Resultados de investigación

S1. CATH-M and PYRO-M membranes synthesis and manipulation S2. FTIR analysis of the synthesized membranes S3. X-ray photoelectron spectroscopy curve-fitting of the synthesized membranes. Table S1. Elemental analysis. S4. Morphology of the membranes. S5. Long-term degradation test. S6. Morphological changes after degradation test. S7. Chemical changes after degradation test. S8. Antibacterial properties. S9. CATH-M membrane manipulability in a real environment. S10. Ex vivo adhesion on brain. S11. Mechanical and adhesion tests. S12. Glioblastoma cells cytotoxic screening. S13. Effect against different tumor cells lines and healthy cells. S14. CATH-H time course cytotoxicity. S15. Clonogenic test. S16. Local effect. S17. Comparison with standard-of-care treatments. Table S2. Inhibitors and inducers details. S18. CATH-H Caspase-independency cell death. S19. CATH-M and necroptosis inhibitor. S20. CATH-M and autophagy inhibitors. S21 CATH-M and parthanatos inhibitors. S22. CATH-M and macromolecular synthesis inhibitors. S23. CATH-M and ferroptosis inhibitors. S24. CATH-M and oxidative damage inhibitor. S25. LN229 morphology over the membranes. S26. LN229 cell death over the membranes. S27. Primary cortical astrocytes morphology over the membranes. S28. Primary cortical astrocytes viability over the membranes. S29. Spheroids early culture by scanning electron microscopy (SEM). S30. Spheroids early culture by optical microscopy. S31. Spheroids progression. S32. Inserts test scheme. S33. Influence in the LN229 cell migration. S34. Migrated and non-migrated cells visualization. S35. Membranes strong cell adhesion. S36. Alterations in the LN229 internal structures. S37. Seahorse test. S38. LN229 cell death under oxidative stress. S39. CATH-M reactive oxygen species (ROS) production properties. S40. Lipid peroxidation. S41. LN229-lysate protein microarray., Video S1. Synthesis of the CATH-M and PYRO-M membranes. Video S2. Manipulation of the CATH-M and PYRO-M membranes. Video S3. CATH-M insertion in a brain cavity. Application and removal of an 8 mm CATH-M disc in an ex vivo model (pig brain), demonstrating the manipulability and flexibility. Video S4. Representative bright field and Hoechst 33342 (H42) composition of the LN229 cells that remained in the CATH-M and PYRO-M membranes after being trypsinized. The cells are homogeneously distributed along the shape of the membranes. Video S5. Lipid peroxidation. 3D visualization of the lipid peroxidation results for the control, CATH-M and PYRO-M. Blue = nuclei, RED = dye reduced-state, Green= dye oxidised-state., Peer reviewed

MYC downregulation reverses the prometastatic phenotype induced by etomoxir and MCM, European Commission National Natural Science Foundation of China Associazione Italiana per la Ricerca sul Cancro Instituto de Salud Carlos III Worldwide Cancer Research Fundación Científica Asociación Española Contra el Cáncer Fundación Fero Cancer Council NSW (Australia) National Health and Medical Research Council (Australia) German Research Foundation Shanghai Municipal Education Commission Ministero della Salute, Peer reviewed

Effects of MYC inhibition with mycro3 on EMT genes expression and changes induced by PGC1A overexpression at the functional level, European Commission National Natural Science Foundation of China Associazione Italiana per la Ricerca sul Cancro Instituto de Salud Carlos III Worldwide Cancer Research Fundación Científica Asociación Española Contra el Cáncer Fundación Fero Cancer Council NSW (Australia) National Health and Medical Research Council (Australia) German Research Foundation Shanghai Municipal Education Commission Ministero della Salute, Peer reviewed

Table of contents: 1. Spectral characterization: 1.1 1H NMR of m1; 1.2 1H NMR of m2; 1.3 1H NMR and 13C NMR of b1; 1.4 1H NMR and 13C NMR of b2; 1.5 1H NMR of p1and p(1-4). -- 2. Synthesis and characterization of p1 and p(1-4): 2.1 Optimization of p1; 2.2 Optimization of p(1-4). -- 3. Mechanical characterization of the photocurable materials: 3.1 DMTA plots; 3.2 Real time FTIR conversion plots; 3.3 Summary of the photocurable formulations characterization. -- 4. Data of additional studies of p(b2-PEGDA700): 4.1 Burst Pressure tests, 4.2 Swelling study, 4.3 ESR determination, 4.4 Degradation study, Peer reviewed

Donana_N2O_CH4_marsh_2016-2018.csv provides data from the 16 field surveys conducted between March 2016 and March 2018 (99 water samples). Temperature, salinity and pH were measured in situ. Dissolved gases, inorganic nutrients, total dissolved nitrogen, dissolved organic carbon, dissolved oxygen and chlorophyll a were measured in the laboratory. DATETIME_UTC is the sampling date and time in Coordinated Universal Time (YYYY-MM-DD HH:MM, UTC). SITE is the site number (1-7 along the Guadalquivir salinity gradient; 9 = Marisma del Guadiamar). HYDRO_CLASS is the hydrological class of the site (creek_inflow = tidal creek; interior = sheet-flow marsh interior; guadiamar = Guadiamar endpoint). LAT and LON are the site latitude and longitude in decimal degrees (WGS84). TEMPERATURE_C and SALINITY are the in-situ water temperature (degrees Celsius) and salinity (practical salinity scale, dimensionless). PH is the in-situ pH on the NBS scale. O2_MG_L is dissolved oxygen by Winkler titration (mg L-1). AOU_UMOL_KG is the apparent oxygen utilisation (micro mol per kilogram; calculated, not measured). CHLOROPHYLL_A_UG_L is chlorophyll a (micro g L-1). DOC_MG_L is dissolved organic carbon and TDN_MG_L is total dissolved nitrogen, both in mg L-1. NH4_UMOL_L, NO2_UMOL_L and NO3_UMOL_L are ammonium, nitrite and nitrate concentrations, respectively, and PO4_UMOL_L and SIO4_UMOL_L are phosphate and silicate concentrations, all in micro mol L-1. PCO2_UATM is the partial pressure of CO2 (micro atm; calculated from the measured pH and total alkalinity, not measured directly). N2O_NMOL_L and CH4_NMOL_L are the dissolved nitrous oxide and dissolved methane concentrations (n mol L-1)., This data set comprises discrete water-column measurements of dissolved nitrous oxide (N2O) and methane (CH4), together with the accompanying physico-chemical and biogeochemical variables, used to assess the role of the inundated tidal salt marshes of Donana National Park (SW Spain; Guadalquivir estuary and Guadiamar tributary, bounding box approximately Long: -6.39 to -6.27, Lat: 36.86 to 37.08, Datum: WGS84) in estuarine nitrogen removal and in regulating nitrous oxide emissions. The data document the spatial gradient of dissolved gases and nutrients across the marshland, from the tidal creeks that convey estuarine water into the marsh to the inner sheet-flow areas and the upstream Guadiamar marsh, over the period 2016-2018. They also support the calculation of air-water N2O and CH4 fluxes and of the marshland greenhouse gas budget., This research was funded by the Organismo Autonomo de Parques Nacionales (Spanish Ministry for Agriculture, Food and Environment), project 1539/2015. Fiz F. Pérez was supported by the FICARAM+ project (PID2023-148924OB-I00) funded by MICIU/AEI/10.13039/501100011033 and FEDER, UE. S. Flecha was hired under the Generation D initiative, promoted by Red.es (Ministry for Digital Transformation and the Civil Service), financed by the Recovery, Transformation and Resilience Plan through the European Union's NextGeneration funds (MMT24-PTI+OCEANS-01). M. de la Paz acknowledges support from the Spanish Ministry of Science under grant PTA2024-025174-I., - Dissolved N2O and CH4: static headspace equilibration gas chromatography on an Agilent 7890 GC with an Electron Capture Detector (N2O) and a Flame Ionization Detector (CH4) (de la Paz et al. 2015)., Peer reviewed

European Commission National Natural Science Foundation of China Associazione Italiana per la Ricerca sul Cancro Instituto de Salud Carlos III Worldwide Cancer Research Fundación Científica Asociación Española Contra el Cáncer Fundación Fero Cancer Council NSW (Australia) National Health and Medical Research Council (Australia) German Research Foundation Shanghai Municipal Education Commission Ministero della Salute, Peer reviewed

Experimental Section: Chemicals and reagents; Instrumentation; Chiral activity characterization; Electrochemical monitoring of CISS effect; Atomic force microscopy measurements. -- Supporting Figures: Fig. S1. Linear dependence of peak current on scan rate for L-GeCys; Fig. S2. 50 cycles of cyclic voltammograms over L-GeCys; Fig. S3. Electrochemical impedance spectroscopy with L-AAO; Fig. S4. Bode plots of Log Z vs. Log Frequency; Fig. S5. Control experiment for a-GeCys; Fig. S6. Bode plots, impedance modulus ratio, and cyclability for isolated L-Cys; Fig. S7. Multi-cycle stability test for L-GeCys; Fig. S8. Short-term stability test for L-GeCys; Fig. S9. KPFM images and surface potentials of L-GeCys; Fig. S10. KPFM images and surface potentials of 2D–GeH. -- Supporting Table: Table S1. Auc and chemical bond contributions of L-GeCys, Peer reviewed

The dataset comprises processed peptidomic outputs generated from Thermo Scientific Orbitrap Exploris 240 LC-MS/MS analyses. Spectra were analysed using PEAKS Studio 11 (Bioinformatics Solutions Inc.) for de novo sequencing and database matching against in-house protein libraries containing bovine casein variants and porcine endogenous proteins. The dataset contains peptide-level and protein-level identification tables exported from PEAKS Studio. Data include peptide sequences, protein accessions, confidence scores (-10LgP), peptide abundances, retention times, mass-to-charge ratios, protein coverage values, and protein abundance measurements. The dataset was generated to evaluate the contribution of dietary and endogenous proteins to the peptide pool reaching the distal ileum and to investigate how seaweed-derived polysaccharides affect protein digestion under in vivo conditions., [Code Description] CAS Casein CAS_AG75 Casein:Agar (75:25) CAS_ALG75 Casein:Alginate (75:25) CAS_CAR75 Casein:Carrageenan (75:25) CAS_CEL75 Casein:Cellulose (75:25) WPI Whey Protein Isolate WPI_AG75 Whey Protein Isolate:Agar (75:25) WPI_ALG75 Whey Protein Isolate:Alginate (75:25) WPI_CAR75 Whey Protein Isolate:Carrageenan (75:25) WPI_CEL75 Whey Protein Isolate:Cellulose (75:25), This dataset contains peptide- and protein-level identifications obtained from LC-MS/MS analysis of ileal digesta samples collected from pigs fed diets containing milk proteins and seaweed-derived polysaccharides. The processed data include peptide sequences, protein assignments, confidence scores, abundance measurements, and protein coverage information., European Research Council (ERC), Project 101086483 – PRODIGEST – ERC-2022-COG: Nanostructure formation during food protein digestion and influence on intestinal transport., Peer reviewed

This PDF file includes: Figs. S1 to S26. -- Tables S1 to S4, Peer reviewed

Incubation with a non-toxic dose of etomoxir and macrophage-conditioned medium does not affect CD133 expression or self-renewal of PDAC cells, European Commission National Natural Science Foundation of China Associazione Italiana per la Ricerca sul Cancro Instituto de Salud Carlos III Worldwide Cancer Research Fundación Científica Asociación Española Contra el Cáncer Fundación Fero Cancer Council NSW (Australia) National Health and Medical Research Council (Australia) German Research Foundation Shanghai Municipal Education Commission Ministero della Salute, Peer reviewed