Resultados de investigación

Detected variants passing all filters categorized as pathogenic or likely pathogenic somatic mutations., Instituto de Salud Carlos III Cancer Research UK Fundación Científica Asociación Española Contra el Cáncer Fondazione Italiana per la Ricerca sul Cancro Fondazione Regionale per la Ricerca Biomedica International Myeloma Foundation European Research Council European Commission Multiple Myeloma Research Foundation, Peer reviewed

Quality control of the 555 NGS samples included in the study., Instituto de Salud Carlos III Cancer Research UK Fundación Científica Asociación Española Contra el Cáncer Fondazione Italiana per la Ricerca sul Cancro Fondazione Regionale per la Ricerca Biomedica International Myeloma Foundation European Research Council European Commission Multiple Myeloma Research Foundation, Peer reviewed

Instituto de Salud Carlos III Cancer Research UK Fundación Científica Asociación Española Contra el Cáncer Fondazione Italiana per la Ricerca sul Cancro Fondazione Regionale per la Ricerca Biomedica International Myeloma Foundation European Research Council European Commission Multiple Myeloma Research Foundation, Peer reviewed

Clinical and tumor microenvironmental characteristics of untreated, newly diagnosed active MM patients having normal (n = 129) vs mutant HPC (n = 38). P-value is calculated using corrected χ2 for dichotomous variables, and Kruskal-Wallis for numerical variables., Instituto de Salud Carlos III Cancer Research UK Fundación Científica Asociación Española Contra el Cáncer Fondazione Italiana per la Ricerca sul Cancro Fondazione Regionale per la Ricerca Biomedica International Myeloma Foundation European Research Council European Commission Multiple Myeloma Research Foundation, Peer reviewed

Hematologic toxicity during treatment observed in patients enrolled in the GEM2012MENOS65, GEM-CLARIDEX and GEM-CESAR clinical trials compared to mutant HPC detected at baseline. P-value is calculated using corrected χ2 test., Instituto de Salud Carlos III Cancer Research UK Fundación Científica Asociación Española Contra el Cáncer Fondazione Italiana per la Ricerca sul Cancro Fondazione Regionale per la Ricerca Biomedica International Myeloma Foundation European Research Council European Commission Multiple Myeloma Research Foundation, Peer reviewed

Clinical characteristics of MM patients who develop SPM (n = 29) vs those who have not (n = 279). P-value is calculated using corrected χ2 for dichotomous variables, and Kruskal-Wallis for numerical variables., Instituto de Salud Carlos III Cancer Research UK Fundación Científica Asociación Española Contra el Cáncer Fondazione Italiana per la Ricerca sul Cancro Fondazione Regionale per la Ricerca Biomedica International Myeloma Foundation European Research Council European Commission Multiple Myeloma Research Foundation, Peer reviewed

Mycoplasma motility in the presence of antibodies., In embryos, epithelial sealing proceeds with progressive zipping eventually leading to a scar-free epithelium and ensuring the assembly of body segments in insects and neural tube in mammals. How zipping is mechanically controlled to promote tissue fusion on long distances, remains unclear. Combining physical modeling with genetic and mechanical perturbations, we reveal the existence of a transient contractile seam that generates forces to reduce the zipping angle by force balance, consequently promoting epidermal sealing during Drosophila embryogenesis. The seam is formed by the adhesion of two tissues, the epidermis and amnioserosa, and is stabilized by the tensions generated by the segment boundaries. Once a segment is zipped, the seam disassembles concurrently with the inactivation of the Jun kinase pathway. Thus, we show that epithelial sealing is promoted by a transient actomyosin contractile seam allowing sequential segment assembly., Peer reviewed

An active Nap complex cycles between “open” and “closed” states, with the binding site to sialic oligosaccharides becoming alternatively accessible and inaccessible for binding. As shown in this work, during the nap cycle, structural rearrangements are experienced mainly by the C-domain of P1 (brown), with hinge rotations of about 175°, while the ectodomains of P40/P90 (pink) remain mostly unchanged. The wide movement of the C-domain from P1 drags, by contiguity, transmembrane helices thus reaching the internal parts of the TO (represented here simply as a line of elastic (red) and rigid (blue) components with possible displacements shown as black arrows). Binding of antibody P1/MCA (green) to the C-domain of P1 halts the cycle, trapping the “closed” conformation of the Nap. The structures known prior to this work are indicated using soft colors., Peer reviewed

a) Transmembrane helices of adhesins from the M. pneumoniae cluster of mycoplasmas contain three highly conserved Engelman motifs (GxxxG sequences), referred as E1 (P140) and E2, E3 (P110) in M. genitalium. b) Mutational analysis, performed in the Engelman motifs of P140 and P110, indicate that motifs E2 and E3 from P110 have important and synergic contributions to adhesion. On the contrary, no effects were observed when E1 from P140 was mutated. c) Changes in adhesion correlate with increasing rates of cells presenting multiple TOs phenotypes., Peer reviewed

a) Ribbon representations, with three 90° apart views, of the complex between the ectodomain of P1 (brown) and the Fab fragment of Mab P1/MCA4 (light and heavy chains in blue and green, respectively). Insets indicate the main interactions between P1 and the Fab. The epitope is located in the C-domain of P1 closed to the mycoplasma membrane, which is also displayed as a reference. b) Two views of the Fab(P1/MCA4) (shown in green) and the extracellular region of the Nap, with P1 (brown) and P40/P90 (pink transparent). In the “open” conformation of the Nap (left panel) the P1/MCA4 epitope, buried at the center of the Nap, is totally inaccessible to antibodies and a Fab placed there would catastrophically clash with the adhesins. Therefore, to expose the P1/MCA4 epitope the C-domain of P1 has to experience an important rearrangement (right panel)., Peer reviewed