Resultados de investigación

METHODOLOGICAL INFORMATION AND SAMPLE SIZE This study followed a longitudinal, prospective, and multi-cohort quasi-experimental design. The initial clinical recruitment comprised 72 breast cancer survivors referred by the Murcian Health Service (Servicio Murciano de Salud, SMS) across health zones 1, 6, and 7. The source population presented high clinical heterogeneity, incorporating patients undergoing both conservative surgical procedures (lumpectomy, quadrantectomy, oncoplasty; 37.5%, n = 16) and radical/non-conservative interventions (complete, modified, or bilateral mastectomy; 52.8%, $n = 23$; along with additional bilateral procedures, 9.7%, $n = 4$), combined with or without axillary lymph node dissection and sentinel lymph node biopsy. The final statistical monitoring focused on a strict per-protocol sample of 36 participants ($n = 36$) who complied with the clinical compliance threshold (Cumulative Attendance Percentage > 33.33%) and possessed complete data rows across all operational time points. Neuromuscular performance was tracked at baseline pre-intervention (PRE) and following a 12-week supervised resistance training macrocycle (POST). Because each participant was measured repeatedly across both upper limbs (Operated vs. Non-Operated), the final longitudinal data matrix contains a total volume of exactly $N = 128$ independent longitudinal observations ($obs$) clustered hierarchically within patients.

Introduction: Research on physical activity and breast cancer has grown steadily over the past 25 years, with a progressive shift from physiological and safety-related questions towards clinical, psychosocial and implementation domains. Objective: To describe research trends, key actors and thematic foci in the scientific literature on physical activity and breast cancer between 2000 and 2025. Methods: We included articles and reviews without language restrictions, published between 2000 and 2025 and indexed in Scopus, PubMed and Web of Science. Records were exported in CSV format and analysed using the Bibliometrix package in R. We described publication and citation volume, collaboration patterns (countries, institutions and authors), core journals according to Bradford’s law, and the semantic evolution of the field based on keyword co-occurrence and trend topics. Results: We identified 13,329 documents on physical activity and breast cancer, with a sustained increase in both publications and citations over the study period. A marked geographical concentration was observed, with leadership by high-income, mainly Anglophone countries and a limited number of specialised editorial cores, as well as a small group of highly influential authors. The thematic analysis showed a shift from studies focused on functional capacity and safety towards clinical practice guidelines, survivorship and quality of life, alongside emerging lines such as prehabilitation, remote interventions and tele-exercise. Conclusions: The field of physical activity and breast cancer shows a high and growing scientific output with a clear clinical orientation. Integrating exercise prescription into oncological care pathways, defining and standardising dose–response schemes according to tumour phenotype and clinical context, and expanding multicentre studies that include under-represented regions and populations—particularly in low- and middle-income countries—are recommended priorities for future research and practice.

Búsqueda en Pubmed, Scopus, WOS y Eric de competencias en IA.

Isolation of pericytes from mice adipose tissue. Cell culture and knock-down of Lamp2a gene via lentiviral transduction. Coculture of pericytes and glioblastoma cell lines and analysis by RT-qPCR, western-blot and flow cytometry. Generation of glioblastoma in vivo model in mice and intravenous treatment with Lamp2a knoch down pericytes. Analysis of tumor area with immunofluorescent stainings of brain sections.

Datos utilizados para la confección de "AntimiR-199a treatment protects against cardiac hypertrophy and fibrosis following myocardial infarction", como parte del trabajo completo "The miRNA199a/SIRT1/P300/Yy1/sST2 signaling axis regulates adverse cardiac remodeling following MI", con DOI https://doi.org/10.1038/s41598-021-82745-9