BUSCADOR RECOLECTA

Pru p 3-specific IgE values obtained by ImmunoCAP in LTP-allergic patients and tolerant controls., Peer reviewed

The difference in the localization / internalization of the toll-like receptors was determined by confocal microscopy and flow cytometre. These differences between both was due to the differential location of them, since the expression of TLR4 is at the cell surface, while TLR7 is at intracellular compartments, Peer reviewed

Peer reviewed

Data showed are changes in the maturation index of CD83, CD86 and PD-L1 in allergic patient and tolerant control., Peer reviewed

Production of cytokine into the culture supernatant. In the case of TLR4, only significant increases of IL-5 were observed in allergic patients compared to tolerant controls when stimulating with TLR4lig-Pp3 and TLR4lig plus Pp3 peptide in separately way. No differences in the induction of IL-10 production were observed in allergic patients compared to tolerant controls, Peer reviewed

Production of cytokine into the culture supernatant from DCs. Our analysis in allergic patients compared to tolerant controls detected significant increases of IL-5 and IFN with all the conditions containing TLR7lig (TLR7lig, TLR7lig-Pp3 and TLR7lig plus Pp3 in separately way). we observed that TLR7lig-Pp3 induced significant lower IL-4 and IL-5 levels whilst higher IFNγ levels compared to the Pp3 only in allergic patients, indicating the specific immunomodulatory capacity of this compound., Peer reviewed

Data indicated that only TLR7lig-Pp3 led to significantly increased in the proliferation index of CD3+T-cells in allergic patients compared to tolerant controls, while TLR4lig-Pp3 seemed stimulate the T-cells proliferation without inducing a difference between groups. Moreover, in allergic patients there was a significant increase in CD3+ and CD4+ T-cells proliferation after stimulation with TLR4lig-Pp3 vs TLRlig and vs TLR4lig plus Pp3 peptide; additionally, TLR7lig-Pp3 induced a specific proliferative response of CD3+T-cells compared to TLR7lig and TLR7lig plus Pp3 peptide., Peer reviewed

Regarding the cytokine profile produced during the proliferative response, the most interesting results indicated that both TLRnlig-Pp3 induced a decrease of IL-4 and IL-5 levels compared to Pp3 peptide (alone), being significant for IL-5 with TLR4lig-Pp3, and an increase of IFN being significant only for TLR7lig-Pp3 in allergic patients., Peer reviewed

Data indicated an opposite result, since the combination of both TLRnlig-Pp3 (TLR7lig-Pp3 plus TLR4lig-Pp3) together decreased the maturation already produced by each compound alone, being significant for CD80, Peer reviewed

Regarding the effect of these interactions with DC on the proliferative response of the T-, B- and NK-cells, there was a decrease in comparison with the proliferation induced by the ligands incubated separately, Peer reviewed