Resultados de investigación

[Organism] Homo sapiens, [Experiment type] Expression profiling by array, [Overall design] Adipose tissue and blood samples were obtained from 27 children, 14 obese (BMI adjusted for age and sex z score > 2) and 13 non obese undergoing appendix surgery. About 400 mg of adipose tissue was taken and immediately immersed in RNAlater solution and stored at -80°C for gene expression analysis. Informed consent was obtained from all patients after the nature of the study was explained, and the experimental design was approved, from an ethical and scientific standpoint, by the Hospital’s Ethical Committee responsible for research., [Platforms (1)] GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array, [Relations] BioProject PRJNA103477, 1. GSM243215 Adipose tissue_423_control_rep1. [Source name] Omental adipose tissue, normal weight, child. [Characteristics] tissue: omental adipose group: normal weight gender: M age: 10 year z score bmi: -1.45, 2. GSM243216 Adipose tissue_425_control_rep2. [Source name] Omental adipose tissue, normal weight, child. [Characteristics] tissue: omental adipose group: normal weight gender: M age: 7 year z score bmi: 0.49, 3. GSM243217 Adipose tissue_426_control_rep3. [Source name] Omental adipose tissue, normal weight, child. [Characteristics] tissue: omental adipose group: normal weight gender: F age: 8 year z score bmi: 0.12, 4. GSM243218 Adipose tissue_427_control_rep4. [Source name] Omental adipose tissue, normal weight, child. [Characteristics] tissue: omental adipose group: normal weight gender: M age: 10 year z score bmi: -1.81, 5. GSM243219 Adipose tissue_428_control_rep5. [Source name] Omental adipose tissue, normal weight, child. [Characteristics] tissue: omental adipose group: normal weight gender: M age: 7 year z score bmi: -0.64, 6. GSM243220 Adipose tissue_429_control_rep6. [Source name] Omental adipose tissue, normal weight, child. [Characteristics] tissue: omental adipose group: normal weight gender: M age: 7 year z score bmi: -0.36, 7. GSM243225 Adipose tissue_418_obese_rep1. [Source name] Omental adipose tissue, obese, child. [Characteristics] tissue: omental adipose group: obese gender: M age: 11 years z score bmi: 2, 8. GSM243226 Adipose tissue_419_obese_rep2. [Source name] Omental adipose tissue, obese, child. [Characteristics] tissue: omental adipose group: obese gender: M age: 9 years z score bmi: 4.25, 9. GSM243227 Adipose tissue_420_obese_rep3. [Source name] Omental adipose tissue, obese, child. [Characteristics] tissue: omental adipose group: obese gender: M age: 12 years z score bmi: 2.36, 10. GSM243228 Adipose tissue_421_obese_rep4. [Source name] Omental adipose tissue, obese, child. [Characteristics] tissue: omental adipose group: obese gender: M age: 9 years z score bmi: 4.25, 11. GSM243275 Adipose tissue_422_obese_rep5. [Source name] Omental adipose tissue, obese, child. [Characteristics] tissue: omental adipose group: obese gender: M age: 10 years z score bmi: 2.72, Characterization of genes associated with adipose tissue is key to understanding the pathogenesis of obesity and developing treatments for this disorder. Differential gene expression in the adipose tissue has been described in adulthood but none studies have been developed on childhood. The purpose of this study was to compare gene expression in omental adipose tissue from obese prepubertal and normal weight children. We selected 5 obese (BMI adjusted for age and sex z score >2) and 6 normal weight children. RNA was extracted from omental adipose tissue biopsies and cRNA was hybridizated on the human genome U133 Plus 2.0 Arrays (Affymetrix®). Microarray experiments were performed for each sample, and selected group of gene expression values were confirmed with real-time RT-PCR in 10 obese and 10 normal weigth prepubertal children. 1276 genes were found to be differentially expressed at P<0.05. Of those differential genes, 201 were upregulated (Fc>2) and 42 were downregulated (Fc<-2). Genes involved in metabolic and signalling pathways were altered in childhood obesity. Keywords: disease state analysis

[Organism] Rattus norvegicus, [Experiment type] Expression profiling by array, [Overall design] Breast tumors were induced with a single oral dosage of 7,12-dimethylbenz(alpha)anthracene (100 mg/kg body weight) in female Sprague-Dawley rats. Gene expression analysis was performed in paired samples as follows: DMBA final trucut tumor vs initial trucut tumor (DMBA final vs basal). For this assay, 5 samples were chosen according to histopathologic criteria (Bloom-Richardson grade II). Gene expression profiling was carried out using Affymetrix’s GeneChip technology, using the Rat Genome 230 2.0 array from this provider. All the protocols and apparatus were recommended by Affymetrix. Total RNA from frozen mammary tumors was extracted by RNeasy Mini kit and homogenized by QIAshredder columns according to manufacturer’s instructions. The quality and quantity of the obtained RNA was checked out through agarose electrophoresis and later spectrophotometry at 260/280 nm. Biotinylated cRNA was synthesized following the IVT labeling kit from Affymetrix and purified by the GeneChip Sample Cleanup Module from Affymetrix. The quality and quantity of the obtained cRNA was again checked out through agarose electrophoresis and posterior spectrophotometry at 260/280 nm. After hybridization, slides were washed and scanned following the manufacturer’s standard protocol. Intensity values were normalized by Robust Multichip Average method and subsequently these were filtered to remove the control sequences and those with a hybridization signal close to background. The spike controls were: BioB, BioC, BioD and Cre; because BioB was the least abundant in the samples, it was used to estimate the sensitivity of the experiment. The housekeeping control was GAPDH. After non-supervised clustering using Pearson correlation coefficient, statistical significance of gene expression was estimated by Student’s T test for paired samples, using GeneSpring GX 7.3 software (Agilent)., [Platforms (1)] GPL1355 [Rat230_2] Affymetrix Rat Genome 230 2.0 Array, [Relations] BioProject PRJNA115167, 1. GSM398893 Breast tumor biopsy_DMBA_07SE115. [Source name] Breast tumor biopsy, DMBA, basal moment. [Characteristics] strain: Sprague-Dawley gender: female age: 12 weeks tissue: Breast tumor. Trucut Biopsy of breast tumor chemically induced with 7,12-dimethylbenz(a)anthracene. Such biopsy was taken when tumor had 2cm3 volume. biopsy dimensions: 0.7 cm x 2.5 mm treatment: none sample: 31D3 (basal), 2. GSM398894 Total breast tumor_DMBA_07SE130. [Source name] Total breast tumor, DMBA, final moment. [Characteristics] strain: Sprague-Dawley gender: female age: 19 weeks tissue: Breast tumor. Breast tumor chemically induced with 7,12-dimethylbenz(a)anthracene at the moment of sacrifice. treatment: none sample: 31D3 (final), 3. GSM399028 Breast tumor biopsy_DMBA_07SE116. [Source name] Breast tumor biopsy, DMBA, basal moment. [Characteristics] strain: Sprague-Dawley gender: female age: 12 weeks tissue: Breast tumor. Trucut Biopsy of breast tumor chemically induced with 7,12-dimethylbenz(a)anthracene. Such biopsy was taken when tumor had 2cm3 volume. biopsy dimensions: 0.7 cm x 2.5 mm treatment: none sample: 32D1 (basal)., 4. GSM399030 Total breast tumor_DMBA_07SE131. [Source name] Total breast tumor, DMBA, final moment. [Characteristics] strain: Sprague-Dawley gender: female age: 19 weeks tissue: Breast tumor. Breast tumor chemically induced with 7,12-dimethylbenz(a)anthracene at the moment of sacrifice. treatment: none sample: 32D1 (final), 5. GSM399430 Breast tumor biopsy_DMBA_07SE117. [Source name] Breast tumor biopsy, DMBA, basal moment. [Characteristics] strain: Sprague-Dawley gender: female age: 12 weeks tissue: Breast tumor. Trucut Biopsy of breast tumor chemically induced with 7,12-dimethylbenz(a)anthracene. Such biopsy was taken when tumor had 2cm3 volume. biopsy dimensions: 0.7 cm x 2.5 mm treatment: none sample: 37I1 (basal), 6. GSM399433 Total breast tumor_DMBA_07SE132. [Source name] Total breast tumor, DMBA, final moment. [Characteristics] strain: Sprague-Dawley gender: female age: 19 weeks tissue: Breast tumor. Breast tumor chemically induced with 7,12-dimethylbenz(a)anthracene at the moment of sacrifice. treatment: none sample: 37I1 (final), 7. GSM399434 Breast tumor biopsy_DMBA_07SE118. [Source name] Breast tumor biopsy, DMBA, basal moment. [Characteristics] strain: Sprague-Dawley gender: female age: 12 weeks tissue: Breast tumor. Trucut Biopsy of breast tumor chemically induced with 7,12-dimethylbenz(a)anthracene. Such biopsy was taken when tumor had 2cm3 volume. biopsy dimensions: 0.7 cm x 2.5 mm treatment: none sample: 50I1 (basal), 8. GSM399456 Total breast tumor_DMBA_07SE133. [Source name] Total breast tumor, DMBA, final moment. [Characteristics] strain: Sprague-Dawley gender: female age: 19 weeks tissue: Breast tumor. Breast tumor chemically induced with 7,12-dimethylbenz(a)anthracene at the moment of sacrifice. treatment: none sample: 50I1 (final), 9. GSM399460 Breast tumor biopsy_DMBA_07SE119. [Source name] Breast tumor biopsy, DMBA, basal moment. [Characteristics] strain: Sprague-Dawley gender: female age: 12 weeks tissue: Breast tumor. Trucut Biopsy of breast tumor chemically induced with 7,12-dimethylbenz(a)anthracene. Such biopsy was taken when tumor had 2cm3 volume. biopsy dimensions: 0.7 cm x 2.5 mm treatment: none sample: 52I4 (basal), 10. GSM399466 Total breast tumor_DMBA_07SE134. [Source name] Total breast tumor, DMBA, final moment. [Characteristics] strain: Sprague-Dawley gender: female age: 19 weeks tissue: Breast tumor. Breast tumor chemically induced with 7,12-dimethylbenz(a)anthracene at the moment of sacrifice. treatment: none sample: 52I4(final), The aim of this study was to compare the gene expression profile changes of DMBA-induced rat breast tumors from an initial stage to the moment of sacrifice. To this end, a cDNA microarray was performed (Affymetrix’s Rat Genome 230 2.0 array). This gene expression study was carried out on the umor biopsy samples and compared with matched tumor biopsy samples once the study ended (7 weeks after initial biopsy).

CSV file with scientific output of 102 countries (rows) by 27 major Scopus/SCImago subject areas, from 1996 to 2006. Data extracted from SCImago Journal & Country Rank (scimagojr.com).

[Organism] Rattus norvegicus, [Experiment type] Expression profiling by array, [Overall design] A single enema of 10mg of TNBS in 50% ethanol was administered to rats at day 0. Samples were recovered at days 2, 5, 7 and 14. According to inflammatory markers (myeloperoxidase activity, body weigh loss, colonic weigh/length ratio) we selected three replicates at each time point for the genimoc analysis, and 6 healthy control rats that received a saline enema. RNA was extracted from homogenized full-thickness colonic tissues in Trizol® reagent (Invitrogen) and purified with RNeasy affinity columns (Qiagen), according to manufacturer´s protocol. The microarray analysis was performed by Progenika Biopharma (Bilbao, Spain) on GeneChip® Rat Genome 230 2.0 Array (Affymetrix). All sample labeling (biotin), hybridization, staining and scanning procedures were carried out using Affimetrix, standard protocols (www.affymetrix.com). Normalization was carried out using Bioconductor sofware (affyPLM package)., [Platforms] GPL1355 [Rat230_2] Affymetrix Rat Genome 230 2.0 Array, [Relations] BioProject PRJNA102933, 1. GSM235052 Healthy control, replicate 1. [Characteristics] Sprague-Dawley female rats Tissue: full-thickness colon Rat received water, 2. GSM235053 Healthy control, replicate 2. Characteristics Sprague-Dawley female rats Tissue:full-thickness colon Rat received water, 3. GSM235055 Helathy control, replicate 3. [Characteristics] Sprague-Dawley female rats Tissue: full-thickness colon Rat received water, 4.GSM235056 Healthy control, replicate 4. [Characteristics] Sprague-Dawley female rats Tissue: full-thickness colon Rat received water, 5. GSM235057 Healthy control, replicate 5. [Characteristics] Sprague-Dawley female rats Tissue: full-thickness colon Rat received water, 6. GSM235058 Healthy control, replicate 6. [Characteristics] Sprague-Dawley female rats Tissue: full-thickness colon Rat received water, 7. GSM235552 TNBS day 2, replicate 1. [Characteristics] Sprague-Dawley female rat Tissue: full-thickness colon Rat received an enema of 10mg TNBS in 50% ethanol at day 0, 8. GSM235558 TNBS day 2, replicate 2. [Characteristics] Sprague-Dawley female rat Tissue: full-thickness colon Rat received an enema of 10mg TNBS in 50% ethanol at day 0, 9. GSM235559 TNBS day 2, replicate 3. [Characteristics] Sprague-Dawley female rat Tissue: full-thickness colon Rat received an enema of 10mg TNBS in 50% ethanol at day 0, 10. GSM235643 TNBS day 5, replicate 1. [Characteristics] Sprague-Dawley female rat Tissue: full-thickness colon Rat received an enema of 10mg TNBS in 50% ethanol at day 0, 11. GSM235745 TNBS day 5, replicate 2. [Characteristics] Sprague-Dawley female rat Tissue: full-thickness colon Rat received an enema of 10mg TNBS in 50% ethanol at day 0, 12. GSM235832 TNBS day 5, replicate 3. [Characteristics] Sprague-Dawley female rat Tissue: full-thickness colon Rat received an enema of 10mg TNBS in 50% ethanol at day 0, 13. GSM235872 TNBS day 7, replicate 1. [Characteristics] Sprague-Dawley female rat Tissue: full-thickness colon Rat received an enema of 10mg TNBS in 50% ethanol at day 0, 14. GSM235873 TNBS day 7, replicate 2. [Characteristics] Sprague-Dawley female rat Tissue: full-thickness colon Rat received an enema of 10mg TNBS in 50% ethanol at day 0, 15. GSM236126 TNBS day 7, replicate 3.[Characteristics] Sprague-Dawley female rat Tissue: full-thickness colon Rat received an enema of 10mg TNBS in 50% ethanol at day 0, 16. GSM236922 TNBS day 14, replicate 1. [Characteristics] Sprague-Dawley female rat Tissue: full-thickness colon Rat received an enema of 10mg TNBS in 50% ethanol at day 0, 17. GSM236924 TNBS day 14, replicate 2. [Characteristics] Sprague-Dawley female rat Tissue: full-thickness colon Rat received an enema of 10mg TNBS in 50% ethanol at day 0, 18. GSM236925 TNBS day 14, replicate 3. [Characteristics] Sprague-Dawley female rat Tissue: full-thickness colon Rat received an enema of 10mg TNBS in 50% ethanol at day 0, Trinitrobenzenesulfonic acid (TNBS) rat colitis is one of the most widely used models of inflammatory bowel disease, a condition whose etiology and pathophysiology are incompletely understood. We have characterized the model at the genomic level following a longitudinal approach.

The aim of this research is to determine how the most sustainable brands in Italy and Spain developed communication and awareness-raising actions during the COVID-19 pandemic and whether these companies were truly involved in raising public awareness of the pandemic. We specifically focus on sustainable companies, as health communication is considered to be an important corporate social responsibility task., This research was supported by MCIN/AEI/10.13039/501100011033 and ERDF “A way of making Europe” under grant CONFIA (PID2021-122916NB-I00).

Bases de datos utilizadas en un trabajo de investigación, 6 bases de datos en SPSS

In this empirical study, the relationships between religiosity, spirituality, a sense of life, searching for meaning, and a crisis of meaning are explored in relation to engagement and satisfaction with learning among university students. The results of the study, conducted with Spanish university students, revealed a committed sample to learning, not very satisfied with it, whose life has mean-ing and/or is in the process of seeking it, with a much lower incidence of a crisis of meaning. The presence of meaning in life and the crisis of meaning were shown to be related to vigor, dedica-tion, and absorption in learning, all of which are expressions of commitment to it. Engagement with learning was also found to be linearly and positively related to a sense of life. Extrinsic and in-trinsic religious orientations exhibited a similar pattern of relationships, positively correlating with vigor, absorption, and learning engagement, and remaining independent of dedication to learning and satisfaction with it. The results suggest that education should focus on aspects and spiritual practices that have personal meaning for students. The text emphasizes the importance of foster-ing an active and attentive disposition in students to engage in activities that provide meaning. It also suggests that curriculum content should relate to students’ interests and concerns, irrespective of their religious or spiritual dimensions.