Resultados de investigación

This dataset corresponds to the article "Biocompatible gemcitabine-based nanomedicine engineered by Flow Focusing® for efficient antitumor activity." It includes experimental data on the preparation and characterization of gemcitabine-loaded PLGA nanoparticles produced by two synthesis methods: double emulsion/solvent evaporation (DE/SEV) and Flow Focusing® (FF). The data encompass zeta potential, drug loading, encapsulation efficiency, drug release profiles and in vitro antitumor activity against MCF-7 cells., A single Excel file with each sheet collecting the data corresponding to a figure or table: -Figure 3: Data corresponding to manuscript Figure 3 on NPs encapsulation efficiency and gemcitabine loading obtained with both methodologies using different values of initial loading. -Figure 4: Data corresponding to manuscript Figure 4 on the zeta potential of NPs produced with both methodologies under different pH values. -Figure 5: Data corresponding to manuscript Figure 5 on the drug release profiles from NPs elaborated with both methodologies. -Figure 6: Data corresponding to manuscript Figure 6 on the cytotoxic effect on MCF-7 human breast adenocarcinoma cells of free gemcitabine, blank and gemcitabine-loaded NPs produced with both methodologies.

Development and characterization of cannabinoid-loaded receptor-targeted nanoparticles, dual-functionalized with fluorophore probes. The data collects the analysis of the physicochemical properties of the nanoparticles, their capacity to target and exert cytotoxic activity on tumor cells based on their surface modification with transferrin, and the analysis of their cell internalization and subsequent tracking within the cell, monitoring both the nanoparticle core and the encapsulated cargo. The data were generated within the Proyecto de Excelencia de la Junta de Andalucía No. P09-CTS5029, and the aid of a postdoctoral fellowship Contrato Puente Postdoctoral (USE-12003-C) from the V Plan Propio de Investigación of the Vicerrectorado de Investigación from the Universidad de Sevilla., A single Excel file with each sheet collecting the data corresponding to a figure or table: -Table 1: data corresponding to manuscript Table 1 on the physicochemical characterization of the formulations -Figure 2: data corresponding to manuscript Figure 2 displaying the drug release profile of the formulations -Figure 3: data corresponding to manuscript Figure 3 displaying the cell viability results after incubation with the formulations for 2h, 24h and 48h -Figure 4: data corresponding to manuscript Figure 4 displaying the % of fluorescence recovered from the nanoparticle core (FITC signal) and the encapsulated cargo (NR and Rh signal) for either plain and transferrin-modified nanoparticles, with or without the use of endocytosis specific inhibitors. -Figure 5: data corresponding to manuscript Figure 5 b and c, providing quantitative data of nanoparticle cell internalization and intracellular tracking as a function of the fluorescence intensity obtained from both the nanoparticle core (FITC signal) and the delivered cargo (Nile Red and Rhodamine B signal), and the colocalization of both, for both plain and transferrin-modified nanoparticles.

This dataset corresponds to the article "Lipid nanoparticles as an emerging platform for cannabinoid delivery: physicochemical optimization and biocompatibility," published in Drug Development and Industrial Pharmacy. It includes experimental data on the development of CB13-loaded lipid nanoparticles (LNPs) prepared using the emulsification-solvent evaporation method. Data include particle size, polydispersity index (PDI), zeta potential, encapsulation efficiency, and stability studies. Biocompatibility was evaluated through cytotoxicity assays performed on NIH 3T3, HEK 293T, and Caco-2 cell lines., A single Excel file with each sheet collecting the data corresponding to a figure or table: -Figure 1: Data corresponding to manuscript Figure 1 on particle size zeta potential values of the formulations as a function on the different parameters assayed. -Figure 4: Data corresponding to manuscript Figure 4 on colloidal stability of the assayed formulations in biorelevant fluids. -Figure 5: Data corresponding to manuscript Figure 5 on the cytotoxic effect on NIH3T3 cells, HEK293T cells and Caco-2 cells of the different types of NPs assayed. -Figure A1: Data corresponding to manuscript Figure A1 on storage stability of the selected formulations.

Dataset acquired for the study published in the article: “Improving the performance of felt-based living wall systems in terms of irrigation management”. This work aims to improve an existing commercial system (Fytotextile) in order to optimise water retention and vegetation performance in harsh climate conditions. Therefore, three evolutions of the Fytotextile system were tested in terms of water retention capacity, drainage and vegetation performance., Water retention capacity, the mean total volumes and maximum flows of water drained in a day from the 4 types of Fytotextile living walls and performance of the plants

Base de datos municipalizada para el conjunto de España con información para la identificación y caracterización de los sistemas agroalimentarios territorializados., Información municipalizada sobre: superficie de áreas protegidas, % superficie municipal de áreas protegidas, Superficie Agraria Útil (SAU), % de superficie municipal correspondiente a Superficie Agraria Útil (SAU), Superficie de olivar (ha), % de superficie municipal correspondiente a olivar, Superficie de viñedo (ha), % de superficie municipal correspondiente a viñedo, Superficie de arrozales (ha), % de superficie municipal correspondiente a arrozales, Superficie de mosaico de cultivos (ha), % de superficie municipal correspondiente a mosaico de cultivos, Superficie de frutales (ha), % de superficie municipal correspondiente a frutales, Superficie de cereales de secano (ha), % de superficie municipal correspondiente a cereales de secano, Superficie agroforestal (ha), % de superficie municipal agroforestal, Superficie de prados (ha), % de superficie municipal de prados, número de grupos de desarrollo rural, número de Denominaciones de Origen Protegidas.

Los profundos cambios sociales surgidos en los países occidentales en las últimas décadas han retrasado la adquisición de los roles adultos, e hitos que generaciones anteriores alcanzaban en la década de los 20, se adquieren ahora cerca de los 29. Así, ha surgido un nuevo periodo evolutivo denominado adultez emergente, que transcurre entre los 18 y los 30 años, que tiene características únicas y distintivas y que exige un análisis profundo y singular. Los proyectos de investigación Transición a la Adultez en España TAE I y TAE II o, lo que es lo mismo, La Transición a la Adultez en España: Estudio Sobre las Claves del Ajuste Psicosocial y Fundamentos para su Intervención Preventiva (EDU201345687-R; Estudio Longitudinal Secuencial Sobre la Transición a la Adultez en España (RTI2018-097405-B-I00); y Factores de Riesgo y Protección en la Adultez Emergente. Un estudio longitudinal-secuencial (PROYEXCEL_00766) han supuesto un primer acercamiento en nuestro país al estudio del desarrollo de las personas durante su adultez emergente, paliando la ausencia de investigaciones en España que, desde un punto de vista psicológico, estudiaran esta etapa. Los proyectos TAE tienen como objetivo principal la identificación de los factores de riesgo y protección que intervienen en el desarrollo de las personas jóvenes desde una perspectiva de género. En concreto esta Base de Datos contiene aquellos utilizados en el artículo que se reseña más adelante, y hace referencia a variables de funcionamiento familiar y de ajuste personal. En este artículo se seleccionó a la muestra de la provincia de Sevilla y se comparó a quienes estudian en la US y quienes lo hacen en la EU Osuna. Así pudimos comparar a el estudiantado de Osuna, que realizó la recogida de datos de 2020 ANTES de que se decretara la pandemia y el confinamiento con el estudiantado de Osuna de 2015 y el de la US de 2020, cuyos datos se recogieron en otoño de 2020, tras confinamiento y EN PLENA pandemia con sus homólogos de 2015. Así, pudimos analizar el impacto en ajuste personal y funcionamiento familiar que supuso la pandemia comparando el grupo de la US con el grupo control de la EUO., Variables sociodemográficas sexo, edad, situación laboral, ingresos familiares, convivencia, Variables resumen creadas Florecimiento, Implicación parental, Cariño parental, distress, Variables de interacción

Datos extraídos de la web de sembramedia con fecha 20 de febrero de 2018 con el objetivo de analizar las características de los medios digitales de habla hispana, Tipo de organización Area de Cobertura Tipo de Contenido Género Periodístico Técnicas Periodísticas empleadas Verificación de datos factchecking Año de fundación Plataforma tecnológica Miembros o subscriptores Contenido activista Transparencia Origenes de los contenidos Origenes_Fundador Equipo en modalidad fulltime Cantidad de personas abocadas a producir contenido Fuentes de ingresos Tráfico páginas vistas por mes Última fecha de modificación

Data was acquired for the study published in the article titled: Anti-obesogenic effect of lupin-derived protein hydrolysate through modulation of adiposopathy, insulin resistance and gut dysbiosis in a diet-induced obese mouse. This study examines the effects of a lupin protein hydrolysate (LPH) on obesity, adipose tissue dysfunction and gut dysbiosis in obese mice. LPH was characterized and protein concentration, ashes, moisture, fiber, fats, soluble sugars and phenol content were measured as described. Amino acid composition was determined using standard amino acid mix solution. The molecular weight profile was obtained by molecular exclusion chromatography. Eight-week-old male C57BL6/N mice were housed under standard conditions and mice were randomly assigned to three groups: mice fed a standard diet (SD), a high-fat diet (HFD) or an HFD and treated intragastrically with LPH (HFD+LPH) at a dose of 100mg/kg five days a week for 12 weeks. The SD and HFD groups were treated with vehicle under the same conditions as the HFD+LPH group. Food consumption and individual body weight were measured weekly. Animals were sacrificed with an intraperitoneal injection of sodium thiopental and blood was collected by cardiac puncture. Epidydimal adipose tissue (EpiWAT) was dissected, snap-frozen and stored at -80ºC until use or fixed in a 4% paraformaldehyde (PFA) solution. Stool was collected prior to euthanasia and stored at -80ºC. Serum biochemical profile was measured by chemoluminiscence in the Cobas Integra 400 (Roche Diagnostics, Indianapolis, IN, USA) at the Estación Biológica de Doñana (EBD-CSIC, Seville, Spain). Serum concentrations of leptin, adiponectin and insulin were quantified using enzyme-linked immunosorbent assays (ELISA) kits. Adipose tissue samples fixed in 4% PFA were dehydrated, embedded in paraffin blocks and sliced 4μm thick, stained with hematoxylin-eosin and cover mounted for posterior observation. Photomicrographs were obtained using Leica THUNDER microscope and Leica Application Suite X software. Adipocyte morphology and number of crown-like structures was blinded analyzed using ImageJ v1.53h public software. High throughput 16S rRNA gene amplicon analysis was performed using total DNA extracted from stool. 16S rRNA V3–V4 region was sequenced using MiSeq protocol and instruments. The raw sequences were processed using mothur., Dataset entitled “hydrolysate_composition.xlsx” includes macromolecular and aminoacidic composition (first sheet) and chromatogram information (second sheet) of LPH obtained from three independent measurements. Dataset entitled “obesity_markers.xlsx” includes experimental group and numerical data (columns) obtained from the measurement of parameters from animals (rows) used in the present study.

Background Antibiotics could modify multidrug-resistant microorganisms (MDRO) populations in urban wastewater. Water treatment plays a major role in their control. This study aimed to explain the characteristics of multidrug-resistant Gram-negative bacteria in the wastewater of a city in southern Spain, the reduction achieved by treatment and the influence of human antibiotic prescriptions. Methods We collected 24-hour composite water samples from four wastewater treatment plants (WWTPs) over one year (2021-2022) and cultured them quantitatively. All acquired ESBL/carbapenemase isolates were sequenced and genomes were compared with those of clinical isolates. Linear mixed model regression analyses were performed to assess the WWTP effect and the association with human antibiotic prescriptions. Findings Aeromonas predominated among the MDROs and KPC-2 was the main carbapenemase detected in the influents. The 44% and 30% of E. coli and K. pneumoniae influent isolates, respectively, belonged to high-risk clones; 10·6% of Enterobacteriaceae could be matched to clinical isolates and a strain from a current hospital outbreak was found in raw samples. WWTPs reduced ESBL and carbapenemase producers by 1·4-log and 1·8-log, respectively. New MDROs and persistence of certain strains were detected in effluents. Quinolone and cephalosporin prescriptions, flow-rate and population density were associated with higher OXA-48 producer counts. Interpretation Despite the reduction, additional technologies should be implemented in WWTPs receiving hospital discharges. Due to the predominance of environmental species, culture and metagenomic approaches should be combined to differentiate human and sewages origin for antibiotic resistance monitoring. Antibiotic stewardship could be a strategy to reduce the release of MDROs., 1. Excel con el registro de aislados bacterianos y metadata 2. Word con los cálculos estadísticos

Estudio experimental sobre la correlación de las variables psicofisiológicas, HR, GSR y T, con el arousal previamente, en imágenes susceptibles de usar en comunicación., Aaa_____ son los registros brutos de las tres variables utilizadas Documentos Excel con los datos de todos los participantes, solo la parte de corresponder a los registros tomados durante la estimulación con cada imagen. Documentos de SPSS son los estudios estadísticos.