Resultados de investigación

This Electronic Supporting Material contains details about the Experimental section as well as some additional comments about Results and Discussion section. The Experimental section describes the reagents employed for the synthesis and characterization of the IrNCs and IrNCs-immunoprobes, the culture and incubation of ARPE-19 cells, the immunocytochemistry (ICC) assays for the detection of target proteins by MS and fluorescence, the cellular preparation for sc-ICP-MS and LA-ICP-MS, as well as the ELISA analyses. Furthermore, protocols for the synthesis and characterization of IrNCs and IrNCs-immunoprobes are also included. In the Results and Discussion section, results regarding the optimization of the glucose-induced oxidative stress treatment, the determination of proteins in individual ARPE-19 cells by sc-ICP-MS, as well as the bioimaging of APOE and claudin-1 in individual ARPE-19 cells by LA-ICP-MS are also collected., Peer reviewed