BUSCADOR RECOLECTA

S1. Synthesis of cycloalkyne ligand S2. Synthesis of RGD azide S3. Reaction yields and spectroscopic characterization of the synthesized compounds Tables and Figures, Peer reviewed

Synthesis, functionalization, and characterization of the NPs, stability of the NPs in diverse media, further details of the PC study and in vitro degradation study, fluorescent examination of tissue sections, in vivo toxicity study, and EDC of the NPs in the liver and spleen., Peer reviewed

Table S1. Hydrodynamic size and ζ-potential of magnetic nanoparticles. Table S2. Iron concentration located inside the cells. Figure S1. Sterility assay. Figure S2. MNP toxicity assay in 2D cell cultures. Figure S3. MNP toxicity evaluated in 3D models. Figure S4. Magnetic characterization of the 3D models., Peer reviewed

The dataset consists of the prediction of transcription factor binding sites (ChIP-seq data) and transcription factor binding motifs (Position weight matrices and Transcription factor flexible models) in Drosophila melanogaster and humans., Although transposable elements (TEs) are an important source of regulatory variation, their genome-wide contribution to the transcriptional regulation of stress response genes has not been studied yet. Stress is a major aspect of natural selection in the wild, leading to changes in the transcriptional regulation of a variety of genes that are often triggered by one or a few transcription factors. In this work, we take advantage of the wealth of information available for Drosophila melanogaster and humans to analyze the role of TEs in stress regulatory networks. We used in silico predictions and chromatin immunoprecipitation sequencing (ChIP-seq) data to localize transcription factor binding sites (TFBS) for several stress-response transcription factors involved in six stress regulatory networks: immune, hypoxia, oxidative, xenobiotic, heat shock, and heavymetal stress responses. The predictions at the DNA level were complemented with the analysis of biochemical and genomic features, such as chromatin characteristics and location regarding nearby genes. In addition, we also used population level information to identify a subset of TEs that were more likely to contain functional TFBS. For a representative subset of the most likely candidate TEs, we performed in vivo transgenic reporter assays in different stress conditions. Overall, our results showed that TEs are relevant contributors to the transcriptional regulation of stress response genes., This work was funded by the Ministerio de Economia y Competitividad BFU2014-57779-P, Ministerio de Ciencia, Innovación y Universidades/AEI (BFU2017-82937-P) and the European Commission (H2020-ERC-2014-CoG-647900)., Peer reviewed

WC4PopStr_26112018.vcf.gz: Contains SNP calligs for the 83 genomes (26 Swedish, 16 Italian, 24 North American, and 17 African) after filtering SNPs at regions of known cosmopolitan admixture in the African strains (3749072 SNPs).-- EUPopStr_26112018.vcf.gz: Contains SNP callings for the new 42 genomes (26 Swedish, 16 Italian) sequenced in this work. (extracted from WC4PopStr_26112018.vcf.gz)., Peer reviewed

VCF file containing allele variant information of the 48 Drosophila melanogaster samples collected in 32 different locations and 13 countries in Europe over 2014 by the DrosEU consortium members., Peer reviewed