BUSCADOR RECOLECTA

1 table., The table includes for each miRNA the arguments for its selection for further validation, the forward and reverse sequence, the miRBase sequence used as template for primer design, if successful miRNA amplification was obtained with qPCR and qPCR amplification efficiency., Peer reviewed

1 table, Total RNA was isolated from whole urine of 38 cats, and 6 μl of each RNA sample was applied in small RNAseq. Concentration and ratios were obtained using Nanodrop measurement., Peer reviewed

1 table., Reads mapped to the different gene biotypes in the genome are included. SD: standard deviation; rRNA: ribosomal RNA; tRNA: transfer RNA; snoRNA: small nucleolar RNA; snRNA: small nuclear RNA; RE: repeat elements; miRNA: microRNA., Peer reviewed

1 table., mirDeep2 output of the 996 miRNAs identified with information from their sequences, scores and probabilities of being a true positive miRNA, among others., Peer reviewed

1 table., Including the miRNA ID name based on sequence homology with other species (blastn), miRNA coordinates, strand, closest homology miRNA with their percentage of identity and probability (E-value) and consensus mature, star and precursor sequences., Peer reviewed

1 table., This includes information from their source (database/study/de novo) and their genomic coordinates. CPM: Counts Per Million., Peer reviewed

A. List of differentially abundant (DA) miRNAs between the Control group (healthy) and the different uropathological conditions and between PN and the other uropathological conditions using RNAseq data. B. List of all miRNAs comparisons (without q-value and log2FC filters). The values of log2FC are calculated using the Controls as baseline and therefore a positive log2FC implies an upregulation of the specific miRNA in the pathological state and vice versa. Accordingly, in the comparison PN vs Other pathologies, the PN group was set as baseline, and a negative log2FC thus indicates up-regulation in PN compared to other pathologies. log2FC: log2 of the fold-change in expression. CKD: Chronic Kidney Disease; SB/C: Subclinical Bacteriuria/Cystitis; PN: Pylonephritis; UO: Ureteral Obstruction., Peer reviewed

1 table., The values of log2FC are calculated using the Control cats as baseline, and, therefore, a positive log2FC implies an up-regulation of the specific miRNA in the pathological state and vice versa. Accordingly, in the comparison PN vs. other pathologies, the PN group was set as baseline, and a negative log2FC thus indicates up-regulation in PN compared to other pathologies. In dark grey DA miRNA surviving the criteria |log2FC| ≥ 1.5 and q-value < 0.05; in light grey DA miRNAs surviving the criteria |log2FC| ≥ 1.5 and p-value < 0.05; CKD: Chronic kidney disease; SB/C: Subclinical bacteriuria/Cystitis; PN: Pyelonephritis; UO: Ureteral obstruction, FDR: False discovery rate., Peer reviewed

1 table., Pathway enrichment of the KEGG and Reactome terms are shown if they gathered significant false discovery rate (FDR) and corrected p-values (q-value < 0.05); their associated mRNA genes found are also shown., Peer reviewed

1 table., Phenotypic values of n-6/n-3 PUFA ratio, sex classification, and batch grouping recorded in 20 Iberian x Duroc pigs, Peer reviewed