BUSCADOR RECOLECTA

Supplementary_File_1_HP1_peaks Supplementary_File_2_HP1_gene_tissues Supplementary_File_3_Overlapping between HP1 bound genes with tissue-specific genes Supplementary_File_4_Overlapping between tissue-specific genes bound by HP1 proteins Supplemental Figures and Tables, Peer reviewed

pbio.3002315.t001 - Lineage tracing identifies heterogeneous hepatoblast contribution to cell lineages and postembryonic organ growth dynamics, Peer reviewed

Schematics showing the current working models: (A) uni- and bipotent hepatoblast contributions to hepatocytes and BECs following heterogeneous lineage decisions. (B) Hepatoblasts contribute with heterogeneous proliferation behaviours to postembryonic liver growth. Cells from the embryonic left lobe contribute to the ventral lobe, including the formation of giant clusters (magenta). (C) The liver morphology changes dramatically simultaneous to the intestinal bending occurring during postembryonic growth (green). BEC, biliary epithelial cell; dpf, day postfertilization; SL, standard length., Peer reviewed

(A) The 6 steps of ventral liver lobe formation correlate with fish standard length (SL). The numerical values that were used to generate the graph can be found in S1 Data. (B) Stage I: A small tissue extension at the tip of the left lobe is visible (n = 12 livers). (C) Stage II: a thin ventral lobe originates in the lower half of the left lobe (n = 6 livers). (D) Stage III: the thin ventral lobe shifts position towards the more anterior part of the left lobe (n = 4 livers). (E) Stage IV: the tip of the ventral lobe starts to expand (n = 7 livers). (F) Stage V: lateral-oriented expansion of the ventral lobe (n = 28 livers). (G) Stage VI: enlargement of all lobes in width (n = 6 livers). The blue areas in the schematics mark the region characteristic for the respective stage. (H) Schematic depicting the morphology of the liver in relation to the folding of the intestine in stages I-VI. A, anterior; P, posterior; R, right; L, left; RL, right lobe; LL, left lobe; VL, ventral lobe., Peer reviewed

(A) Whole-mount of a 14-dpf zebrafish liver, displaying sparse multinucleated hepatocytes (N = 1, n = 2 livers; yellow arrowheads indicate binucleated cells). (B) Approximately 5 μm projection of a region of a juvenile liver. Fish SL = 11.16 mm (N = 3, n = 6 livers). (C) Segmentation shows variable nuclear volumes, which correlate with the sum intensity of DAPI, indicating that bigger nuclei have a higher amount of DNA (D). (E) Approximately 5 μm projection of an adult liver region (N = 3, n = 3 livers). (F) Segmented nuclei show only sparse variability in volume, with few bigger nuclei. Nuclear volume correlates with sum intensity of DAPI (G). (H) Schematics representing the transient appearance of polyploid cells over time; blue trajectory is manually approximated based on qualitative analysis. The numerical values that were used to generate the graphs in (D, G, H) can be found in S1 Data. DAPI, 4′,6-diamidino-2-phenylindole; dpf, day postfertilization; SL, standard length., Peer reviewed

Uncropped gel images of S2E and Uncropped gel images of S2F Fig., Peer reviewed