Resultados de investigación

1 zip file.-- Also available in https://github.com/aspillaga/TOA-ACTIVITY-MEE/tree/1.0, Data and R scripts for TOA-based activity estimation and behavioural segmentation from acoustic telemetry data. Contains the data and analyses supporting the manuscript "Inferring activity patterns of aquatic animals from single-receiver acoustic telemetry setups" published in Methods in Ecology and Evolution, Peer reviewed

Central sections from the 3DR (left half) and 8 Å–thick slabs (right half) contoured at 1.2σ above the mean density to highlight the RNA density (as shown in Fig 1H–K, lower row). In central sections, brigther shading indicates higher density. The central transverse sections of the 3DRs highlight internal structural differences, particularly emphasizing the density attributable to the genomic RNA in full RHDV virions., Peer reviewed

4 files, Dataset for ESSD submitted paper: Two decades of pHT measurements along the GO-SHIP A25 section, Agencia Estatal de Investigación, FICARAM+, PID2023-148924OB-I00 Agencia Estatal de Investigación, Ayudas para contratos predoctorales para la formación de doctores, PRE2020‐093138 Agencia Estatal de Investigación, PTA2022-021307-I European Commission, Euro GO-SHIP - Euro GO-SHIP: developing a Research Infrastructure concept to support European hydrography 101094690, Peer reviewed

Supplementary Table S1. Oligonucleotide primers used for RT-qPCR. Supplementary Table S2. Oligonucleotide primers used for dsRNA synthesis., This work was supported by the Spanish Ministry of Science, Innovation and Universities (MICIU/AEI/10.13039/501100011033) and the European Regional Development Fund (ERDF) ‘A way of making Europe’ (European Union), grant no. PID2019-103868RB-I00 (to J.C.), and by the Agency for Management of University and Research Grants (Government of Catalonia) grant 2021 SGR 00068 (to J.C.). A.R.-A. was the recipient of a predoctoral contract from the Spanish MICIU (PRE2020-092214). R.N.F. was supported by the University of Bergen (Norway). The authors also acknowledge the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000928-S) funded by the Spanish Agencia Estatal de Investigación (AEI) 10.13039/501100011033, Peer reviewed

Current vaccines for canine leishmaniosis (CanL) provide limited protection, underscoring the need for improved immunization strategies. Multi-epitope peptide vaccine delivered via nanoparticle systems represents a promising alternative but remains underexplored in canine clinical trials. Here, we report the results of a double-blind clinical trial (499/ECV) evaluating the safety and immunogenicity of HisDTC, a peptide vaccine targeting Leishmania infantum, encapsulated in poly(lactic-co-glycolic acid) PLGA nanoparticles and adjuvanted with Toll-like receptor 2 (TLR2) and TLR3 ligands. Forty healthy dogs were immunized with different vaccine formulations and monitored over 12 months. Immune responses were assessed by flow cytometry, ELISA, and in vitro macrophage infection assays, while safety was evaluated through clinical follow-up. Vaccination with HisDTC elicited a protective cellular response, including sustained IFN-γ production by CD4+ and CD8+ T cells, an IgG2a-skewed humoral response, and expansion of CD4+CD8α+ double-positive memory T cells. Notably, only HisDTC-vaccinated dogs exhibited a >80% reduction in in vitro macrophage infection, with protective effects persisting for up to 8 months post-immunization. Importantly, the formulation was well tolerated, with no adverse effects reported. These findings demonstrate that HisDTC delivered via PLGA nanoparticles induces durable, protective immunity against L. infantum in dogs and supports its further evaluation under natural exposure conditions., This research was supported by the grants PID2019-106487RB-I00 and PID2023-146579OB-I00 funded by Ministerio de Ciencia, Innovación y Universidades/Agencia Estatal de Investigación/AEI/10.13039/501100011033., Peer reviewed

17 pages, Appendix 1- Study titles, speakers, presentation in session 10 and participation in the final activity of session 10. Resilience and resilience-related concepts are highlited in the titles. Appendix 2- Case study descriptions. Appendix 3- Transcripts from the participants' answers during the group activity promoted by the MSEAS Session 10 conveners Appendix 4- Table with case studies features related with resilience-related concepts. Appendix 5 – Literature search strategy and screening results for resilience, adaptive capacity, vulnerability, and tipping points, represented in a flow diagram, I.M. was supported by the Portuguese Foundation for Science and Technology through FCT-IP Program Stimulus of Scientific Employment (https://doi.org/10.54499/2021.03721.CEECIND/CP1665/CT0005), CIIMAR’s strategic funding UIDB/04423/2020 and UIDP/04423/2020, and the project SHIFT-MARES (CEXC/5710/2024) funded by PLANAPP(Portugal). J.L. was funded by the project SEAwise (Horizon 2020; 101000318). S.K. was funded through the NOAA Climate Program Office, Climate and Fisheries Adaptation Program, and through the Research for Sustainability (FONA) program of the German Federal Ministry of Education and Research (BMBF). M.L. was supported by a PhD-fellowship (FPU program) and the project BITER (PID2020-114732RB-C31) of the Spanish Ministry of Science, Innovation and Universities. E.Q. and B.B. were supported by The German Federal Ministry of Education and Science as part of the SeaUseTip Project [grant no. 01LC1825C, 2018]. L.E. acknowledges the contribution to the European Union’s Horizon 2020 research and innovation programmes under grant agreement no. 869300 (FutureMARES project) and No 101059877 (GES4SEAS), as well as the support from the Catalan government through the iMARES research group of quality from the Institute of Marine Sciences (ICM-CSIC) and the “Severo Ochoa Centre of Excellence” accreditation to ICM-CSIC (#CEX2019-000928-S). L.E. and M.L. attendance to the MSEAS conference was also financially supported by ICES and PICES organizations. J.S.-C. was supported by JST SPRING (grant no. JPMJSP2106) and CONAHCYT (CVU 1108919). T.T.-O. was supported by the Marine Stewardship Council’s Ocean Stewardship Fund for Student Research Grant funding and Consejo Nacional de Humanidades, Ciencias y Tecnologías (CVU 775707), Peer reviewed

To the Spanish Ministry of Science and Innovation (MCI) through Agencia Estatal de Investigacion (AEI) and Fondo Europeo de Desarrollo Regional (FEDER, MCIN/AEI/10.13039/501100011033/FEDER UE) grant PID2023-146286OB-I00 “HOLOSEX” granted to LR. Institutional support through the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2024-001494-S) funded by AEI 10.13039/501100011033. Institutional support to CNAG was provided by the Spanish Ministry of Science and Innovation through the Instituto de Salud Carlos III, and by the Generalitat de Catalunya through the Departament de Salut and the Departament de Recerca i Universitats, Peer reviewed

Peer reviewed

This repository includes: - A comma-separated values file (eamo-bbmo_env-diversity.csv) containing the contextual and environmental data from the EAMO–BBMO time-series. This file contains information such as date, geographic position, season, and a selection of biological, physico-chemical and climatic parameters, as well as the computed alpha-diversity metrics. - A README file (eamo-bbmo_README.csv) explaining the meaning and units of each column name in the environmental dataset, This repository contains contextual and environmental data associated with samples from two coastal microbial time-series observatories located at contrasting latitudes: the Equatorial Atlantic Microbial Observatory (EAMO, 6°S) and the Blanes Bay Microbial Observatory (BBMO, 41°N, Mediterranean Sea). Sampling took place monthly from April 2013 to August 2016, with surface seawater measurements collected alongside eDNA metabarcoding samples, The EAMO data were generated with funds from FAPESP (process #2014/13139-3), CNPq (process 474759/2013-0), and the Brazilian Science Without Borders Program (BJT 013/2012; PVE 400313-2014-6). The BBMO data were obtained thanks to project DEVOTES (FP7-ENV-2012-308392), MINIME (PID2019-105775RB-I00), and MAORI (PID2022-136281NB-I00) as well as many others that have maintained the observatory, This work contributes to the Institut de Ciències del Mar "Severo Ochoa Centre of Excellence" accreditation CEX2024-001494-S funded by AEI 10.13039/501100011033 of the Spanish Ministry of Science and Innovation, Peer reviewed

Global warming is profoundly reshaping biodiversity. Until now, most research has focused on the impacts of extreme temperature events. However, in many ecosystems, it is becoming increasingly apparent that climate change is accelerating the onset of spring warming conditions. These advanced warming conditions can significantly disrupt critical biological processes such as reproduction, which is key for population persistence. While interest in phenological shifts has increased in recent years, their effects on marine foundation species, such as corals, remain poorly understood. Here, we combined observational and experimental approaches to assess the effects of advanced spring warming conditions driven by climate change on the reproduction of the Mediterranean octocoral Paramuricea clavata, a foundation species. Our findings reveal that a 2°C warming leads to a two-week advancement in P. clavata spawning, as evidenced by both field observations, and ex-situ experiments. These results underscore the role of advanced spring warming as a significant driver of phenological shifts in coastal marine ecosystems. Furthermore, we show that this phenological shift lead to a reduction in the number of spawning events, as well as decreases in larval biomass, survival rates, and settlement success. These findings highlight the urgent necessity to monitor phenological changes in foundational marine species, as such shifts can undermine the long-term viability of coral populations and contribute to substantial decline in associated biodiversity. Consequently, the increased vulnerability of species caused by phenological responses driven by seasonal changes may lead to more dramatic consequences of ocean warming than previously anticipated, This work contributes to the Institut de Ciències del Mar "Severo Ochoa Centre of Excellence" accreditation CEX2024-001494-S funded by AEI 10.13039/501100011033 of the Spanish Ministry of Science and Innovation, Peer reviewed