BUSCADOR RECOLECTA

Different cell lines (SAF-1, DLB-1, PLHC-1 and FuB-1) mitro stress (OCR and ECAR) raw data., Different cell lines (SAF-1, DLB-1, PLHC-1 and FuB-1) mitro stress (OCR and ECAR) raw data.

Ultra Performance Liquid Chromatography coupled with a high-resolution quadrupole-orbitrap mass spectrometry DATA

Silk fibroin microparticles (SF) were administered in the diet of gilthead seabream with or without experimental skin wounds to study the effects on cellular immunity and liver. A commercial diet was enriched with different SF contents: 0, 50 and 100 mg kg-1 (control, SF50 and SF100 diet, respectively). The animals were fed for 30 days and half of them were sampled. Similar experimental wounds were then performed to the rest of fish and they continued to be fed the same diet. At 7 days post-wounding, samples were taken from the wounded fish. Cellular immunity was studied on head kidney leucocytes (phagocytosis, respiratory and peroxidase content) and liver status (histological study and gene expression) were studied. Our results showed that experimental wounds affect both cellular immunity (by decreasing leucocyte respiratory burst and peroxidase activity) and altered liver histology (by inducing vascularization and congestion of blood vessels). In addition, it affects the expression of genes indicative of oxidative stress, endoplasmic reticulum stress and apoptosis. More specifically, it was the highest dose of silk fibroin (SF100) that significantly increased the phagocytic capacity of leucocytes as well as vascularization in the liver. In addition, increased expression of antioxidant genes (cat and gsr) and decreased expression of genes related to reticulum endoplasmic stress (grp94 and grp170) and apoptosis (nos and jnk) were detected in these fish fed with SF100 and wounded. In conclusion, fed fish with SF100 had many beneficial effects as cellular immunostimulant and hepatoprotection in wounded fish. Its use could be of great interest for stress management in farmed fish conditions.

The specific fatty acid profile of fish can vary depending on species, age, diet and other factors. However, in general, fish are generally rich in long-chain polyunsaturated fatty acids (LC-PUFA), such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Liver, skin, meat and fish mucus can contain different levels and types of fatty acids. In fact, each of these tissues can provide valuable information about the fatty acid profile of the whole fish. In order to establish a non-invasive method to assess the lipid profile of fish tissues, twenty-four European sea bass (Dicentrarchus labrax) were fed two different diets [containing different PUFAs profiles] for 30 days. Samples of cutaneous mucus, skin, flesh and liver were then obtained and their fatty acid profile was analyzed by gas chromatography. Multidimensional scaling (MDS), SIMPER procedure (similarity percentages) and permutation test (PERMANOVA) were performed. The results indicate that there are similarities in the fatty lipid profile of skin and liver, as well as between skin and liver mucus. Relationships have been observed between the fatty acid compositions of the different fish tissues, in particular between liver and skin, where linoleic acid (18:2n-6) is the most significant, which would allow the skin to be used as a proxy to infer the hepatic lipid profile, without the need to sacrifice the animals