Resultados de investigación

Background. Albuminuria is recognized as an early marker of kidney injury and an independent predictor of cardiovascular events. Yet the frequency of abnormal albuminuria (AA; UACR ≥ 30 mg/g) and mildly increased albuminuria (MIA; 10–29 mg/g) in Mediterranean populations is still poorly characterized. Methods. A cross-sectional, multicentre study including all urine albumin-to-creatinine ratio (UACR) and estimated glomerular filtration rate (eGFR) records from July 1, 2021, to June 30, 2023, in individuals ≥ 45 years (23 860 adults [median age 64 years; 54 % women]) within a Health Management Area was done. We used KDIGO 2024 classification, adding a new sub-category for MIA. Prevalence with 95 % confidence intervals (CI) was calculated and stratified by sex, age, diabetes, and hypertension. Findings. The overall prevalence of AA was 9.2 % (95 % CI 8.8–9.6), higher in men (10.8 %) than in women (7.9 %) and rose steadily with age: 3.9 % (45–59 y), 8.5 % (60–74 y), and 18.1 % (≥ 75 y). AA reached 18.8 % among those with diabetes and 22.7 % in diabetes plus hypertension, versus 3.8 % in only hypertensive people, and 1.5 % without both conditions. An inverse relationship with kidney function was observed (4.0 % in stage G1 versus 55.2 % in G4–G5). MIA was present in 9.9 % (95 % CI 9.5–10.3), showing similar distribution patterns at lower magnitudes; a peak of 16.2 % occurred at eGFR 60–74 mL/ min/1.73 m², falling to 13.1 % in G4–G5. Interpretation. Abnormal and mildly increased albuminuria affect nearly one in five adults aged 45 and over in Mediterranean primary care. Given its strong association with age, diabetes, hypertension, and declining kidney function, systematic UACR screening- including for MIA- must be prioritized to enable early detection and intervention., Yes

Data was acquired for the study published in the article titled: Anti-obesogenic effect of lupin-derived protein hydrolysate through modulation of adiposopathy, insulin resistance and gut dysbiosis in a diet-induced obese mouse. This study examines the effects of a lupin protein hydrolysate (LPH) on obesity, adipose tissue dysfunction and gut dysbiosis in obese mice. LPH was characterized and protein concentration, ashes, moisture, fiber, fats, soluble sugars and phenol content were measured as described. Amino acid composition was determined using standard amino acid mix solution. The molecular weight profile was obtained by molecular exclusion chromatography. Eight-week-old male C57BL6/N mice were housed under standard conditions and mice were randomly assigned to three groups: mice fed a standard diet (SD), a high-fat diet (HFD) or an HFD and treated intragastrically with LPH (HFD+LPH) at a dose of 100mg/kg five days a week for 12 weeks. The SD and HFD groups were treated with vehicle under the same conditions as the HFD+LPH group. Food consumption and individual body weight were measured weekly. Animals were sacrificed with an intraperitoneal injection of sodium thiopental and blood was collected by cardiac puncture. Epidydimal adipose tissue (EpiWAT) was dissected, snap-frozen and stored at -80ºC until use or fixed in a 4% paraformaldehyde (PFA) solution. Stool was collected prior to euthanasia and stored at -80ºC. Serum biochemical profile was measured by chemoluminiscence in the Cobas Integra 400 (Roche Diagnostics, Indianapolis, IN, USA) at the Estación Biológica de Doñana (EBD-CSIC, Seville, Spain). Serum concentrations of leptin, adiponectin and insulin were quantified using enzyme-linked immunosorbent assays (ELISA) kits. Adipose tissue samples fixed in 4% PFA were dehydrated, embedded in paraffin blocks and sliced 4μm thick, stained with hematoxylin-eosin and cover mounted for posterior observation. Photomicrographs were obtained using Leica THUNDER microscope and Leica Application Suite X software. Adipocyte morphology and number of crown-like structures was blinded analyzed using ImageJ v1.53h public software. High throughput 16S rRNA gene amplicon analysis was performed using total DNA extracted from stool. 16S rRNA V3–V4 region was sequenced using MiSeq protocol and instruments. The raw sequences were processed using mothur., Dataset entitled “hydrolysate_composition.xlsx” includes macromolecular and aminoacidic composition (first sheet) and chromatogram information (second sheet) of LPH obtained from three independent measurements. Dataset entitled “obesity_markers.xlsx” includes experimental group and numerical data (columns) obtained from the measurement of parameters from animals (rows) used in the present study.

Background Antibiotics could modify multidrug-resistant microorganisms (MDRO) populations in urban wastewater. Water treatment plays a major role in their control. This study aimed to explain the characteristics of multidrug-resistant Gram-negative bacteria in the wastewater of a city in southern Spain, the reduction achieved by treatment and the influence of human antibiotic prescriptions. Methods We collected 24-hour composite water samples from four wastewater treatment plants (WWTPs) over one year (2021-2022) and cultured them quantitatively. All acquired ESBL/carbapenemase isolates were sequenced and genomes were compared with those of clinical isolates. Linear mixed model regression analyses were performed to assess the WWTP effect and the association with human antibiotic prescriptions. Findings Aeromonas predominated among the MDROs and KPC-2 was the main carbapenemase detected in the influents. The 44% and 30% of E. coli and K. pneumoniae influent isolates, respectively, belonged to high-risk clones; 10·6% of Enterobacteriaceae could be matched to clinical isolates and a strain from a current hospital outbreak was found in raw samples. WWTPs reduced ESBL and carbapenemase producers by 1·4-log and 1·8-log, respectively. New MDROs and persistence of certain strains were detected in effluents. Quinolone and cephalosporin prescriptions, flow-rate and population density were associated with higher OXA-48 producer counts. Interpretation Despite the reduction, additional technologies should be implemented in WWTPs receiving hospital discharges. Due to the predominance of environmental species, culture and metagenomic approaches should be combined to differentiate human and sewages origin for antibiotic resistance monitoring. Antibiotic stewardship could be a strategy to reduce the release of MDROs., 1. Excel con el registro de aislados bacterianos y metadata 2. Word con los cálculos estadísticos

The dataset includes the raw data from the clinical score and flow cytometry analyzes carried out in the work titled " Melatonin synergistically potentiates the effect of methylprednisolone on reducing neuroinflammation in the experimental autoimmune encephalomyelitis mouse model of multiple sclerosis". This study shows the protective synergistic effect of co-treatment with melatonin and methylprednisolone on reducing the severity of EAE by decreasing CD4+ lymphocytes, B cells, macrophages and dendritic cells in the CNS, as well as modulating the population of infiltrated T and B cells toward regulatory phenotypes to the detriment of pro-inflammatory effector functions. In addition, treatment with melatonin from the clinical onset of EAE improves the natural course of the EAE and the response to a subsequent treatment with methylprednisolone in a later relapse of the disease. Eight-week-old female C57BL/6N mice were immunized immunization with 100 μg of MOG35–55 (Cambridge Research Biochemicals, Cleveland) emulsified in CFA (Sigma) containing 50 μg of heat-killed Mycobacterium tuberculosis (H37Ra, ATCC 25177) by subcutaneous injection in both hind legs and two doses of intraperitoneal pertussis toxin (200 ng/day) (List Labs, California) on days 0 and 2 post-induction. Animals were randomly divided to receive melatonin at a concentration of 80 mg/kg and/or methylprednisolone at a concentration of 40 or 160 mg/kg in different treatment regimens. Mice were sacrificed at the peak of the disease (day 15 after induction) and after perfusion, the CNS was collected, homogenized and enzymatically dissociated with 1.87 mg/ml of collagenase IV (Worthington) and 0.25 mg/ml of DNase I (AppliChem) for 35 min at 37°C to obtain a suspension of single cells. Subsequently, a 37%:70% discontinous percoll gradient was carried out to isolate CNS-infiltrating mononuclear cells. To assess the profile of infiltrated immune cells in the CNS, cells were stained for the following antibodies against surface markers: CD45, CD4, CD8α, CD19, CD11b, CD11c, CD44, CD62L, B220, CD138, PD-1 (CD279), CTLA-4 (CD152), FAS (CD95), and CD25. To identify Treg and analyze intracellular production of TNF, IFN-γ and IL-10, after surface staining, cells were fixed and permeabilized using the The dataset includes the raw data from the clinical score and flow cytometry analyzes carried out in the work titled " Melatonin synergistically potentiates the effect of methylprednisolone on reducing neuroinflammation in the experimental autoimmune encephalomyelitis mouse model of multiple sclerosis". This study shows the protective synergistic effect of co-treatment with melatonin and methylprednisolone on reducing the severity of EAE by decreasing CD4+ lymphocytes, B cells, macrophages and dendritic cells in the CNS, as well as modulating the population of infiltrated T and B cells toward regulatory phenotypes to the detriment of pro-inflammatory effector functions. In addition, treatment with melatonin from the clinical onset of EAE improves the natural course of the EAE and the response to a subsequent treatment with methylprednisolone in a later relapse of the disease. Eight-week-old female C57BL/6N mice were immunized immunization with 100 μg of MOG35–55 (Cambridge Research Biochemicals, Cleveland) emulsified in CFA (Sigma) containing 50 μg of heat-killed Mycobacterium tuberculosis (H37Ra, ATCC 25177) by subcutaneous injection in both hind legs and two doses of intraperitoneal pertussis toxin (200 ng/day) (List Labs, California) on days 0 and 2 post-induction. Animals were randomly divided to receive melatonin at a concentration of 80 mg/kg and/or methylprednisolone at a concentration of 40 or 160 mg/kg in different treatment regimens. Mice were sacrificed at the peak of the disease (day 15 after induction) and after perfusion, the CNS was collected, homogenized and enzymatically dissociated with 1.87 mg/ml of collagenase IV (Worthington) and 0.25 mg/ml of DNase I (AppliChem) for 35 min at 37°C to obtain a suspension of single cells. Subsequently, a 37%:70% discontinous percoll gradient was carried out to isolate CNS-infiltrating mononuclear cells. To assess the profile of infiltrated immune cells in the CNS, cells were stained for the following antibodies against surface markers: CD45, CD4, CD8α, CD19, CD11b, CD11c, CD44, CD62L, B220, CD138, PD-1 (CD279), CTLA-4 (CD152), FAS (CD95), and CD25. To identify Treg and analyze intracellular production of TNF, IFN-γ and IL-10, after surface staining, cells were fixed and permeabilized using the FoxP3/transcription factor staining buffer set (eBioscience) and stained with anti-FoxP3 or anti-TNF, -IFN-γ and -IL-10, respectively. The intracellular production of cytokines was carried out in cells cultured at 2.5 × 106 cells/ml with RPMI 1640 supplemented with 5% fetal bovine serum, 1% L-glutamine and 1% of penicillin/streptomycin incubated in the presence or absence of Phorbol-Myristate-Acetate and Ionomycin (PMAI, Sigma) with brefeldin A (eBioscience) for 5 h. Dead cells were excluded from analyses using the LIVE/DEAD® Fixable Dead Cell Stain Kit (Invitrogen). FACS analysis was performed using a Cytek Aurora spectral cytometer, and data were analyzed using FlowJo software (Treestar)., The data set titled “Mel+MPD co-therapy.xlsx” includes numerical data from flow cytometry analysis (first sheet) and clinical score analysis (second sheet). A legend is also incorporated into the third sheet detailing the different experimental groups of the study.

The dataset includes the raw data from the weight, clinical score, and number of sequences carried out in the work titled "A lupin (Lupinus angustifolius) protein hydrolysate decreases the severity of experimental autoimmune encephalomyelitis, a preclinical model of multiple sclerosis". LPH reduced the clinical score of the mice compared to the control group, as well as the maximum and cumulative scores, without changing the day of onset of the symptoms while the therapeutic intervention did not significantly improve the severity of the disease. Fifty-six female C57BL/6N mice (8-weeks-old) from the University of Seville Animal Facility were housed under standard conditions with ad libitum access to water and food. For the induction of EAE, 100 µg of MOG35–55 peptide (Cambridge Research Biochemicals, Cambridge, UK) emulsified in complete Freud´s adjuvant (CFA) containing 4 mg/mL of heat-inactivated Mycobacterium tuberculosis (Sigma-Aldrich, St. Louis, MO, USA) was inoculated subcutaneously. Furthermore, two doses of 400 ng pertussis toxin (Enzo Life Sciences, Farmingdale, NY, USA) were administered intraperitoneally on days 0 and 2 post-induction (p.i.) to induce the disease. An EAE clinical sign score was assigned daily to each mouse as follows: 0 = no clin-ical sign; 1 = no tail tone; 2 = impaired righting reflex; 3 = paralysis of one hind limb; 4 = paralysis of both hind limbs; 5 = paralysis of both hind limbs and one forelimb; 6 = if the score was greater than 5 (including moribund or dead mouse). LPH sequencing was performed using nano high-performance liquid chromatography coupled to an Orbitrap Elite mass spectrometer. The BIOPEP-UWM database was used to identify the motifs with proven biological activity., The data set titled “LPH_EAE_Dataset.xlsx” includes numerical data from mice weight and clinical score, and the numbers of sequences with a determinate biological activity.

Reports related to the study published under the title "Unveiling protective mechanisms of wild olive (wild olive tree) oil in retinal pigment epithelial cells with hypertensive phenotype". This study was funded thanks to the project of the state plan PID2019-109002RB-I00 and the Junta de Andalucía ProyExcel_00516. In both projects, the beneficial properties of wild olive oil at the retinal level are described. Specifically, these data refer to the antioxidant properties of extracellular vesicles obtained from the plasma of animals treated with diets enriched in wild olive oil and their effect on retinal pigment epithelium cells subjected to a hypertensive phenotype. Dataset generated were collected between 2021 and 2024, part of which is the result of research stays abroad in collaboration with the Karolinska Institutet., Figura 1_ MTT and DCFHDA levels.pzfx: data corresponding to manuscript Figure 1 on cell viability and ROS. Figura 2_CAT.pzfx: data corresponding to Figure 2 in manuscript indicating protein and gene expression of CAT-1. Figura 2_Datos_vi.pzfx: data corresponding to Figure 2 in manuscript indicating L-arginine initial velocity. Figura 2_Transporte_articulo.pzfx: data corresponding to Figure 2 in manuscript indicating L-arginine transport and CAT-1 kinetic. Figura 3_DCFHDA_ATPCR.pzfx: data corresponding to Figura 3 of manuscript indicating DHFDA levels and WB and RT-PCR of angiotensin receptors. Figura 4_NADPH-MITOSOX.pzfx: data corresponding to Figure 4 of manuscript indicating activity and expression of NADPH oxidase and mitochondrial ROS. Figura 5_NO-Arginasas.pzfx: data corresponding to Figure 5 of manuscript indicating NO and ADMA values and protein and gene expression of arginases.

The dataset includes the raw data carried out in the work titled "Olive seed protein hydrolysates possess antioxidant and anti-inflammatory activities in mice and human cells". The olive seed protein hydrolysates (OSPHs) demonstrated antioxidant and immunomodulatory effects on RAW 246.7 cells and human peripheral blood mononuclear cells, suggesting their potential for use in developing new functional foods or nutraceuticals. The aim of this work was to deepen the study of multifunctional behavior of OSPHs obtained from the Frantoio cultivar of Olea europaea L., prepared using two food-grade enzymes, namely Alcalase® (AH) and Papain (PH), studying their antioxidant capacity and possible immunomodulatory activity on a murine monocyte/macrophage cell line (RAW 246.7) and on the human peripheric blood mononuclear cells (PBMCs)., The data set titled “OliveSeedProteinHydrolysates_Dataset.xlsx” includes raw data from in vitro experiments.

Audiovisual realitzat pel Departament de Física de la universitat de Girona. Idea: Joaquim Pérez Losada. Documental, guió i muntatge: Anna Bayó Duran. Agraïments: Imma Merino, Àngel Quintana i Marta Sureda, La materia que se observa es el Individuo, que a través de varios procesos -en la física serían temperatura y presión- acaba cohesionando en el concepto de masa. Una masa que, como se verá en el vídeo, tiene distintas formas. Lo que una vez fué singular, termina formando parte de lo común. Hay en todo el vídeo un elemento que funciona como coagulante y que, a la vez, sirve para vertebrar todo el discurso. Este es, ni más ni menos, que el documental 'Century of the Self' (Adam Curtis, 2002) (Adam Curtis, 2002) que se utiliza para mostrar como este control sirvió para unir o dividir a los sujetos al largo de la historia. Así, Century of the self saldrá como reflexión para presentar lo que los fragmentos de otras películas expresarán el individuo paso a ser un individuo completamente controlable y como el proyecto se estructura en dos partes. La primera, titulada El individuo y la perdida de libertad y que sitúa al espectador en un espacio donde el sujeto deja de ser libre y las implicaciones que este acto puede ocasionar al conjunto de la población. La segunda parte, La manipulación de la sociedad y las formas de la masa nos sitúa, a su vez, a las distintas formas y al poder que ha conseguido la masa en la contemporaneidad, 4571.mp4

És un projecte dels Serveis Limgüístics de set universitat catalanes: UAB, UB, UdG, UPC, UdL, UIC i Abat Oliba-CEU, Projecte que ha rebut un ajut Interlingua de la Generalitat de Catalunya, Coordinació: Enric Serra i Virgínia Castillo (Servei de Llengües de la UAB); Contingut: David Owen (Servei de Llengües de la UAB) i Kari Friedenson; Veu: Kari Friedenson i Producció audiovisual: Agustí Camps (Servei de Llengües de la UAB), Introduction to the specialised language: definition, skills, characteristics (formality, objectivity and neutrality, clarity and precision and universality), Introducció al llenguatge d'especialitat o específic: definició, manifestacions, i característiques bàsiques (grau de formalitat alt, objectivitat i neutralitat, claredat i precisió i universalitat), 5939.mp4

És un projecte dels Serveis Limgüístics de set universitat catalanes: UAB, UB, UdG, UPC, UdL, UIC i Abat Oliba-CEU, Projecte que ha rebut un ajut Interlingua de la Generalitat de Catalunya, Coordinació: Enric Serra i Virgínia Castillo (Servei de Llengües de la UAB); Contingut: David Owen (Servei de Llengües de la UAB) i Kari Friedenson; Veu: Kari Friedenson i Producció audiovisual: Agustí Camps (Servei de Llengües de la UAB), The different types and characteristics of specialized texts, Les diferents tipologies i característiques dels textos especialitzats, 5941.mp4