Resultados de investigación

The dataset contains the results of the trials of nest detectability using 25 volunteers as "experimental conspecifics” to estimate the detectability of black kite nests to trespassers. The dataset include the ID of Volunteers; Id of the images; ID of the nest; Distance to the nest (measured in AGL and meters); Position of the UAV when taking the image (zenithal, lateral or approaching snapshots); Decoration of the nests (Decorated (1) vs. non-decorated (0)); Detection of the nest by the volunteer (Detected (1) vs. non-detected (0)); Time to detection (seconds); Detection_pair (coded as 1, if the images from the same position and both treatments were detected in a nest, or 0, in the opposite case) and nest dimensions: lenght, width and opening angles, defined as the three angles of unobstructed view of the sky (see main text for further details), In this study, we used UAS (Unmanned Aircraft Systems) technology to simulate the aerial perspective of trespassing, flying black kites, and assess whether decorated nests were more conspicuous than undecorated ones to a human observer. To this end, we flew at pre-determined distances from actual nests built by black kites a UAS, equipped with a digital high-resolution camera, and gathered images of the nests with and without an experimentally placed decoration. The images were later standardized using ad hoc prepared software and shown to volunteers through a standardized routine to determine whether detection rate varied according to nest decoration status and distance, Peer reviewed

The data are organized into two Excel files: 1. dead vs alive GPS-tags.xls. This file includes data on radio-tagged individuals that were classified as surely dead or surely alive, together with the GPS-satellite tags-parameters used to predict their dead or alive status. 2. dead vs alive Doppler-tags.xls. This file includes data on radio-tagged individuals that were classified as surely dead or surely alive, together with the Doppler tags-parameters used to predict their dead or alive status., Peer reviewed

The data are organized into an .inp file, which includes data on the fates of Red kites (Milvus milvus) ringed as chicks in the nest in Doñana National and Natural Park. Each data column represents the capture history of each individual. Values of 1 indicate individuals observed to be alive. Values of zero indicate individuals that were either dead or not observed in a given year, This dataset incorporates data on the survival of Red kites (Milvus milvus) marked with PVC rings as chicks in the nest., Peer reviewed

The data are organized into 10 spreadsheets within a single Excel file on the impact of drought on: prey availability, nestlings' provisioning rates, probability of breeding, clutch size, hatching success, brood reduction, predation rate, number of young fledged per breeding pair, number of young fledged per territorial pair, body condition, body size., Part of the study was funded by Foundation Jaime González-Gordon and by the research projects 1602/2015 of the Spanish Ministry of Agriculture, Food and the Environment (Autonomous Organism of National Parks), PGC2018-095860-B-I00 of the Ministry of Science, Innovation and Universities with Feder Funds, and P18-FR-4239 of the Andalucía Autonomous Region (Consejería de Economía, Conocimiento, Empresas y Universidad)., Peer reviewed

The data are organized into seven Excel files on how black kites negotiate wind conditions when they: depart for migration (depart for migration.xlsx), travel and compensate for lateral drift (drift.xlsx), travel and negotiate axial winds (forward.xlsx), stop for a staging stop over (stop for stopover.xlsx), retake their migratory journey after a stopover (start from stopover). Two further Excel files examine how wind negotiation improves within the individual (improvements.xlsx) and is subject to mortality selection (survival & slope.xlsx)., This dataset incorporates data on wind negotiation and response to drift by migratory black kites., Part of the study was funded by Natural Research Ltd and research projects CGL2008-01781 (F.S.), CGL2011-28103 (F.S.), CGL2012-32544 (J.B.) and PGC2018-095860-B-I00 (F.S.) of the Spanish Ministry of Science and Innovation/Economy and Competitiveness and FEDER funds, 511/2012 (J.B.) of the Spanish Ministry of Agriculture, Food and the Environment (Autonomous Organism of National Parks), JA-58 (F.S.) of the Consejería de Medio Ambiente de la Junta de Andalucía and by the Excellence Projects RNM 1790 (F.S.), RNM 3822 (F.S.), RNM 7307 (F.S.) and P18-FR-4239 (F.S.) of the Junta de Andalucía. J.M.B was supported by Generalitat Valenciana (CIDEGENT/2020/030)., File List: Depart for migration.xls, Depart for stopover.xls, Drift.xls Forward.xls, Improvements.xls, Stop at stopover.xls, Survival & slope.xls., Peer reviewed

Eukaryotic chromosome segregation relies on the assembly of a bipolar machinery based on microtubules (MTs), named the mitotic spindle. Formation of the mitotic spindle follows a force balance mechanism that ensures the proper capture and separation of sister chromatids. Many proteins have been involved in the establishment of this force balance, although kinesin 5 is well recognized as the major outward pushing force generator, since its inactivation results in monopolar, non-functional spindles. In order to find additional players in the force balance mechanism, we have performed a suppressor screen using a conditional allele of the fission yeast kinesin 5 ortholog Cut7. This screen identified that the lack of the PP6 phosphatase partially suppresses cut7 phenotypes, at least by defective translation of MT regulators, such as the minus end-directed kinesin Klp2, the MT stabilizer Alp7 and the MT bundler Ase1, impacting on the force balance mechanism. Additionally, our data show that the Elongator complex, a target activated by PP6 for efficient tRNA modification, also contributes to the force balance, albeit to a lesser extent. Importantly, this complex has recently been implicated in direct MT polymerization in metazoans, a role not shared by its fission yeast counterpart., Peer reviewed

Eukaryotic chromosome segregation relies on the assembly of a bipolar machinery based on microtubules (MTs), named the mitotic spindle. Formation of the mitotic spindle follows a force balance mechanism that ensures the proper capture and separation of sister chromatids. Many proteins have been involved in the establishment of this force balance, although kinesin 5 is well recognized as the major outward pushing force generator, since its inactivation results in monopolar, non-functional spindles. In order to find additional players in the force balance mechanism, we have performed a suppressor screen using a conditional allele of the fission yeast kinesin 5 ortholog Cut7. This screen identified that the lack of the PP6 phosphatase partially suppresses cut7 phenotypes, at least by defective translation of MT regulators, such as the minus end-directed kinesin Klp2, the MT stabilizer Alp7 and the MT bundler Ase1, impacting on the force balance mechanism. Additionally, our data show that the Elongator complex, a target activated by PP6 for efficient tRNA modification, also contributes to the force balance, albeit to a lesser extent. Importantly, this complex has recently been implicated in direct MT polymerization in metazoans, a role not shared by its fission yeast counterpart., Peer reviewed

A: Serial dilution assay of the wild type, ekc1∆, cut7–24 and cut7–24 ekc1∆ strains incubated for 3 days at the indicated temperatures. B: SDS PAGE analysis of Cut7-3xHA levels on wild type or ppe1∆ cells. The upper panel corresponds to the blot incubated with an anti-HA antibody, while the bottom panel corresponds to the blot incubated with an anti-tubulin antibody. C: Plot showing the total Cut7-3xHA levels relative to the corresponding tubulin levels in wild type and ppe1∆ cells. n for wild type: 3; n for ppe1∆: 3. a.u.: arbitrary units. Statistical significance was determined using a T-test (p = 0.086). D: Upper panel, PhosTag SDS PAGE analysis of Cut7-3xHA in asynchronous and mitotically blocked nda3-KM311 cells in the presence or absence of Ppe1. Bottom panel, the same samples submitted to SDS PAGE analysis using an anti-tubulin antibody. E: SDS PAGE analysis of Pkl1-13xMyc levels on wild type or ppe1∆ cells. The upper panel corresponds to the blot incubated with an anti-Myc antibody, while the bottom panel corresponds to the blot incubated with an anti-tubulin antibody. F: Plot showing the total Pkl1-13xMyc levels relative to the corresponding tubulin levels in wild type and ppe1∆ cells. n for wild type: 3; n for ppe1∆: 3. a.u.: arbitrary units. Statistical significance was determined using a T-test (p = 0.121). G: Upper panel, PhosTag SDS PAGE analysis of Pkl1-13xMyc in asynchronous and mitotically blocked nda3-KM311 cells in the presence or absence of Ppe1. Bottom panel, the same samples submitted to SDS PAGE analysis using an anti-tubulin antibody., Peer reviewed

A: Serial dilution assay of the wild type, elp3∆, cut7–24, cut7–24 elp3∆, cut7–22 and cut7–22 elp3∆ strains incubated for 3 days at the indicated temperatures. B: Selected kymographs showing interphasic MT dynamics in wild type, ppe1∆, elp3∆ and elp3-YY527AA cells expressing GFP-Atb2, showing the inverted green channel in a grey scale. Scale bar: 5 μm. C: Plot showing MT growth rate of the indicated strains expressing GFP-Atb2 (n for wild type: 74; n for ppe1∆: 87; n for elp3∆: 79; n for elp3-YY527AA: 79). Statistical differences were determined using a T-test (wt vs. ppe1∆, p = 0.409; wt vs. elp3∆, p = 0.374; wt vs. elp3-YY527AA, p = 0.001). D: Plot showing MT catastrophe rate of the indicated strains expressing GFP-Atb2 (n for wild type: 95; n for ppe1∆: 73; n for elp3∆: 82; n for elp3-YY527AA: 88). Statistical differences were determined using a T-test (wt vs. ppe1∆, p = 0.054; wt vs. elp3∆, p = 0.003; wt vs. elp3-YY527AA, p = 0.294). E: Plot showing MT dwelling time at the cell end of the indicated strains expressing GFP-Atb2 (n for wild type: 63; n for ppe1∆: 67; n for elp3∆: 67; n for elp3-YY527AA: 65). Statistical differences were determined using a T-test (wt vs. ppe1∆, p = 0.931; wt vs. elp3∆, p = 0.500; wt vs. elp3-YY527AA, p = 0.667). F: Maximum intensity projections from confocal time lapse images of wild type cells expressing mCherry-Atb2 and Elp3 or Elp4 fused to GFP. The upper series shows the magenta (mCherry-Atb2) and green (Elp3-GFP or Elp4-GFP) merged channels. The bottom series shows the inverted green channel in a grey scale. Time 0 corresponds to mitotic entry. The dashed line represents the cell contour. Scale bar: 2 μm. G: Western-blot analysis of SDS PAGE of Elp3-GFP and Elp4-GFP from the strains shown in C., Peer reviewed

A: Serial dilution assay of the wild type, ppe1∆, klp2∆, ppe1∆ klp2∆, cut7–22, cut7–22 ppe1∆, cut7–22 klp2∆ and cut7–22 ppe1∆ klp2∆ strains incubated for 3 days at the indicated temperatures. B: Serial dilution assay of the wild type, ppe1∆, alp7∆, ppe1∆ alp7∆, cut7–22, cut7–22 ppe1∆, cut7–22 alp7∆ and cut7–22 ppe1∆ alp7∆ strains incubated for 3 days at the indicated temperatures. C: Serial dilution assay of the wild type, ppe1∆, ase1∆, ppe1∆ ase1∆, cut7–22, cut7–22 ppe1∆, cut7–22 ase1∆ and cut7–22 ppe1∆ ase1∆ strains incubated for 3 days at the indicated temperatures., Peer reviewed