Resultados de investigación

Supplementary Fig. 1. Schematic representation of the peptide array. Supplementary Fig. 2. Screening of the antibodies present in the pre-immune bleed and in four post-immunization bleeds collected from a sheep immunized with HNE-modified HSA. Supplementary Fig. 3. SA369 anti-HSA-HNE pAb enriched pAb purification chromatograms from affinity purification using an HSA-HNE-coupled resin to recover the mixture of anti-HA and anti-HSA-HNE IgGs (A) and an HSA-coupled resin for depletion of anti-HSA antibodies (B). Supplementary Fig. 4. Chromatogram obtained from the purification of the IgGs from the crude sheep sera (SA369) with a protein G resin (A) and from the enrichment of anti-HNE antibodies using a KLH-HNE conjugated resin (B). Supplementary Figure 5. Purification of SA369 pAbs from the sheep bleed SA369 using a KLH-HNE covalently bound to a sepharose resin. Supplementary Figure 6. ELISA assay comparing the binding of the anti-HNE Abcam and the Anti-HSA-HNE SA369 antibodies to recombinant HSA expressed in Saccharomyces cerevisiae and standard HSA (the same HSA molecule used for immunization). Supplementary Table 1. List of the human serum albumin (UniProt ID: P02768) peptides synthesized on the arrays, Supplementary data for the article https://doi.org/10.1016/j.freeradbiomed.2019.05.008, Peer reviewed