Resultados de investigación

The Type VI secretion system (T6SS) is a bacterial nanoweapon that injects toxic effectors into prokaryotic and eukaryotic cells. It is widely found among gram-negative bacteria and provides a significant fitness advantage in interbacterial competition. Pseudomonas putida KT2440 possesses three T6SS clusters (K1-, K2- and K3-T6SS) that combat phytopathogens. This makes this strain a potent biocontrol agent that protects plants from pathogens and can be further enhanced by a better understanding of its T6SS regulation. Although the core components of T6SS are conserved, the elements controlling its regulation differ among bacterial species. T6SS activity is regulated by various factors acting at different levels, from transcription to post-translational modification, to ensure precise control of its activity. Here, we demonstrate the critical importance that the three Rsm proteins, RsmIEA, have in controlling the K1-T6SS structural components and related orphan elements at the post-transcriptional level in Pseudomonas putida. We identified multiple Rsm-binding sites responsible for directly repressing the translation of T6SS proteins (Hcp1 and Hcp5) and their associated effectors (Tke2 and Tke7). Derepression of K1-T6SS mRNA in the rsmIEA mutant led to enhanced translation and expression of the K1-T6SS components and effectors, and critically increased the number of cells in the population with assembled T6SS. This results in a greater capacity to secrete toxins and kill prey cells via the T6SS-dependent mechanism. Finally, we demonstrate the K1-T6SS ability to kill environmental pathogens, including Salmonella enterica and Erwinia amylovora.

This technical data sheet contains all the data taken into account to solve the optimisation model for the case studies analysed in: Moreno, Silvia; Aragón-García, Alejandro; Villanueva-Perales, Ángel L.; Alonso Farinas, Bernabé; and F.D. Monaghan, Rory and Haro, Pedro, Multi-Objective Decision-Support Framework for Low-Carbon Hydrogen Supply Chains under Economic, Environmental, and Regulatory Constraints (1 February 2026). Available at SSRN: https://ssrn.com/abstract=6163626 or http://dx.doi.org/10.2139/ssrn.6163626. The data included relates to the all included scenario; the other scenarios are derived from and represent simplifications of the all included scenario, and the remaining scenarios can therefore be derived from this data sheet. For each sheet corresponding to each of the regions considered in the case studies, the data included covers biomass availability and transport, as well as data for each technology on conversion rates, auxiliary consumption, costs, environmental impacts (CO₂eq emissions), and plant size limits.

Los datos recopilan los registros de diferentes contaminantes a lo largo del experimento

En este dataset se recogen los modelos 3D de células de cáncer de mama de la línea MDA-MB-231 embebidas en hidrogeles de colágeno de diferente composición y concentración. Estos modelos 3D se crearon mediante el software Paraview, a partir de imágenes tomadas en un microscopio confocal (Leica Stellaris 8) en un stack de Z., Se incluyen archivos de paraview de una célula en cada tipo de hidrogel (colágeno bovino, de rata o mezcla de ambos) y cada concentración (0.8, 1.5 y 2.3 mg/ml).

Dataset generated in the work “Integrating remote sensing and ion balance to predict yield losses under saline irrigation in rice” whose main objectives were to (i) quantify the effects of increasing irrigation water salinity on rice plant growth, ion balance and yield; (ii) compare the salt-induced responses of indica and japonica subspecies; and (iii) assess the potential of remote sensing for monitoring salinity- induced stress and identifying critical phenological periods of sensitivity., The dataset comprises two csv files, ‘dataset_measurements.csv’ and ‘smoothed_indices.csv’. The former includes the following variables: day of year, farm_ID, water pH, water EC, subspecie, cultivar, BBCH, seeding DOY, harvest DOY, plant height, number of tillers, yield, leaf Na concentration, leaf K concentration, Na/K ratio. The second csv file includes the following variables: days after seeding, farm_ID, index value, smoothed index values, and index (a list of the vegetation índices analyzed in the manuscript).

El conjunto de dataset recoge una serie de informes generados a partir de un prompt creado por IA desde una descripción funcional realizada en lenguaje natural para la generación de un listado estructurado de recursos documentales periodísticos para la elaboración de todo tipo de productos periodísticos., PROMPT FINAL ASISTENTE DE DOCUMENTACIÓN PARA PERIODISTAS Informe prompt ChatGPT gratis Informe prompt ChatGPT PLUS Informe ChatGPT Plus consulta simple Informe Perplexity Informe Gemini

In this research, we investigated how chronic exposure to methylphenidate (MPH) during adolescence affects the maturation of the medial prefrontal cortex (mPFC), with a specific focus on parvalbumin-expressing (PV+) interneurons and long-term attentional performance. Wistar rats of both sexes received therapeutically relevant doses of MPH during distinct adolescent developmental windows and were later assessed in adulthood using a sustained attention task (SAT) to evaluate potential persistent cognitive effects. This dataset contains behavioral performance measures from the SAT (hit rate, correct rejection rate, omissions, reaction time, and performance under varying stimulus durations), along with quantified counts of PV+ interneurons in prelimbic and infralimbic mPFC regions. Additional variables include sex, treatment dose, developmental exposure window, age at treatment onset, and control groups. The dataset enables analysis of how developmental MPH exposure influences GABAergic maturation and adult attentional function.

1. Excel con el registro de aislados bacterianos y metadata

El archivo MS Excel contiene datos de superficie, producción y rendimiento del centeno de 34 países durante el periodo 1870-2024., El archivo MS Excel contiene datos de superficie, producción y rendimiento del centeno de 34 países durante el periodo 1870-2024 (tablas S1, S2 y S3, respectivamente). Las tablas S4, S5 y S6 suponen un resumen de las anteriores por décadas. La siguiente tabla muestra los datos tomados para el análisis lineal , mientras que la siguiente muestra los datos de comparación entre Rusia y el mundo.

El objetivo de este estudio fue evaluar la eficacia en el control de la fusariosis vascular del tomate de dos agentes de control biológico en varios sustratos con distinto nivel de supresividad. Estos sustratos fueron compost de orujo de vid, compost de corcho, y fibra de coco. Los agentes de control biológico eran un aislado perteneciente a la especie Fusarium solani y otro perteneciente al género Streptomyces. Los resultados tras la inoculación con Fusarium oxysporum f.sp. lycopersici mostraron mayor producción de tomate en fibra de coco cuando se inoculaban con estos agentes de control biológico. En compost de orujo de vid (sustrato con alta supresividad) no se detectó mejora con la aplicación de los agentes de control biológico. En el compost de corcho, que tiene una supresividad moderada A19 llevó la supresividad al nivel del compost de orujo de vid. Luego la naturaleza del compost y el agente de control biológico aplicado influyen en la respuesta a la fusariosis vascular del tomate.