/L, for all matrices and methodologies, especially for DI. Real wastewater samples from a local urban WWTP and farm were analysed with all three tested methodologies. Determined concentrations and removal rates were statistically compared and turned out being quite similar. However, analysis under offline SPE and DI approaches provided a larger amount of information as they reached lower MLQs. Offline-SPE provided the worst precision among all., Instituto de Procesos Sostenibles, Departamento de Ingeniería Química y Tecnología del Medio Ambiente, Departamento de Química Analítica, Ministerio de Ciencia, Innovación y Universidades, EU Feder Programme, Consejeria de Educacion Junta de Castilla y Leon

Scenedesmus almeriensis microalgae–bacteria consortia were evaluated for the removal of a mixture of tetracycline (TET), ciprofloxacin (CIP), and sulfadiazine (SDZ) from the real liquid fraction of pig slurry in a pilot scale photobioreactor. After 15 days of operation, the reactor was spiked with a mixture of /L of each antibiotic. The experiment ran for 20 additional days. From the liquid phase, antibiotic removal were 77 ± 5 %, 90 ± 14 %, and 60 ± 27 % for TET, CIP, and SDZ, respectively. The antibiotics found in the solid phase were 979 ± 382 ng/g for TET and 192 ± 69 ng/g for SDZ; CIP was not detected in the biomass. The parameters analyzed before and after antibiotic addition showed that the antibiotics did not have a negative effect on the reactor biomass. The removal efficiencies of the analyzed parameters were 64.6 ± 0.6 % for TOC, 56.9 ± 0.6 % for IC, 63.9 ± 0.6 % for TN, 88.6 ± 0.9 % for N-NH4+, 64.9 ± 0.6 % for N-NO3-, and 30.1 ± 0.3 % for P-PO4 3- . This study demonstrated the good performance of microalgae-based technology for swine manure wastewater treatment, not only in terms of organic matter and nutrient removal, but also regarding the removal of antibiotics. The mass balance analysis of the entire process is presented. Additionally, the present study is a validation of previous laboratory scale batch studies operating in a quasi-continuous mode on veterinary antibiotics (VA) removal efficiencies and kinetics., Instituto de Procesos Sostenibles, Departamento de Ingeniería Química y Tecnología del Medio Ambiente, Departamento de Química Analítica, Ministerio de Ciencia, Innovación y Universidades, Consejeria de Educacion Junta de Castilla y Leon, EU Feder Programme

The mechanisms involved in the removal of a mixture of four veterinary antibiotics (VA) – tetracycline (TTC), ciprofloxacin (CPF), sulfadiazine (SDZ) and sulfamethoxazole (SMX) – in synthetic wastewater using microalgae–bacteria consortia (MBC) dominated by Scenedesmus almeriensis was studied at different initial concentrations of 1000, 500, 100 and 20 g/L per antibiotic. Ultra-high performance liquid chromatography and tandem mass spectrometry (UHPLC-MS/MS) were used to determine the removal of the VA for each mechanism. For a hydraulic retention time of 4 days, the overall removal of antibiotics by the MBC was 99.9% for TTC, 78.0% for CPF, 52.6% for SDZ and 5.0% for SMX. A pseudo-first order irreversible model was applied to best fit the experimental data. The degradation constant rates were 0.136 h−1 for TTC, 0.012 h−1 for CPF, 0.010 h−1 for SDZ and 0.0007 h−1 for SMX. Under all the evaluated conditions, CPF and TTC exhibited the highest removal efficiency. Biosorption was the main mechanism for all four antibiotics, followed by biodegradation in the cases of TTC and SDZ. CPF did not show removal via biodegradation. SMX did not show removal via hydrolysis or photolysis. This study (i) integrates and evaluates individually the mechanisms involved in VA removal using an MBC; (ii) determines an overall removal rate constant for a wide array of TTC, CPF, SDZ and SMX concentrations; and (iii) demonstrates the high removal capacity and potential use of microalgae as an ecofriendly wastewater treatment process., Instituto de Procesos Sostenibles, Departamento de Ingeniería Química y Tecnología del Medio Ambiente, Departamento de Química Analítica, Ministerio de Ciencia, Innovación y Universidades, Consejeria de Educacion Junta de Castilla y Leon, EU Feder Programme, Generalitat Valenciana, Spain

Despite the interest in the utilization of photobioreactors as an alternative wastewater treatment, the research about posterior recovery and valorization of nutrients accumulated in the biomass is still limited. This work compared several hydrolysis methods for the recovery of proteins and carbohydrates from the biomass grown in a photobioreactor treating swine wastewater. Ultrasound-assisted and microwave-assisted enzymatic hydrolysis at mild conditions and chemical methods at different temperatures (40, 60, 120ºC) were applied to the microalgae and bacteria biomass. Alkaline hydrolysis provided the greatest peptide recoveries, increasing with temperature up to a maximum of 81%, but with very small peptide sizes in all temperature range. Acid hydrolysis provided the highest carbohydrate recoveries (60.7% at 120ºC) but degraded proteins, even at mild temperatures. Protein degradation did not vary with temperature in each chemical hydrolysis, obtaining similar peptide sizes in all temperatures, while carbohydrate losses were higher at lower temperatures. Ultrasound-assisted enzymatic extraction recovered 43.6% of the initial proteins as large peptides (up to 135 kDa) with the highest peptide purity (46.7%). Microwave-assistance increased the carbohydrate solubilization of enzymatic hydrolysis, achieving yields of 73% of xylose, but with significant losses., Instituto de Procesos Sostenibles, Departamento de Ingeniería Química y Tecnología del Medio Ambiente, Ministerio de Ciencia, Innovación y Universidades, Consejeria de Educacion Junta de Castilla y Leon

The intensification of livestock activities lead to an increase in waste generation with high content of nutrients, as is the case of piggery wastewater. However, this type of residue can be used as culture media for algae cultivation in thin-layer cascade photobioreactors to reduce its environment impact and produce a valorizable algal biomass. Biostimulants were produced by enzymatic hydrolysis and ultrasonication of microalgal biomass, using membranes (Scenario 1) or centrifugation (Scenario 2) as harvesting methods. The co-production of biopesticides by solvent extraction was also evaluated using membranes (Scenario 3) or centrifugation (Scenario 4). The four scenarios were analyzed by a technoeconomic assessment estimating the total annualized equivalent cost and the production cost, i.e., the minimum selling price. Centrifugation provided biostimulants approximately 4 times more concentrated than membranes, but with higher expense due to the cost of the centrifuge (contribution of 62.2 % in scenario 2) and the electricity requirements. The biopesticide production resulted the highest contribution to investment cost in scenarios 3 and 4 (34 % and 43 % respectively). The use of membranes was also more advantageous to produce biopesticides, although it was 5 times more diluted than using centrifuge. The biostimulant production cost was 65.5 €/m3 with membranes and 342.6 €/m3 by centrifugation and the biopesticide production cost was 353.7 €/m3 in scenario 3 and 2,122.1 €/m3 in scenario 4. Comparing the treatment of 1 ha of land, the cost of the biostimulant produced in the four scenarios was lower than the commercial one (48.1 %, 22.1 %, 45.1 % and 24.2 % respectively). Finally, using membranes for biomass harvesting allowed economically viable plants with lower capacity and longer distance for biostimulant distribution (up to 300 km) than centrifuge (188 km). The algal biomass valorization for agricultural products production is an environmentally and economically feasible process with the adequate capacity of the plant and distribution distance., Instituto de Procesos Sostenibles, Departamento de Ingeniería Química. Universidad de Almeria, Departamento de Ingeniería Química y Tecnología del Medio Ambiente, Ministerio de Ciencia, Innovación y Universidades, Consejeria de Educacion Junta de Castilla y Leon