Resultados de investigación

The dataset includes the raw data from the clinical score and flow cytometry analyzes carried out in the work titled " Melatonin synergistically potentiates the effect of methylprednisolone on reducing neuroinflammation in the experimental autoimmune encephalomyelitis mouse model of multiple sclerosis". This study shows the protective synergistic effect of co-treatment with melatonin and methylprednisolone on reducing the severity of EAE by decreasing CD4+ lymphocytes, B cells, macrophages and dendritic cells in the CNS, as well as modulating the population of infiltrated T and B cells toward regulatory phenotypes to the detriment of pro-inflammatory effector functions. In addition, treatment with melatonin from the clinical onset of EAE improves the natural course of the EAE and the response to a subsequent treatment with methylprednisolone in a later relapse of the disease. Eight-week-old female C57BL/6N mice were immunized immunization with 100 μg of MOG35–55 (Cambridge Research Biochemicals, Cleveland) emulsified in CFA (Sigma) containing 50 μg of heat-killed Mycobacterium tuberculosis (H37Ra, ATCC 25177) by subcutaneous injection in both hind legs and two doses of intraperitoneal pertussis toxin (200 ng/day) (List Labs, California) on days 0 and 2 post-induction. Animals were randomly divided to receive melatonin at a concentration of 80 mg/kg and/or methylprednisolone at a concentration of 40 or 160 mg/kg in different treatment regimens. Mice were sacrificed at the peak of the disease (day 15 after induction) and after perfusion, the CNS was collected, homogenized and enzymatically dissociated with 1.87 mg/ml of collagenase IV (Worthington) and 0.25 mg/ml of DNase I (AppliChem) for 35 min at 37°C to obtain a suspension of single cells. Subsequently, a 37%:70% discontinous percoll gradient was carried out to isolate CNS-infiltrating mononuclear cells. To assess the profile of infiltrated immune cells in the CNS, cells were stained for the following antibodies against surface markers: CD45, CD4, CD8α, CD19, CD11b, CD11c, CD44, CD62L, B220, CD138, PD-1 (CD279), CTLA-4 (CD152), FAS (CD95), and CD25. To identify Treg and analyze intracellular production of TNF, IFN-γ and IL-10, after surface staining, cells were fixed and permeabilized using the The dataset includes the raw data from the clinical score and flow cytometry analyzes carried out in the work titled " Melatonin synergistically potentiates the effect of methylprednisolone on reducing neuroinflammation in the experimental autoimmune encephalomyelitis mouse model of multiple sclerosis". This study shows the protective synergistic effect of co-treatment with melatonin and methylprednisolone on reducing the severity of EAE by decreasing CD4+ lymphocytes, B cells, macrophages and dendritic cells in the CNS, as well as modulating the population of infiltrated T and B cells toward regulatory phenotypes to the detriment of pro-inflammatory effector functions. In addition, treatment with melatonin from the clinical onset of EAE improves the natural course of the EAE and the response to a subsequent treatment with methylprednisolone in a later relapse of the disease. Eight-week-old female C57BL/6N mice were immunized immunization with 100 μg of MOG35–55 (Cambridge Research Biochemicals, Cleveland) emulsified in CFA (Sigma) containing 50 μg of heat-killed Mycobacterium tuberculosis (H37Ra, ATCC 25177) by subcutaneous injection in both hind legs and two doses of intraperitoneal pertussis toxin (200 ng/day) (List Labs, California) on days 0 and 2 post-induction. Animals were randomly divided to receive melatonin at a concentration of 80 mg/kg and/or methylprednisolone at a concentration of 40 or 160 mg/kg in different treatment regimens. Mice were sacrificed at the peak of the disease (day 15 after induction) and after perfusion, the CNS was collected, homogenized and enzymatically dissociated with 1.87 mg/ml of collagenase IV (Worthington) and 0.25 mg/ml of DNase I (AppliChem) for 35 min at 37°C to obtain a suspension of single cells. Subsequently, a 37%:70% discontinous percoll gradient was carried out to isolate CNS-infiltrating mononuclear cells. To assess the profile of infiltrated immune cells in the CNS, cells were stained for the following antibodies against surface markers: CD45, CD4, CD8α, CD19, CD11b, CD11c, CD44, CD62L, B220, CD138, PD-1 (CD279), CTLA-4 (CD152), FAS (CD95), and CD25. To identify Treg and analyze intracellular production of TNF, IFN-γ and IL-10, after surface staining, cells were fixed and permeabilized using the FoxP3/transcription factor staining buffer set (eBioscience) and stained with anti-FoxP3 or anti-TNF, -IFN-γ and -IL-10, respectively. The intracellular production of cytokines was carried out in cells cultured at 2.5 × 106 cells/ml with RPMI 1640 supplemented with 5% fetal bovine serum, 1% L-glutamine and 1% of penicillin/streptomycin incubated in the presence or absence of Phorbol-Myristate-Acetate and Ionomycin (PMAI, Sigma) with brefeldin A (eBioscience) for 5 h. Dead cells were excluded from analyses using the LIVE/DEAD® Fixable Dead Cell Stain Kit (Invitrogen). FACS analysis was performed using a Cytek Aurora spectral cytometer, and data were analyzed using FlowJo software (Treestar)., The data set titled “Mel+MPD co-therapy.xlsx” includes numerical data from flow cytometry analysis (first sheet) and clinical score analysis (second sheet). A legend is also incorporated into the third sheet detailing the different experimental groups of the study.

The dataset includes the raw data from the weight, clinical score, and number of sequences carried out in the work titled "A lupin (Lupinus angustifolius) protein hydrolysate decreases the severity of experimental autoimmune encephalomyelitis, a preclinical model of multiple sclerosis". LPH reduced the clinical score of the mice compared to the control group, as well as the maximum and cumulative scores, without changing the day of onset of the symptoms while the therapeutic intervention did not significantly improve the severity of the disease. Fifty-six female C57BL/6N mice (8-weeks-old) from the University of Seville Animal Facility were housed under standard conditions with ad libitum access to water and food. For the induction of EAE, 100 µg of MOG35–55 peptide (Cambridge Research Biochemicals, Cambridge, UK) emulsified in complete Freud´s adjuvant (CFA) containing 4 mg/mL of heat-inactivated Mycobacterium tuberculosis (Sigma-Aldrich, St. Louis, MO, USA) was inoculated subcutaneously. Furthermore, two doses of 400 ng pertussis toxin (Enzo Life Sciences, Farmingdale, NY, USA) were administered intraperitoneally on days 0 and 2 post-induction (p.i.) to induce the disease. An EAE clinical sign score was assigned daily to each mouse as follows: 0 = no clin-ical sign; 1 = no tail tone; 2 = impaired righting reflex; 3 = paralysis of one hind limb; 4 = paralysis of both hind limbs; 5 = paralysis of both hind limbs and one forelimb; 6 = if the score was greater than 5 (including moribund or dead mouse). LPH sequencing was performed using nano high-performance liquid chromatography coupled to an Orbitrap Elite mass spectrometer. The BIOPEP-UWM database was used to identify the motifs with proven biological activity., The data set titled “LPH_EAE_Dataset.xlsx” includes numerical data from mice weight and clinical score, and the numbers of sequences with a determinate biological activity.

Reports related to the study published under the title "Unveiling protective mechanisms of wild olive (wild olive tree) oil in retinal pigment epithelial cells with hypertensive phenotype". This study was funded thanks to the project of the state plan PID2019-109002RB-I00 and the Junta de Andalucía ProyExcel_00516. In both projects, the beneficial properties of wild olive oil at the retinal level are described. Specifically, these data refer to the antioxidant properties of extracellular vesicles obtained from the plasma of animals treated with diets enriched in wild olive oil and their effect on retinal pigment epithelium cells subjected to a hypertensive phenotype. Dataset generated were collected between 2021 and 2024, part of which is the result of research stays abroad in collaboration with the Karolinska Institutet., Figura 1_ MTT and DCFHDA levels.pzfx: data corresponding to manuscript Figure 1 on cell viability and ROS. Figura 2_CAT.pzfx: data corresponding to Figure 2 in manuscript indicating protein and gene expression of CAT-1. Figura 2_Datos_vi.pzfx: data corresponding to Figure 2 in manuscript indicating L-arginine initial velocity. Figura 2_Transporte_articulo.pzfx: data corresponding to Figure 2 in manuscript indicating L-arginine transport and CAT-1 kinetic. Figura 3_DCFHDA_ATPCR.pzfx: data corresponding to Figura 3 of manuscript indicating DHFDA levels and WB and RT-PCR of angiotensin receptors. Figura 4_NADPH-MITOSOX.pzfx: data corresponding to Figure 4 of manuscript indicating activity and expression of NADPH oxidase and mitochondrial ROS. Figura 5_NO-Arginasas.pzfx: data corresponding to Figure 5 of manuscript indicating NO and ADMA values and protein and gene expression of arginases.

We have focused on the analyses of the most negative memories of a particular group of doctors related to their work experience. After interviewing 24 professionals of different specialties, their narratives were analyzed using the criteria of specificity, agency, and theme of the memory. Most memories were considered specific as they were vividly remembered with attention to detail. Men have more narratives linked to agency, while the majority of women develop narratives linked to communion. Furthermore, five clearly differentiated topics were observed regarding the professional’s degree of experience. A qualitative analysis of the memories of these professionals, as well as a description of the appropriation process of the discursive features is presented. The dimensions of analysis of the responses included in the Dataset are described in the Coding Manual., A psychologist specializing in human resources designed an ad hoc autobiographical interview. The intention of this interview was to both respond to the questions of the research and to learn about the memories related to their jobs, and learn how these memories have changed their lives. From the existing prompts, the following was the one chosen to be analyzed. “I would like you to recall a memory of your professional experience which you consider to be particularly negative, specifying the emotion with which you would describe it.” The interviews, conducted by the first author (S.A.S.) and principal researcher, had an average duration of 30minutes and were conducted in either the participant’s office or a meeting room in the healthcare agency. After the verbal transcription of the memories, two trained researchers analyzed the narratives following three steps and using version 15 of the program Atlas-Ti for the coding. In Figure 1, a summary of the process, which could be considered a mixed-method type study, is presented. First, following the categories of Conway and Pleydell-Pearce (2000) narratives were coded as specific, referring to events that once occurred at a particular time (“the night my first patient died”), or general, referring to events that occur regularly or multiple times (“when I was not allowed to do an intervention because the lack of funds”). Second, in order to categorize the orientation to agency the “Coding Autobiographical Episodes for Themes of Agency and Communion” (McAdams, 2001). Finally, the interviews were analyzed individually by three researchers and later collectively as a group looking for the most important issues remembered. In accordance with our aims, a thematic analysis was used for the qualitative interview’s analysis since it is a robust and parsimonious methodology of enquiry data (Braun and Clarke, 2006). The dimensions of analysis of the responses included in the Dataset are described in the Coding Manual.

The dataset includes the raw data carried out in the work titled "Olive seed protein hydrolysates possess antioxidant and anti-inflammatory activities in mice and human cells". The olive seed protein hydrolysates (OSPHs) demonstrated antioxidant and immunomodulatory effects on RAW 246.7 cells and human peripheral blood mononuclear cells, suggesting their potential for use in developing new functional foods or nutraceuticals. The aim of this work was to deepen the study of multifunctional behavior of OSPHs obtained from the Frantoio cultivar of Olea europaea L., prepared using two food-grade enzymes, namely Alcalase® (AH) and Papain (PH), studying their antioxidant capacity and possible immunomodulatory activity on a murine monocyte/macrophage cell line (RAW 246.7) and on the human peripheric blood mononuclear cells (PBMCs)., The data set titled “OliveSeedProteinHydrolysates_Dataset.xlsx” includes raw data from in vitro experiments.

Existe una amplia variedad de enfoques para medir la calidad metodológica (MQ). Los profesionales, generalmente no expertos en metodología, no tienen criterios claros para evaluar la MQ de los programas de intervención o estudios primarios. Además, las propuestas existentes carecen de evidencia de sus propiedades métricas y no explican los motivos de los indicadores seleccionados. Este estudio propone la Escala de Calidad Metodológica (MQS) como una herramienta simple y útil con fiabilidad, con evidencias de validez y propiedades métricas adecuadas. Se parte de una revisión de la literatura de todos los instrumentos existentes [DataS1] y como resultado final del proyecto se presenta una nueva escala para medir la calidad metodológica [DataS5]. La escala final resultante se aplica sobre un conjunto de estudios primarios sobre ‘organizational training programs’ [DataS3]. Para ello se desarrolló un manual de codificación [DataS2] de tal forma que dos codificadores independientes lo aplicaron para codificar los estudios que estaban en la base de datos de estudios primarios, generando una nueva base de datos [DataS4]. Fechas de generación de las bases de datos: La DataS1 estaba ya disponible como material suplementario en un ‘paper’ publicado en Frontiers: ‘Chacón-Moscoso, S., Sanduvete-Chaves, S., & Sánchez-Martín, M. (2016). The development of a checklist to enhance methodological quality in intervention programs. Frontiers in Psychology, 7: 1811. https://doi.org/10.3389/fpsyg.2016.01811’ - DataS2SupportingInformationCodingManual: 12/4/2020 - DataS3SupportingInformationReference299PrimaryStudies: 22/4/2020 - DataS4SupportingInformationDatabase: 25/5/2021 - DataS5_MQS: 02/3/2022, - DataS1SupportingInformationBibliographyMethodologicalQuality - DataS2SupportingInformationCodingManual - DataS3SupportingInformationReference299PrimaryStudies. - DataS4SupportingInformationDatabase - DataS5_MQS

Datos de caracteres para el análisis morfométrico de especies de interés taxonómico de los géneros Lactuca y Sonchus en la Península Ibérica., 1.Flora_Iberica-Lactuca.perennis.xls; 2.Flora_Iberica-Lactuca.viminea.xls; 3.Flora_iberica-Localidades_Lactuca.viminea.doc; 4.Flora_Iberica-Sonchus.bulbosus.xls

Registro de 24 caracteres (12 cuantitativos y 12 cualitativos), considerados de interés en la literatura taxonómica para la discriminación de Lactuca livida y L. virosa. El estado de los caracteres se registró en 164 pliegos, 1-Lactuca.livida_Morphometrics.xls, 2-Lactuca.livida_variability_florets.xls, 3-Lactuca-livida_mantle_lobation.txt, 4-Lactuca.livida_mantle_florets.txt, 5-Lactuca.livida_OnlineSupplement.word

Dataset obtained for the study published in the paper: “Direct evaporative cooling from wetted surfaces: Challenges for a clean air conditioning solution”. Evaporative cooling has a major role to play in fighting climate change and in achieving a low-carbon economy. Wetted media are commonly called evaporative cooling pads and are widely used in greenhouses, intensive livestock farming, and industrial facilities. These can enhance their application to indoor occupied spaces such as residential or commercial cooling, or in hybrid air conditioning systems. Existing results on these less studied performance issues are reviewed, and we identify the gaps in the literature in addition to highlighting the main challenges encountered, in an effort to guide future researchers in the field and enhance the application of direct evaporative cooling. Existing results on these less studied performance issues are reviewed, and we identify the gaps in the literature, such as power requirements and the coefficient of performance, water consumption, risk of water entrainment, material decay, and air quality, as well as the effect of water temperature and salinity, solar radiation, or wind speed. In addition to highlighting the main challenges encountered, in an effort to guide future researchers in the field and enhance the application of direct evaporative cooling., Dataset whose contents are structured in an Excel table, where each row contains the reference of the paper cited (first author; year) followed by whether it is an experimental work or a simulation/model, type of evaporator panel used, the operating parameters studied as well as the factors analysed that influence these operating parameters and, finally, the most important results found.

Descripción de la nueva especie Sonchus boulosii Chamboul., Mejías & J.F. Léger y de Sonchus L. sectio Pulvinati S.C. Kim, Mejías & Chamboul. Incluye descripción morfológica, análisis filogenético, estudio cromosómico, sistemática, consideraciones evolutivas y biogeográficas, 1.Sonchus_boulosii_MORPHOLOGY.xls, 2.Sonchus_boulosii_CHROMOSOMES.xls, 3.Sonchus_boulosii-Sonchinae_ITS_180509.txt, 4.Sonchus_boulosii-Sonchinae_matk_180509.txt, 5.Sonchus_boulosii_GenBank Accessin, 6. Sonchus_boulosii_Collections