BUSCADOR RECOLECTA

Predicted genes corresponding to the four most common enzymes present in photosynthetic organisms: NADH:ubiquinone reductase (H+-translocating), N-acetyl-gamma-glutamyl-phosphate reductase, DNA-directed RNA polymerase and non-specific serine/threonine protein kinase of 174 metagenomes sequenced during the Malaspina 2010 global expedition., Peer reviewed

This dataset is composed of 4 files. The main ones are the dataset itself (‘BBMO_database_2009-2022_hy1.csv’ and ‘EOS_database_2010-2019_hy1.csv’) in WHP-Exchange bottle format, with temperature, salinity, dissolved inorganic nutrient concentrations, pH, and total alkalinity data. Information about the time-series name, date, and location (latitude and longitude) is also provided. Information about the equipment used for seawater carbonate system data is also given. The other two files (‘README_BBMO_database_2009-2022.txt’ and ‘README_EOS_database_2010-2019.txt’) include a short description of the database variables, units and more details on the time-series data. The data are provided under an Attribution-ShareAlike 4.0 International license. However, if you use the data, so as to support the authors, please consider citing the above-mentioned article where data collection and analytical techniques are given in detail. Here we only provide an overall description of the content of the data file., [EN] The database provides discrete measurements of seawater carbonate system variables (pH and total alkalinity) and ancillary variables (dissolved inorganic nutrients, temperature, salinity, and pressure) in surface seawater collected at two time-series stations, EOS and BBMO, conducted over 2009–2022. The database compiles all the information, measurements, and analysis performed (including date, location, and equipment used). All chemical analyses were assigned a quality indicator. At the two time-series stations, monthly sampling of surface seawater was performed. BBMO temperature and salinity were measured with a SAIV-A/S-SD204 CTD, while at EOS temperature was measured with reversible thermometers and salinity was measured with two CTDs, a SAIV-A/S-SD204 CTD and a CTD75M. For consistency between BBMO and EOS datasets, the salinity record obtained with the SAIV-A/S-SD204 CTD is considered in this study. When salinity values from the SAIV-A/S-SD204 CTD were missing, salinity values from CTD75M were used. The salinity records obtained with the two CTDs were quality controlled and calibrated to obtain a consistent dataset. Discrete seawater samples for pH, TA, and dissolved inorganic nutrients were taken monthly from a depth of 0.5 m both stations (with a Niskin bottle at EOS and in 10 L polyethylene carboys at BBMO). The biogeochemical variables shown in the database are pH reported on the total hydrogen ion scale at 25 °C (PH_TOT), total alkalinity (ALKALI), and dissolved inorganic nutrients (phosphate, PHSPHT, Silicate, SILCAT, and Nitrate, NITRAT). PH_TOT data were obtained by the spectrophotometric method with m-cresol purple using a Cary 100 UV-vis spectrophotometer containing a 25 °C-thermostated cells holder following Clayton and Byrne (1993). Triplicate samples were taken. At EOS, three cylindrical optical glass cells with a 10 cm path-length were filled directly from the Niskin bottle right after reaching land, ready to be analyzed in the next 3–4 hrs. At BBMO, a 150 mL glass bottle was filled leaving no headspace, and three 10 cm path-length cylindrical optical glass cells were filled from it once in the ICM laboratory (< 2h after sampling). For ALKALI measurements, two samples per site were taken in 500 mL borosilicate glass bottles were filled, rinsed three times, and carefully filled from the bottom with a tube. Samples for ALKALI were poisoned with 300 µL mercuric chloride (HgCl2) saturated solution to halt biological activity (Dickson et al., 2007), right after reaching land for EOS samples and at the ICM laboratory for BBMO samples. Bottles were then stored in the dark at room temperature until they were measured in the laboratory, within days up to one month. Each ALKALI sample was measured by duplicate. ALKALI was analyzed by potentiometric titration, determined by double endpoint titration (Mintrop et al., 2000; Pérez and Fraga, 1987), and calibrated with reference materials (Prof. A. Dickson, Scripps Institution of Oceanography (USA); Batches #93, #104, #136, and #179). Samples for dissolved inorganic nutrients were stored frozen at -20 °C in sterile falcon tubes until analysis. These tubes were filled directly from the Niskin bottle upon reaching land in EOS and from a bottle at the ICM laboratory in the case of BBMO samples. Dissolved inorganic nutrients were determined by standard continuous flow analysis with colorimetric detection (Hansen and Grassof, 1983) using a Bran + Luebbe autoanalyser., This research was funded by the European Union – NextGeneration EU – as part of the MITECO program for the Spanish Recovery, Transformation and Resilience Plan (Recovery and Resilience Facility of the European Union established by the Regulation (EU) 2020/2094), entrusted to CSIC, AZTI, SOCIB, and the universities of Vigo and Cadiz. Sampling has been funded by multiple projects of the Spanish Ministry of Science. Financial and institutional support was also received from the Catalan Government (Research Group on Marine Biogeochemistry and Global Change, 2021SGR00430) and from the ‘Severo Ochoa Centre of Excellence’ (CEX2019-000928-S) funded by AEI 10.13039/501100011033, which included a postdoctoral contract to M.I.G.-I., Peer reviewed

Pru p 3-specific IgE values obtained by ImmunoCAP in LTP-allergic patients and tolerant controls., Peer reviewed

The difference in the localization / internalization of the toll-like receptors was determined by confocal microscopy and flow cytometre. These differences between both was due to the differential location of them, since the expression of TLR4 is at the cell surface, while TLR7 is at intracellular compartments, Peer reviewed

Peer reviewed

Data showed are changes in the maturation index of CD83, CD86 and PD-L1 in allergic patient and tolerant control., Peer reviewed

Production of cytokine into the culture supernatant. In the case of TLR4, only significant increases of IL-5 were observed in allergic patients compared to tolerant controls when stimulating with TLR4lig-Pp3 and TLR4lig plus Pp3 peptide in separately way. No differences in the induction of IL-10 production were observed in allergic patients compared to tolerant controls, Peer reviewed

Production of cytokine into the culture supernatant from DCs. Our analysis in allergic patients compared to tolerant controls detected significant increases of IL-5 and IFN with all the conditions containing TLR7lig (TLR7lig, TLR7lig-Pp3 and TLR7lig plus Pp3 in separately way). we observed that TLR7lig-Pp3 induced significant lower IL-4 and IL-5 levels whilst higher IFNγ levels compared to the Pp3 only in allergic patients, indicating the specific immunomodulatory capacity of this compound., Peer reviewed

Data indicated that only TLR7lig-Pp3 led to significantly increased in the proliferation index of CD3+T-cells in allergic patients compared to tolerant controls, while TLR4lig-Pp3 seemed stimulate the T-cells proliferation without inducing a difference between groups. Moreover, in allergic patients there was a significant increase in CD3+ and CD4+ T-cells proliferation after stimulation with TLR4lig-Pp3 vs TLRlig and vs TLR4lig plus Pp3 peptide; additionally, TLR7lig-Pp3 induced a specific proliferative response of CD3+T-cells compared to TLR7lig and TLR7lig plus Pp3 peptide., Peer reviewed

Regarding the cytokine profile produced during the proliferative response, the most interesting results indicated that both TLRnlig-Pp3 induced a decrease of IL-4 and IL-5 levels compared to Pp3 peptide (alone), being significant for IL-5 with TLR4lig-Pp3, and an increase of IFN being significant only for TLR7lig-Pp3 in allergic patients., Peer reviewed